Advanced search
1 file | 214.04 KB

Androgenic and estrogenic regulation of Atrogin-1, MuRF1 and myostatin expression in different muscle types of male mice

Hélène De Naeyer (UGent) , S Lamon, A Russell, Inge Everaert (UGent) , Annelies De Spaey (UGent) , Bert Vanheel (UGent) , Youri Taes (UGent) and Wim Derave (UGent)
Author
Organization
Abstract
PURPOSE: The molecular factors targeted by androgens and estrogens on muscle mass are not fully understood. The current study aimed to explore gene and protein expression of Atrogin-1, MuRF1, and myostatin in an androgen deprivation-induced muscle atrophy model. METHODS: We examined the effects of Orx either with or without testosterone (T) or estradiol (E2) administration on Atrogin-1 gene expression, and MuRF1 and myostatin gene and protein expression. Measurements were made in soleus (SOL), extensor digitorum longus (EDL) and levator ani/bulbocavernosus (LA/BC) of male C57BL/6 mice. RESULTS: Thirty days of Orx resulted in a reduction in weight gain and muscle mass. These effects were prevented by T. In LA/BC, Atrogin-1 and MuRF1 mRNA was increased throughout 30 days of Orx, which was fully reversed by T and partially by E2 administration. In EDL and SOL, a less pronounced upregulation of both genes was only detectable at the early stages of Orx. Myostatin mRNA levels were downregulated in LA/BC and upregulated in EDL following Orx. T, but not E2, reversed these effects. No changes in protein levels of MuRF1 and myostatin were found in EDL at any time point following Orx. CONCLUSIONS: The atrophy in SOL and EDL in response to androgen deprivation, and its restoration by T, is accompanied by only minimal changes in atrogenes and myostatin gene expression. The marked differences in muscle atrophy and atrogene and myostatin mRNA between LA/BC and the locomotor muscles suggest that the murine LA/BC is not an optimal model to study Orx-induced muscle atrophy.
Keywords
MuRF1, Atrogin-1, Myostatin, Skeletal muscle atrophy, Androgens, HUMAN SKELETAL-MUSCLE, TESTOSTERONE-REPLACEMENT, GENE-EXPRESSION, RECEPTOR-ALPHA, UBIQUITIN LIGASE, FIBER-TYPE, MASS, MEN, ATROPHY, STRENGTH

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 214.04 KB

Citation

Please use this url to cite or link to this publication:

Chicago
De Naeyer, Hélène, S Lamon, A Russell, Inge Everaert, Annelies De Spaey, Bert Vanheel, Youri Taes, and Wim Derave. 2014. “Androgenic and Estrogenic Regulation of Atrogin-1, MuRF1 and Myostatin Expression in Different Muscle Types of Male Mice.” European Journal of Applied Physiology 114 (4): 751–761.
APA
De Naeyer, H., Lamon, S., Russell, A., Everaert, I., De Spaey, A., Vanheel, B., Taes, Y., et al. (2014). Androgenic and estrogenic regulation of Atrogin-1, MuRF1 and myostatin expression in different muscle types of male mice. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 114(4), 751–761.
Vancouver
1.
De Naeyer H, Lamon S, Russell A, Everaert I, De Spaey A, Vanheel B, et al. Androgenic and estrogenic regulation of Atrogin-1, MuRF1 and myostatin expression in different muscle types of male mice. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY. 2014;114(4):751–61.
MLA
De Naeyer, Hélène, S Lamon, A Russell, et al. “Androgenic and Estrogenic Regulation of Atrogin-1, MuRF1 and Myostatin Expression in Different Muscle Types of Male Mice.” EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY 114.4 (2014): 751–761. Print.
@article{4269745,
  abstract     = {PURPOSE: The molecular factors targeted by androgens and estrogens on muscle mass are not fully understood. The current study aimed to explore gene and protein expression of Atrogin-1, MuRF1, and myostatin in an androgen deprivation-induced muscle atrophy model.
METHODS: We examined the effects of Orx either with or without testosterone (T) or estradiol (E2) administration on Atrogin-1 gene expression, and MuRF1 and myostatin gene and protein expression. Measurements were made in soleus (SOL), extensor digitorum longus (EDL) and levator ani/bulbocavernosus (LA/BC) of male C57BL/6 mice.
RESULTS: Thirty days of Orx resulted in a reduction in weight gain and muscle mass. These effects were prevented by T. In LA/BC, Atrogin-1 and MuRF1 mRNA was increased throughout 30 days of Orx, which was fully reversed by T and partially by E2 administration. In EDL and SOL, a less pronounced upregulation of both genes was only detectable at the early stages of Orx. Myostatin mRNA levels were downregulated in LA/BC and upregulated in EDL following Orx. T, but not E2, reversed these effects. No changes in protein levels of MuRF1 and myostatin were found in EDL at any time point following Orx.
CONCLUSIONS: The atrophy in SOL and EDL in response to androgen deprivation, and its restoration by T, is accompanied by only minimal changes in atrogenes and myostatin gene expression. The marked differences in muscle atrophy and atrogene and myostatin mRNA between LA/BC and the locomotor muscles suggest that the murine LA/BC is not an optimal model to study Orx-induced muscle atrophy.},
  author       = {De Naeyer, H{\'e}l{\`e}ne and Lamon, S and Russell, A and Everaert, Inge and De Spaey, Annelies and Vanheel, Bert and Taes, Youri and Derave, Wim},
  issn         = {1439-6319},
  journal      = {EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY},
  language     = {eng},
  number       = {4},
  pages        = {751--761},
  title        = {Androgenic and estrogenic regulation of Atrogin-1, MuRF1 and myostatin expression in different muscle types of male mice},
  url          = {http://dx.doi.org/10.1007/s00421-013-2800-y},
  volume       = {114},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: