Advanced search
1 file | 3.00 MB

Gut microbiota affects sensitivity to acute DSS-induced colitis independently of host genotype

(2013) INFLAMMATORY BOWEL DISEASES. 19(12). p.2560-2567
Author
Organization
Abstract
Caspase-deficient mice and wild-type (WT) mice show significant differences in their gut microbiota composition. These differences coincide with the observation that caspase-3-deficient mice carrying a natural caspase-11 mutation (Casp3/11(-/-)) are less sensitive to acute dextran sodium sulfate-induced colitis than WT mice. For these reasons, we investigated the role of the microbiota in the development of colitis by cohousing WT and Casp3/11(-/-) mice. Microbial community fingerprinting by denaturing gradient gel electrophoresis analysis revealed that the similarities in gut microbial composition of WT and Casp3/11(-/-) mice increased after cohousing. In the acute dextran sodium sulfate-induced colitis model, Casp3/11(-/-) mice that were cohoused with WT mice showed increased weight loss and disease activity scores and increased neutrophil infiltration and inflammatory cytokine levels in their colon tissue compared with Casp3/11(-/-) mice that were not cohoused with WT mice. Also, we demonstrate that only the microbiota of the Casp3/11(-/-) mice cohoused with WT mice showed an important increase in Prevotella species. In conclusion, our cohousing experiments revealed that the colitogenic activity of the WT microbiota is transferable to Casp3/11(-/-) mice and that Prevotella species are likely to be involved. By contrast, the relative protection of Casp3/11(-/-) mice against dextran sodium sulfate damage is not transferred to WT mice after cohousing. These results underscore the need for in-depth studies of the bilateral interaction of host genes and microbiota to gain insight into the mechanisms of disease pathogenesis. Our findings also have important implications for the experimental design of disease studies in genetically modified mice and conclusions drawn from them.
Keywords
caspase, GRADIENT GEL-ELECTROPHORESIS, knockout, DSS, gut microbiota, QUANTITATIVE PCR, PATHOGENESIS, LABORATORY MICE, LESSONS, DYSBIOSIS, colitis, INFLAMMATORY-BOWEL-DISEASE, cohousing, ULCERATIVE-COLITIS, OBESITY, BACTEROIDES, DGGE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.00 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Brinkman, Brigitta, Anne Becker, Rene Ayiseh, Falk Hildebrand, Jeroen Raes, Geert Huys, and Peter Vandenabeele. 2013. “Gut Microbiota Affects Sensitivity to Acute DSS-induced Colitis Independently of Host Genotype.” Inflammatory Bowel Diseases 19 (12): 2560–2567.
APA
Brinkman, B., Becker, A., Ayiseh, R., Hildebrand, F., Raes, J., Huys, G., & Vandenabeele, P. (2013). Gut microbiota affects sensitivity to acute DSS-induced colitis independently of host genotype. INFLAMMATORY BOWEL DISEASES, 19(12), 2560–2567.
Vancouver
1.
Brinkman B, Becker A, Ayiseh R, Hildebrand F, Raes J, Huys G, et al. Gut microbiota affects sensitivity to acute DSS-induced colitis independently of host genotype. INFLAMMATORY BOWEL DISEASES. 2013;19(12):2560–7.
MLA
Brinkman, Brigitta, Anne Becker, Rene Ayiseh, et al. “Gut Microbiota Affects Sensitivity to Acute DSS-induced Colitis Independently of Host Genotype.” INFLAMMATORY BOWEL DISEASES 19.12 (2013): 2560–2567. Print.
@article{4265968,
  abstract     = {Caspase-deficient mice and wild-type (WT) mice show significant differences in their gut microbiota composition. These differences coincide with the observation that caspase-3-deficient mice carrying a natural caspase-11 mutation (Casp3/11(-/-)) are less sensitive to acute dextran sodium sulfate-induced colitis than WT mice. For these reasons, we investigated the role of the microbiota in the development of colitis by cohousing WT and Casp3/11(-/-) mice. Microbial community fingerprinting by denaturing gradient gel electrophoresis analysis revealed that the similarities in gut microbial composition of WT and Casp3/11(-/-) mice increased after cohousing. In the acute dextran sodium sulfate-induced colitis model, Casp3/11(-/-) mice that were cohoused with WT mice showed increased weight loss and disease activity scores and increased neutrophil infiltration and inflammatory cytokine levels in their colon tissue compared with Casp3/11(-/-) mice that were not cohoused with WT mice. Also, we demonstrate that only the microbiota of the Casp3/11(-/-) mice cohoused with WT mice showed an important increase in Prevotella species. In conclusion, our cohousing experiments revealed that the colitogenic activity of the WT microbiota is transferable to Casp3/11(-/-) mice and that Prevotella species are likely to be involved. By contrast, the relative protection of Casp3/11(-/-) mice against dextran sodium sulfate damage is not transferred to WT mice after cohousing. These results underscore the need for in-depth studies of the bilateral interaction of host genes and microbiota to gain insight into the mechanisms of disease pathogenesis. Our findings also have important implications for the experimental design of disease studies in genetically modified mice and conclusions drawn from them.},
  author       = {Brinkman, Brigitta and Becker, Anne and Ayiseh, Rene and Hildebrand, Falk and Raes, Jeroen and Huys, Geert and Vandenabeele, Peter},
  issn         = {1078-0998},
  journal      = {INFLAMMATORY BOWEL DISEASES},
  keywords     = {caspase,GRADIENT GEL-ELECTROPHORESIS,knockout,DSS,gut microbiota,QUANTITATIVE PCR,PATHOGENESIS,LABORATORY MICE,LESSONS,DYSBIOSIS,colitis,INFLAMMATORY-BOWEL-DISEASE,cohousing,ULCERATIVE-COLITIS,OBESITY,BACTEROIDES,DGGE},
  language     = {eng},
  number       = {12},
  pages        = {2560--2567},
  title        = {Gut microbiota affects sensitivity to acute DSS-induced colitis independently of host genotype},
  url          = {http://dx.doi.org/10.1097/MIB.0b013e3182a8759a},
  volume       = {19},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: