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Isolation of protoplasts from callus of Maesa species

Ellen Lambert (UGent) and Danny Geelen (UGent)
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Abstract
Maesa species are used in traditional medicine in many African and Asian countries. Maesa plants could, however, also play a role in modern medicine, for example in the cure of cancer or Leishmania. Combination of the genome of different Maesa species may result in a change in the secondary metabolism. Protoplast fusion is one possible strategy for making Maesa hybrids. The aim of this study was to isolate protoplasts from callus material of Maesa species. We found that the optimal enzyme mixture consisted of 1% Cellulase R-10, 0.5% Macerozyme R-10 and 0.6 M mannitol. Using this enzyme mixture we were able to isolate 7.7 x 10(5) protoplasts g(-1) fresh material for Maesa perlarius, 5.0 x 10(5) protoplasts g(-1) fresh material for Maesa balansae and 4.2 x 10(5) protoplasts g(-1) fresh material for Maesa lanceolata. In addition, a new method for automatically counting protoplasts was introduced.
Keywords
secondary metabolism, saponins, callus induction, protoplast isolation, Multisizer, SOMATIC HYBRIDIZATION, TRITERPENOID SAPONINS, BIOLOGICAL-ACTIVITIES, DISEASE RESISTANCE, SOLANUM-TUBEROSUM, LANCEOLATA, EXTRACT, HYBRIDS, PLANTS, REGENERATION

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MLA
Lambert, Ellen, and Danny Geelen. “Isolation of Protoplasts from Callus of Maesa Species.” Acta Horticulturae. Ed. D Baricevic, J Novak, & F Pank. Vol. 860. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2010. 241–248. Print.
APA
Lambert, Ellen, & Geelen, D. (2010). Isolation of protoplasts from callus of Maesa species. In D. Baricevic, J. Novak, & F. Pank (Eds.), Acta Horticulturae (Vol. 860, pp. 241–248). Presented at the 4th International symposium on Breeding Research on Medicinal and Aromatic Plants (ISBMAP 2009), Leuven, Belgium: International Society for Horticultural Science (ISHS).
Chicago author-date
Lambert, Ellen, and Danny Geelen. 2010. “Isolation of Protoplasts from Callus of Maesa Species.” In Acta Horticulturae, ed. D Baricevic, J Novak, and F Pank, 860:241–248. Leuven, Belgium: International Society for Horticultural Science (ISHS).
Chicago author-date (all authors)
Lambert, Ellen, and Danny Geelen. 2010. “Isolation of Protoplasts from Callus of Maesa Species.” In Acta Horticulturae, ed. D Baricevic, J Novak, and F Pank, 860:241–248. Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Lambert E, Geelen D. Isolation of protoplasts from callus of Maesa species. In: Baricevic D, Novak J, Pank F, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2010. p. 241–8.
IEEE
[1]
E. Lambert and D. Geelen, “Isolation of protoplasts from callus of Maesa species,” in Acta Horticulturae, Ljubljana, Slovenia, 2010, vol. 860, pp. 241–248.
@inproceedings{4261315,
  abstract     = {Maesa species are used in traditional medicine in many African and Asian countries. Maesa plants could, however, also play a role in modern medicine, for example in the cure of cancer or Leishmania. Combination of the genome of different Maesa species may result in a change in the secondary metabolism. Protoplast fusion is one possible strategy for making Maesa hybrids. The aim of this study was to isolate protoplasts from callus material of Maesa species. We found that the optimal enzyme mixture consisted of 1% Cellulase R-10, 0.5% Macerozyme R-10 and 0.6 M mannitol. Using this enzyme mixture we were able to isolate 7.7 x 10(5) protoplasts g(-1) fresh material for Maesa perlarius, 5.0 x 10(5) protoplasts g(-1) fresh material for Maesa balansae and 4.2 x 10(5) protoplasts g(-1) fresh material for Maesa lanceolata. In addition, a new method for automatically counting protoplasts was introduced.},
  author       = {Lambert, Ellen and Geelen, Danny},
  booktitle    = {Acta Horticulturae},
  editor       = {Baricevic, D and Novak, J and Pank, F},
  isbn         = {9789066052703},
  issn         = {0567-7572},
  keywords     = {secondary metabolism,saponins,callus induction,protoplast isolation,Multisizer,SOMATIC HYBRIDIZATION,TRITERPENOID SAPONINS,BIOLOGICAL-ACTIVITIES,DISEASE RESISTANCE,SOLANUM-TUBEROSUM,LANCEOLATA,EXTRACT,HYBRIDS,PLANTS,REGENERATION},
  language     = {eng},
  location     = {Ljubljana, Slovenia},
  pages        = {241--248},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Isolation of protoplasts from callus of Maesa species},
  url          = {http://www.actahort.org/books/860/860_36.htm},
  volume       = {860},
  year         = {2010},
}

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