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In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

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Abstract
A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l(-1) in combination with 2 mg l(-1) alpha-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l(-1) 6-tau-tau-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia.
Keywords
somatic embryo induction, tubers, callus formation, in vitro culture, Araceae

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Chicago
Duquenne, Barbara, Tom Eeckhaut, Stefaan Werbrouck, and Johan Van Huylenbroeck. 2006. “In Vitro Somatic Embryogenesis and Plant Regeneration in Zantedeschia Hybrids.” Plant Cell Tissue and Organ Culture 87 (3): 329–331.
APA
Duquenne, B., Eeckhaut, T., Werbrouck, S., & Van Huylenbroeck, J. (2006). In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids. PLANT CELL TISSUE AND ORGAN CULTURE, 87(3), 329–331.
Vancouver
1.
Duquenne B, Eeckhaut T, Werbrouck S, Van Huylenbroeck J. In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids. PLANT CELL TISSUE AND ORGAN CULTURE. 2006;87(3):329–31.
MLA
Duquenne, Barbara, Tom Eeckhaut, Stefaan Werbrouck, et al. “In Vitro Somatic Embryogenesis and Plant Regeneration in Zantedeschia Hybrids.” PLANT CELL TISSUE AND ORGAN CULTURE 87.3 (2006): 329–331. Print.
@article{4276393,
  abstract     = {A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l(-1) in combination with 2 mg l(-1) alpha-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l(-1) 6-tau-tau-(dimethylallylamino)-purine (2iP), 3\% sucrose and 0.7\% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia.},
  author       = {Duquenne, Barbara and Eeckhaut, Tom and Werbrouck, Stefaan and Van Huylenbroeck, Johan},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {somatic embryo induction,tubers,callus formation,in vitro culture,Araceae},
  language     = {eng},
  number       = {3},
  pages        = {329--331},
  title        = {In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids},
  url          = {http://dx.doi.org/10.1007/s11240-006-9161-8},
  volume       = {87},
  year         = {2006},
}

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