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Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways

(2014) BLOOD. 123(5). p.e1-e10
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
One of the most important physiological platelet inhibitors is endothelium-derived prostacyclin which stimulates the platelet cyclic adenosine monophosphate/protein kinase A (cAMP/PKA)-signaling cascade and inhibits virtually all platelet-activating key mechanisms. Using quantitative mass spectrometry, we analyzed time-resolved phosphorylation patterns in human platelets after treatment with iloprost, a stable prostacyclin analog, for 0, 10, 30, and 60 seconds to characterize key mediators of platelet inhibition and activation in 3 independent biological replicates. We quantified over 2700 different phosphorylated peptides of which 360 were significantly regulated upon stimulation. This comprehensive and time-resolved analysis indicates that platelet inhibition is a multi-pronged process involving different kinases and phosphatases as well as many previously unanticipated proteins and pathways.
Keywords
THROMBOXANE A(2) RECEPTOR, PROTEIN-KINASE-C, PHOSPHORYLATION SITES, ALPHA, ACTIVATION, DESENSITIZATION, PROSTACYCLIN, AGGREGATION, UBIQUITINATION, SPECIFICITY

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Citation

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MLA
Beck, Florian, Jörg Geiger, Stepan Gambaryan, et al. “Time-resolved Characterization of cAMP/PKA-dependent Signaling Reveals That Platelet Inhibition Is a Concerted Process Involving Multiple Signaling Pathways.” BLOOD 123.5 (2014): e1–e10. Print.
APA
Beck, F., Geiger, J., Gambaryan, S., Veit, J., Vaudel, M., Nollau, P., Kohlbacher, O., et al. (2014). Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. BLOOD, 123(5), e1–e10.
Chicago author-date
Beck, Florian, Jörg Geiger, Stepan Gambaryan, Johannes Veit, Marc Vaudel, Peter Nollau, Oliver Kohlbacher, et al. 2014. “Time-resolved Characterization of cAMP/PKA-dependent Signaling Reveals That Platelet Inhibition Is a Concerted Process Involving Multiple Signaling Pathways.” Blood 123 (5): e1–e10.
Chicago author-date (all authors)
Beck, Florian, Jörg Geiger, Stepan Gambaryan, Johannes Veit, Marc Vaudel, Peter Nollau, Oliver Kohlbacher, Lennart Martens, Ulrich Walter, Albert Sickmann, and René P Zahedi. 2014. “Time-resolved Characterization of cAMP/PKA-dependent Signaling Reveals That Platelet Inhibition Is a Concerted Process Involving Multiple Signaling Pathways.” Blood 123 (5): e1–e10.
Vancouver
1.
Beck F, Geiger J, Gambaryan S, Veit J, Vaudel M, Nollau P, et al. Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. BLOOD. 2014;123(5):e1–e10.
IEEE
[1]
F. Beck et al., “Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways,” BLOOD, vol. 123, no. 5, pp. e1–e10, 2014.
@article{4259731,
  abstract     = {One of the most important physiological platelet inhibitors is endothelium-derived prostacyclin which stimulates the platelet cyclic adenosine monophosphate/protein kinase A (cAMP/PKA)-signaling cascade and inhibits virtually all platelet-activating key mechanisms. Using quantitative mass spectrometry, we analyzed time-resolved phosphorylation patterns in human platelets after treatment with iloprost, a stable prostacyclin analog, for 0, 10, 30, and 60 seconds to characterize key mediators of platelet inhibition and activation in 3 independent biological replicates. We quantified over 2700 different phosphorylated peptides of which 360 were significantly regulated upon stimulation. This comprehensive and time-resolved analysis indicates that platelet inhibition is a multi-pronged process involving different kinases and phosphatases as well as many previously unanticipated proteins and pathways.},
  author       = {Beck, Florian and Geiger, Jörg and Gambaryan, Stepan and Veit, Johannes and Vaudel, Marc and Nollau, Peter and Kohlbacher, Oliver and Martens, Lennart and Walter, Ulrich and Sickmann, Albert and Zahedi, René P},
  issn         = {0006-4971},
  journal      = {BLOOD},
  keywords     = {THROMBOXANE A(2) RECEPTOR,PROTEIN-KINASE-C,PHOSPHORYLATION SITES,ALPHA,ACTIVATION,DESENSITIZATION,PROSTACYCLIN,AGGREGATION,UBIQUITINATION,SPECIFICITY},
  language     = {eng},
  number       = {5},
  pages        = {e1--e10},
  title        = {Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways},
  url          = {http://dx.doi.org/10.1182/blood-2013-07-512384},
  volume       = {123},
  year         = {2014},
}

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