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A combination of synchrotron and laboratory X-ray techniques for studying tissue-specific trace level metal distributions in Daphnia magna

Björn De Samber (UGent) , Roel Evens (UGent) , Karel De Schamphelaere (UGent) , Geert Silversmit (UGent) , Bert Masschaele (UGent) , Tom Schoonjans (UGent) , Bart Vekemans (UGent) , Colin Janssen (UGent) , Luc Van Hoorebeke (UGent) , I Szalóki, et al.
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Abstract
In the field of environmental toxicology, the study on the effects of the presence of transition metals such as Cu, Ni and Zn on the health of pelagic and benthic invertebrates is an important research topic. Fast dynamic 2D micro-XRF scanning and dynamic XRF micro-CT experiments on D. magna, a frequently used ecotoxicological model organism, allow the quantitative investigation of the accumulation of metals within specific organs with microscopic resolution. K-means clustering allows comparison of the concentrations of the elements of interest between different tissues/organs. Principal component analysis allows most of the significant statistical variations in these large and complex multivariate data sets to be explained. Also, by combining SR-XRF and absorption CT data sets using appropriate software packages, it becomes possible to unravel the tissue-specific 2D/3D distribution of metals in-situ within delicate organic samples on the 3-15 mu m resolution level in an essentially non-destructive manner.
Keywords
SPECTRA, ION, WATER, ZINC, TOXICITY, FLUORESCENCE, BIOTIC LIGAND MODEL, MICRO-XRF

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Chicago
De Samber, Björn, Roel Evens, Karel De Schamphelaere, Geert Silversmit, Bert Masschaele, Tom Schoonjans, Bart Vekemans, et al. 2008. “A Combination of Synchrotron and Laboratory X-ray Techniques for Studying Tissue-specific Trace Level Metal Distributions in Daphnia Magna.” Journal of Analytical Atomic Spectrometry 23 (6): 829–839.
APA
De Samber, B., Evens, R., De Schamphelaere, K., Silversmit, G., Masschaele, B., Schoonjans, T., Vekemans, B., et al. (2008). A combination of synchrotron and laboratory X-ray techniques for studying tissue-specific trace level metal distributions in Daphnia magna. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 23(6), 829–839.
Vancouver
1.
De Samber B, Evens R, De Schamphelaere K, Silversmit G, Masschaele B, Schoonjans T, et al. A combination of synchrotron and laboratory X-ray techniques for studying tissue-specific trace level metal distributions in Daphnia magna. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 2008;23(6):829–39.
MLA
De Samber, Björn, Roel Evens, Karel De Schamphelaere, et al. “A Combination of Synchrotron and Laboratory X-ray Techniques for Studying Tissue-specific Trace Level Metal Distributions in Daphnia Magna.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 23.6 (2008): 829–839. Print.
@article{426361,
  abstract     = {In the field of environmental toxicology, the study on the effects of the presence of transition metals such as Cu, Ni and Zn on the health of pelagic and benthic invertebrates is an important research topic. Fast dynamic 2D micro-XRF scanning and dynamic XRF micro-CT experiments on D. magna, a frequently used ecotoxicological model organism, allow the quantitative investigation of the accumulation of metals within specific organs with microscopic resolution. K-means clustering allows comparison of the concentrations of the elements of interest between different tissues/organs. Principal component analysis allows most of the significant statistical variations in these large and complex multivariate data sets to be explained. Also, by combining SR-XRF and absorption CT data sets using appropriate software packages, it becomes possible to unravel the tissue-specific 2D/3D distribution of metals in-situ within delicate organic samples on the 3-15 mu m resolution level in an essentially non-destructive manner.},
  author       = {De Samber, Bj{\"o}rn and Evens, Roel and De Schamphelaere, Karel and Silversmit, Geert and Masschaele, Bert and Schoonjans, Tom and Vekemans, Bart and Janssen, Colin and Van Hoorebeke, Luc and Szal{\'o}ki, I and Vanhaecke, Frank and Falkenberg, G and Vincze, Laszlo},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {SPECTRA,ION,WATER,ZINC,TOXICITY,FLUORESCENCE,BIOTIC LIGAND MODEL,MICRO-XRF},
  language     = {eng},
  number       = {6},
  pages        = {829--839},
  title        = {A combination of synchrotron and laboratory X-ray techniques for studying tissue-specific trace level metal distributions in Daphnia magna},
  url          = {http://dx.doi.org/10.1039/b800343m},
  volume       = {23},
  year         = {2008},
}

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