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An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale

Sarah De Laethauwer (UGent) , Kathy Messens (UGent) , Veerle Derycke (UGent) , Nicolas Gryson (UGent) , Dirk Reheul (UGent) and Geert Haesaert (UGent)
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Abstract
An important role in tolerance to pre-harvest sprouting (phs) is attributed to the ‘viviparous’ VP1-orthologue. Conducting PCR, followed by restriction enzyme analysis, and RT-PCR on the Vp1-gene gave a strong indication that Vp1 is also present in triticale and rye, although slightly modified compared to wheat. Qualitative RT-PCR assays on mRNA of seeds under germination inducing conditions and sequencing of these Vp1-fragments revealed very little differences between phs sensitive and phs tolerant genotypes of triticale. However, differences in expression of Vp1 between phs sensitive and tolerant genotypes may be detected in a real-time quantitative RT-PCR assay, which can further be used in breeding programs to select for phs tolerant triticale genotypes.

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Chicago
De Laethauwer, Sarah, Kathy Messens, Veerle Derycke, Nicolas Gryson, Dirk Reheul, and Geert Haesaert. 2006. “An Approach to Developing a Marker Assisted Selection System for Tolerance to Pre-harvest Sprouting in Triticale.” In Proceedings of the 6th International Triticale Symposium, 39–44. Stellenbosch, South Africa: Stellenbosch University.
APA
De Laethauwer, S., Messens, K., Derycke, V., Gryson, N., Reheul, D., & Haesaert, G. (2006). An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale. Proceedings of the 6th international triticale symposium (pp. 39–44). Presented at the 6th International Triticale symposium, Stellenbosch, South Africa: Stellenbosch University.
Vancouver
1.
De Laethauwer S, Messens K, Derycke V, Gryson N, Reheul D, Haesaert G. An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale. Proceedings of the 6th international triticale symposium. Stellenbosch, South Africa: Stellenbosch University; 2006. p. 39–44.
MLA
De Laethauwer, Sarah, Kathy Messens, Veerle Derycke, et al. “An Approach to Developing a Marker Assisted Selection System for Tolerance to Pre-harvest Sprouting in Triticale.” Proceedings of the 6th International Triticale Symposium. Stellenbosch, South Africa: Stellenbosch University, 2006. 39–44. Print.
@inproceedings{4274502,
  abstract     = {An important role in tolerance to pre-harvest sprouting (phs) is attributed to the {\textquoteleft}viviparous{\textquoteright} VP1-orthologue. Conducting PCR, followed by restriction enzyme analysis, and RT-PCR on the Vp1-gene gave a strong indication that Vp1 is also present in triticale and rye, although slightly modified compared to wheat. Qualitative RT-PCR assays on mRNA of seeds under germination inducing conditions and sequencing of these Vp1-fragments revealed very little differences between phs sensitive and phs tolerant genotypes of triticale. However, differences in expression of Vp1 between phs sensitive and tolerant genotypes may be detected in a real-time quantitative RT-PCR assay, which can further be used in breeding programs to select for phs tolerant triticale genotypes.},
  author       = {De Laethauwer, Sarah and Messens, Kathy and Derycke, Veerle and Gryson, Nicolas and Reheul, Dirk and Haesaert, Geert},
  booktitle    = {Proceedings of the 6th international triticale symposium},
  isbn         = {9780620370080},
  language     = {eng},
  location     = {Stellenbosch, South Africa},
  pages        = {39--44},
  publisher    = {Stellenbosch University},
  title        = {An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in triticale},
  year         = {2006},
}