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Small-scale oxygen distribution determines the vinyl chloride biodegradation pathway in surficial sediments of riverbed hyporheic zones

(2013) FEMS MICROBIOLOGY ECOLOGY. 84(1). p.133-142
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Abstract
Surficial riverbed sediments are often characterized by sharp redox gradients between the aerobic benthic sediment and underlying anoxic sediment, potentially representing an ideal niche for aerobic and anaerobic vinyl chloride (VC) degraders. To test this, the fate of VC in aerobic and anaerobic microcosms containing surficial sediment of a riverbed hyporheic zone receiving VC-contaminated groundwater was explored. Quantitative PCR showed that Dehalococcoides 16S rRNA gene and VC reductive dehalogenaseencoding genes (vcrA, bvcA) were highly enriched in anaerobic microcosms, with stoichiometric conversion of VC to ethene. In aerobic microcosms, etnC and etnE involved in aerobic ethene/VC oxidation were enriched with concomitant low or no accumulation of ethene. However, Dehalococcoides 16S rRNA gene, vcrA and bvcA copy numbers were also enriched in oxygen-exposed microcosms containing sediment with high organic carbon and small grain size, whereas they were reduced in oxygen-exposed sediment with low organic carbon and larger grain size in line with extensive oxygen penetration into the sediment. These results suggest the coexistence and coactivity of anaerobic and aerobic VC degraders in the same small volume of surficial sediment and that oxygen distribution, as determined by sediment grain size and organic matter content, affects the local VC-degrading bacterial community and VC biodegradation pathway.
Keywords
reductive dechlorination, vinyl chloride, aerobic biodegradation, hyporheic zone, riverbed sediment, CHLORINATED ALIPHATIC-HYDROCARBONS, DEHALOCOCCOIDES SP STRAIN, CONTAMINATED GROUNDWATER, TIDAL FLAT, ETHENE, IDENTIFICATION, ATTENUATION, COMMUNITY, PERCHLOROETHENE, BIOREMEDIATION

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Chicago
Atashgahi, Siavash, Farai Maphosa, Eylem Dogan Subasi, Hauke Smidt, Dirk Springael, and Winnie Dejonghe. 2013. “Small-scale Oxygen Distribution Determines the Vinyl Chloride Biodegradation Pathway in Surficial Sediments of Riverbed Hyporheic Zones.” Fems Microbiology Ecology 84 (1): 133–142.
APA
Atashgahi, S., Maphosa, F., Dogan Subasi, E., Smidt, H., Springael, D., & Dejonghe, W. (2013). Small-scale oxygen distribution determines the vinyl chloride biodegradation pathway in surficial sediments of riverbed hyporheic zones. FEMS MICROBIOLOGY ECOLOGY, 84(1), 133–142.
Vancouver
1.
Atashgahi S, Maphosa F, Dogan Subasi E, Smidt H, Springael D, Dejonghe W. Small-scale oxygen distribution determines the vinyl chloride biodegradation pathway in surficial sediments of riverbed hyporheic zones. FEMS MICROBIOLOGY ECOLOGY. 2013;84(1):133–42.
MLA
Atashgahi, Siavash, Farai Maphosa, Eylem Dogan Subasi, et al. “Small-scale Oxygen Distribution Determines the Vinyl Chloride Biodegradation Pathway in Surficial Sediments of Riverbed Hyporheic Zones.” FEMS MICROBIOLOGY ECOLOGY 84.1 (2013): 133–142. Print.
@article{4269079,
  abstract     = {Surficial riverbed sediments are often characterized by sharp redox gradients between the aerobic benthic sediment and underlying anoxic sediment, potentially representing an ideal niche for aerobic and anaerobic vinyl chloride (VC) degraders. To test this, the fate of VC in aerobic and anaerobic microcosms containing surficial sediment of a riverbed hyporheic zone receiving VC-contaminated groundwater was explored. Quantitative PCR showed that Dehalococcoides 16S rRNA gene and VC reductive dehalogenaseencoding genes (vcrA, bvcA) were highly enriched in anaerobic microcosms, with stoichiometric conversion of VC to ethene. In aerobic microcosms, etnC and etnE involved in aerobic ethene/VC oxidation were enriched with concomitant low or no accumulation of ethene. However, Dehalococcoides 16S rRNA gene, vcrA and bvcA copy numbers were also enriched in oxygen-exposed microcosms containing sediment with high organic carbon and small grain size, whereas they were reduced in oxygen-exposed sediment with low organic carbon and larger grain size in line with extensive oxygen penetration into the sediment. These results suggest the coexistence and coactivity of anaerobic and aerobic VC degraders in the same small volume of surficial sediment and that oxygen distribution, as determined by sediment grain size and organic matter content, affects the local VC-degrading bacterial community and VC biodegradation pathway.},
  author       = {Atashgahi, Siavash and Maphosa, Farai and Dogan Subasi, Eylem and Smidt, Hauke and Springael, Dirk  and Dejonghe, Winnie},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keyword      = {reductive dechlorination,vinyl chloride,aerobic biodegradation,hyporheic zone,riverbed sediment,CHLORINATED ALIPHATIC-HYDROCARBONS,DEHALOCOCCOIDES SP STRAIN,CONTAMINATED GROUNDWATER,TIDAL FLAT,ETHENE,IDENTIFICATION,ATTENUATION,COMMUNITY,PERCHLOROETHENE,BIOREMEDIATION},
  language     = {eng},
  number       = {1},
  pages        = {133--142},
  title        = {Small-scale oxygen distribution determines the vinyl chloride biodegradation pathway in surficial sediments of riverbed hyporheic zones},
  url          = {http://dx.doi.org/10.1111/1574-6941.12044},
  volume       = {84},
  year         = {2013},
}

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