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An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale

Sarah De Laethauwer UGent, Kathy Messens UGent, Veerle Derycke UGent, Nicolas Gryson, Dirk Reheul UGent and Geert Haesaert UGent (2006) Proceedings of the 6th international triticale symposium. p.39-44
abstract
An important role in tolerance to pre-harvest sprouting (phs) is attributed to the ‘viviparous’ VP1-orthologue. Conducting PCR, followed by restriction enzyme analysis, and RT-PCR on the Vp1-gene gave a strong indication that Vp1 is also present in triticale and rye, although slightly modified compared to wheat. Qualitative RT-PCR assays on mRNA of seeds under germination inducing conditions and sequencing of these Vp1-fragments revealed very little differences between phs sensitive and phs tolerant genotypes of triticale. However, differences in expression of Vp1 between phs sensitive and tolerant genotypes may be detected in a real-time quantitative RT-PCR assay, which can further be used in breeding programs to select for phs tolerant triticale genotypes.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
Proceedings of the 6th international triticale symposium
pages
39 - 44
publisher
Stellenbosch University
place of publication
Stellenbosch, South Africa
conference name
6th International Triticale symposium
conference location
Stellenbosch, South Africa
conference start
2006-09-03
conference end
2006-09-07
ISBN
9780620370080
language
English
UGent publication?
no
classification
C1
id
4274502
handle
http://hdl.handle.net/1854/LU-4274502
date created
2014-02-07 16:17:41
date last changed
2017-01-02 09:53:12
@inproceedings{4274502,
  abstract     = {An important role in tolerance to pre-harvest sprouting (phs) is attributed to the {\textquoteleft}viviparous{\textquoteright} VP1-orthologue. Conducting PCR, followed by restriction enzyme analysis, and RT-PCR on the Vp1-gene gave a strong indication that Vp1 is also present in triticale and rye, although slightly modified compared to wheat. Qualitative RT-PCR assays on mRNA of seeds under germination inducing conditions and sequencing of these Vp1-fragments revealed very little differences between phs sensitive and phs tolerant genotypes of triticale. However, differences in expression of Vp1 between phs sensitive and tolerant genotypes may be detected in a real-time quantitative RT-PCR assay, which can further be used in breeding programs to select for phs tolerant triticale genotypes.},
  author       = {De Laethauwer, Sarah and Messens, Kathy and Derycke, Veerle and Gryson, Nicolas and Reheul, Dirk and Haesaert, Geert},
  booktitle    = {Proceedings of the 6th international triticale symposium},
  isbn         = {9780620370080},
  language     = {eng},
  location     = {Stellenbosch, South Africa},
  pages        = {39--44},
  publisher    = {Stellenbosch University},
  title        = {An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale},
  year         = {2006},
}

Chicago
De Laethauwer, Sarah, Kathy Messens, Veerle Derycke, Nicolas Gryson, Dirk Reheul, and Geert Haesaert. 2006. “An Approach to Developing a Marker Assisted Selection System for Tolerance to Pre-harvest Sprouting in Triticale.” In Proceedings of the 6th International Triticale Symposium, 39–44. Stellenbosch, South Africa: Stellenbosch University.
APA
De Laethauwer, S., Messens, K., Derycke, V., Gryson, N., Reheul, D., & Haesaert, G. (2006). An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale. Proceedings of the 6th international triticale symposium (pp. 39–44). Presented at the 6th International Triticale symposium, Stellenbosch, South Africa: Stellenbosch University.
Vancouver
1.
De Laethauwer S, Messens K, Derycke V, Gryson N, Reheul D, Haesaert G. An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale. Proceedings of the 6th international triticale symposium. Stellenbosch, South Africa: Stellenbosch University; 2006. p. 39–44.
MLA
De Laethauwer, Sarah, Kathy Messens, Veerle Derycke, et al. “An Approach to Developing a Marker Assisted Selection System for Tolerance to Pre-harvest Sprouting in Triticale.” Proceedings of the 6th International Triticale Symposium. Stellenbosch, South Africa: Stellenbosch University, 2006. 39–44. Print.