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Selective modulation of the glucocorticoid receptor can distinguish between transrepression of NF-κB and AP-1

Karolien De Bosscher UGent, Ilse Beck, Lien Dejager, Nadia Bougarne UGent, Anthoula Gaigneaux, Sebastien Chateauvieux, Dariusz Ratman, Marc Bracke UGent, Jan Tavernier UGent, Wim Vanden Berghe UGent, et al. (2014) CELLULAR AND MOLECULAR LIFE SCIENCES. 71(1). p.143-163
abstract
Glucocorticoids (GCs) block inflammation via interference of the liganded glucocorticoid receptor (GR) with the activity of pro-inflammatory transcription factors NF-kappa B and AP-1, a mechanism known as transrepression. This mechanism is believed to involve the activity of GR monomers. Here, we explored how the GR monomer-favoring Compound A (CpdA) affects AP-1 activation and activity. Our results demonstrate that non-steroidal CpdA, unlike classic steroidal GCs, blocks NF-kappa B- but not AP-1-driven gene expression. CpdA rather sustains AP-1-driven gene expression, a result which could mechanistically be explained by the failure of CpdA to block upstream JNK kinase activation and concomitantly also phosphorylation of c-Jun. In concordance and in contrast to DEX, CpdA maintained the expression of the activated AP-1 target gene c-jun, as well as the production of the c-Jun protein. As for the underlying mechanism, GR is a necessary intermediate in the CpdA-mediated gene expression of AP-1-regulated genes, but seems to be superfluous to CpdA-mediated JNK phosphorylation prolongation. The latter phenomenon concurs with the inability of CpdA to stimulate DUSP1 gene expression. ChIP analysis demonstrates that DEX-activated GR, but not CpdA-activated GR, is recruited to AP-1-driven promoters. Furthermore, in mice we observed that CpdA instigates a strong enhancement of TNF-induced AP-1-driven gene expression. Finally, we demonstrate that this phenomenon coincides with an increased sensitivity towards TNF lethality, and implicate again a role for JNK2. In conclusion, our data support the hypothesis that a ligand-induced differential conformation of GR yields a different transcription factor cross-talk profile.
Please use this url to cite or link to this publication:
author
organization
alternative title
Selective modulation of the glucocorticoid receptor can distinguish between transrepression of NF-kappa B and AP-1
year
type
journalArticle (original)
publication status
published
subject
keyword
C-JUN, TRANSCRIPTIONAL ACTIVATION, DNA-BINDING, JUN GENE-EXPRESSION, TUMOR-NECROSIS-FACTOR, Jun N-terminal kinase (JNK), c-Jun, Selective GR modulator, Mitogen-activated protein kinase (MAPK), Inflammation, Glucocorticoids, RHEUMATOID-ARTHRITIS, MOUSE MODEL, IN-VIVO, REPRESSION, TRANSACTIVATION
journal title
CELLULAR AND MOLECULAR LIFE SCIENCES
Cell. Mol. Life Sci.
volume
71
issue
1
pages
143 - 163
Web of Science type
Article
Web of Science id
000329223300007
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
5.808 (2014)
JCR rank
37/290 (2014)
JCR quartile
1 (2014)
ISSN
1420-682X
DOI
10.1007/s00018-013-1367-4
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4269524
handle
http://hdl.handle.net/1854/LU-4269524
date created
2014-02-07 09:08:11
date last changed
2016-12-19 15:44:53
@article{4269524,
  abstract     = {Glucocorticoids (GCs) block inflammation via interference of the liganded glucocorticoid receptor (GR) with the activity of pro-inflammatory transcription factors NF-kappa B and AP-1, a mechanism known as transrepression. This mechanism is believed to involve the activity of GR monomers. Here, we explored how the GR monomer-favoring Compound A (CpdA) affects AP-1 activation and activity. Our results demonstrate that non-steroidal CpdA, unlike classic steroidal GCs, blocks NF-kappa B- but not AP-1-driven gene expression. CpdA rather sustains AP-1-driven gene expression, a result which could mechanistically be explained by the failure of CpdA to block upstream JNK kinase activation and concomitantly also phosphorylation of c-Jun. In concordance and in contrast to DEX, CpdA maintained the expression of the activated AP-1 target gene c-jun, as well as the production of the c-Jun protein. As for the underlying mechanism, GR is a necessary intermediate in the CpdA-mediated gene expression of AP-1-regulated genes, but seems to be superfluous to CpdA-mediated JNK phosphorylation prolongation. The latter phenomenon concurs with the inability of CpdA to stimulate DUSP1 gene expression. ChIP analysis demonstrates that DEX-activated GR, but not CpdA-activated GR, is recruited to AP-1-driven promoters. Furthermore, in mice we observed that CpdA instigates a strong enhancement of TNF-induced AP-1-driven gene expression. Finally, we demonstrate that this phenomenon coincides with an increased sensitivity towards TNF lethality, and implicate again a role for JNK2. In conclusion, our data support the hypothesis that a ligand-induced differential conformation of GR yields a different transcription factor cross-talk profile.},
  author       = {De Bosscher, Karolien and Beck, Ilse and Dejager, Lien and Bougarne, Nadia and Gaigneaux, Anthoula and Chateauvieux, Sebastien and Ratman, Dariusz and Bracke, Marc and Tavernier, Jan and Vanden Berghe, Wim and Libert, Claude and Diederich, Marc and Haegeman, Guy},
  issn         = {1420-682X},
  journal      = {CELLULAR AND MOLECULAR LIFE SCIENCES},
  keyword      = {C-JUN,TRANSCRIPTIONAL ACTIVATION,DNA-BINDING,JUN GENE-EXPRESSION,TUMOR-NECROSIS-FACTOR,Jun N-terminal kinase (JNK),c-Jun,Selective GR modulator,Mitogen-activated protein kinase (MAPK),Inflammation,Glucocorticoids,RHEUMATOID-ARTHRITIS,MOUSE MODEL,IN-VIVO,REPRESSION,TRANSACTIVATION},
  language     = {eng},
  number       = {1},
  pages        = {143--163},
  title        = {Selective modulation of the glucocorticoid receptor can distinguish between transrepression of NF-\ensuremath{\kappa}B and AP-1},
  url          = {http://dx.doi.org/10.1007/s00018-013-1367-4},
  volume       = {71},
  year         = {2014},
}

Chicago
De Bosscher, Karolien, Ilse Beck, Lien Dejager, Nadia Bougarne, Anthoula Gaigneaux, Sebastien Chateauvieux, Dariusz Ratman, et al. 2014. “Selective Modulation of the Glucocorticoid Receptor Can Distinguish Between Transrepression of NF-κB and AP-1.” Cellular and Molecular Life Sciences 71 (1): 143–163.
APA
De Bosscher, K., Beck, I., Dejager, L., Bougarne, N., Gaigneaux, A., Chateauvieux, S., Ratman, D., et al. (2014). Selective modulation of the glucocorticoid receptor can distinguish between transrepression of NF-κB and AP-1. CELLULAR AND MOLECULAR LIFE SCIENCES, 71(1), 143–163.
Vancouver
1.
De Bosscher K, Beck I, Dejager L, Bougarne N, Gaigneaux A, Chateauvieux S, et al. Selective modulation of the glucocorticoid receptor can distinguish between transrepression of NF-κB and AP-1. CELLULAR AND MOLECULAR LIFE SCIENCES. 2014;71(1):143–63.
MLA
De Bosscher, Karolien, Ilse Beck, Lien Dejager, et al. “Selective Modulation of the Glucocorticoid Receptor Can Distinguish Between Transrepression of NF-κB and AP-1.” CELLULAR AND MOLECULAR LIFE SCIENCES 71.1 (2014): 143–163. Print.