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Detection of enterotoxin DNA in Staphylococcus aureus strains obtained from the middle meatus in controls and nasal polyp patients

(2008) AMERICAN JOURNAL OF RHINOLOGY. 22(3). p.223-227
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Abstract
Background: Recent findings indicate that Staphylococcus aureus and its products may be involved in the modification of nasal polyposis. The purpose of this study was to investigate the presence of S. aureus enterotoxin genes and the agr subtype in bacterial DNA of S. aureus strains isolated from the middle meatus of nasal polyp and control patients. Methods: S. aureus strains were isolated from nasal polyp patients and controls. The strains were screened using PCR for their agr subtype, classic superantigens (SEA, SEB, SEC, SED or TSST-1), the egc cluster (SEG, SEI, SEM, SEN, and SEO and other enterotoxins (SEE, SEH, SEJ, SEK, and SEL) distinct from the egc locus. Results: Seventy-five percent of S. aureus strains had at least one enterotoxin in their DNA. The egc gene cluster was identified in 27 (67.5%) strains. At least one classic enterotoxin gene was present in 42.5% of the strains. Interestingly, there were no differences in enterotoxin genes between S. aureus strains isolated from controls compared with nasal polyposis patients. In controls, an equal distribution among the four agr groups was found, while 73% of the NPs strains belonged to agr groups I and II. Conclusion: In this study we found no significant difference between strains from nasal polyp patients and controls in the presence of enterotoxin genes. However, in NPs, a higher number of strains belonged to agr I or II, which are associated with strains causing enterotoxin-mediated disease.
Keywords
EXOTOXINS, COLONIZATION, ALLELES, DISEASE, ATOPIC-DERMATITIS, IDENTIFICATION, SUPERANTIGENS, CHRONIC RHINOSINUSITIS, ENVIRONMENTAL-FACTORS, PCR, Staphylococcus aureus, superantigen, LENGTH POLYMORPHISM ANALYSIS, chronic rhinosinusitis, enterotoxin, nasal polyposis

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Chicago
Van Zele, Thibaut, Mario Vaneechoutte, Gabriële Holtappels, Philippe Gevaert, Paul Van Cauwenberge, and Claus Bachert. 2008. “Detection of Enterotoxin DNA in Staphylococcus Aureus Strains Obtained from the Middle Meatus in Controls and Nasal Polyp Patients.” American Journal of Rhinology 22 (3): 223–227.
APA
Van Zele, T., Vaneechoutte, M., Holtappels, G., Gevaert, P., Van Cauwenberge, P., & Bachert, C. (2008). Detection of enterotoxin DNA in Staphylococcus aureus strains obtained from the middle meatus in controls and nasal polyp patients. AMERICAN JOURNAL OF RHINOLOGY, 22(3), 223–227.
Vancouver
1.
Van Zele T, Vaneechoutte M, Holtappels G, Gevaert P, Van Cauwenberge P, Bachert C. Detection of enterotoxin DNA in Staphylococcus aureus strains obtained from the middle meatus in controls and nasal polyp patients. AMERICAN JOURNAL OF RHINOLOGY. 2008;22(3):223–7.
MLA
Van Zele, Thibaut, Mario Vaneechoutte, Gabriële Holtappels, et al. “Detection of Enterotoxin DNA in Staphylococcus Aureus Strains Obtained from the Middle Meatus in Controls and Nasal Polyp Patients.” AMERICAN JOURNAL OF RHINOLOGY 22.3 (2008): 223–227. Print.
@article{426935,
  abstract     = {Background: Recent findings indicate that Staphylococcus aureus and its products may be involved in the modification of nasal polyposis. The purpose of this study was to investigate the presence of S. aureus enterotoxin genes and the agr subtype in bacterial DNA of S. aureus strains isolated from the middle meatus of nasal polyp and control patients.
Methods: S. aureus strains were isolated from nasal polyp patients and controls. The strains were screened using PCR for their agr subtype, classic superantigens (SEA, SEB, SEC, SED or TSST-1), the egc cluster (SEG, SEI, SEM, SEN, and SEO and other enterotoxins (SEE, SEH, SEJ, SEK, and SEL) distinct from the egc locus.
Results: Seventy-five percent of S. aureus strains had at least one enterotoxin in their DNA. The egc gene cluster was identified in 27 (67.5\%) strains. At least one classic enterotoxin gene was present in 42.5\% of the strains. Interestingly, there were no differences in enterotoxin genes between S. aureus strains isolated from controls compared with nasal polyposis patients. In controls, an equal distribution among the four agr groups was found, while 73\% of the NPs strains belonged to agr groups I and II.
Conclusion: In this study we found no significant difference between strains from nasal polyp patients and controls in the presence of enterotoxin genes. However, in NPs, a higher number of strains belonged to agr I or II, which are associated with strains causing enterotoxin-mediated disease.},
  author       = {Van Zele, Thibaut and Vaneechoutte, Mario and Holtappels, Gabri{\"e}le and Gevaert, Philippe and Van Cauwenberge, Paul and Bachert, Claus},
  issn         = {1050-6586},
  journal      = {AMERICAN JOURNAL OF RHINOLOGY},
  keyword      = {EXOTOXINS,COLONIZATION,ALLELES,DISEASE,ATOPIC-DERMATITIS,IDENTIFICATION,SUPERANTIGENS,CHRONIC RHINOSINUSITIS,ENVIRONMENTAL-FACTORS,PCR,Staphylococcus aureus,superantigen,LENGTH POLYMORPHISM ANALYSIS,chronic rhinosinusitis,enterotoxin,nasal polyposis},
  language     = {eng},
  number       = {3},
  pages        = {223--227},
  title        = {Detection of enterotoxin DNA in Staphylococcus aureus strains obtained from the middle meatus in controls and nasal polyp patients},
  url          = {http://dx.doi.org/10.2500/ajr.2008.22.3161},
  volume       = {22},
  year         = {2008},
}

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