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Development, maturation and germination of horse chestnut somatic embryos

(2009) Acta Horticulturae. 812. p.301-304
Author
Organization
Abstract
Somatic embryogenesis could represent a way to clonally propagate mature elite trees of horse chestnut. However, while a number of protocols describe successfully the induction and proliferation of somatic embryos a low frequency of embryos converted successfully into plants. The objective of this research was to identify a maturation medium that could contribute to the outcome of more efficient germination results. Therefore, in a first approach the effects of sucrose 3 and 6%, and maltose 3%, were evaluated on somatic embryo maturation, in terms of subsequent somatic embryo germination. Carbon source and concentration had a marked influence on maturation, and subsequent germination of horse chestnut somatic embryos, with sucrose 6% promoting the highest frequency of germinated embryos larger than 1 cm.
Keywords
Aesculus hippocastanum, EXPLANTS, somatic embryogenesis, EMBRYOGENESIS, AESCULUS-HIPPOCASTANUM L, osmoticum, embryo maturation

Citation

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Chicago
Troch, Veronique, Nele Vermeir, Stefaan Werbrouck, and Marie-Christine Van Labeke. 2009. “Development, Maturation and Germination of Horse Chestnut Somatic Embryos.” In Acta Horticulturae, ed. A Romano, 812:301–304. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Troch, V., Vermeir, N., Werbrouck, S., & Van Labeke, M.-C. (2009). Development, maturation and germination of horse chestnut somatic embryos. In A. Romano (Ed.), Acta Horticulturae (Vol. 812, pp. 301–304). Presented at the 3rd International Symposium on Acclimatization and Establishment of Micropropagated Plants, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Troch V, Vermeir N, Werbrouck S, Van Labeke M-C. Development, maturation and germination of horse chestnut somatic embryos. In: Romano A, editor. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2009. p. 301–4.
MLA
Troch, Veronique, Nele Vermeir, Stefaan Werbrouck, et al. “Development, Maturation and Germination of Horse Chestnut Somatic Embryos.” Acta Horticulturae. Ed. A Romano. Vol. 812. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2009. 301–304. Print.
@inproceedings{4280625,
  abstract     = {Somatic embryogenesis could represent a way to clonally propagate mature elite trees of horse chestnut. However, while a number of protocols describe successfully the induction and proliferation of somatic embryos a low frequency of embryos converted successfully into plants. The objective of this research was to identify a maturation medium that could contribute to the outcome of more efficient germination results. Therefore, in a first approach the effects of sucrose 3 and 6\%, and maltose 3\%, were evaluated on somatic embryo maturation, in terms of subsequent somatic embryo germination. Carbon source and concentration had a marked influence on maturation, and subsequent germination of horse chestnut somatic embryos, with sucrose 6\% promoting the highest frequency of germinated embryos larger than 1 cm.},
  author       = {Troch, Veronique and Vermeir, Nele and Werbrouck, Stefaan and Van Labeke, Marie-Christine},
  booktitle    = {Acta Horticulturae},
  editor       = {Romano, A},
  isbn         = {9789066050877},
  issn         = {0567-7572},
  keyword      = {Aesculus hippocastanum,EXPLANTS,somatic embryogenesis,EMBRYOGENESIS,AESCULUS-HIPPOCASTANUM L,osmoticum,embryo maturation},
  language     = {eng},
  location     = {Faro, Portugal},
  pages        = {301--304},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Development, maturation and germination of horse chestnut somatic embryos},
  url          = {http://www.actahort.org/books/812/812\_41.htm},
  volume       = {812},
  year         = {2009},
}

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