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Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways

(2014) BLOOD. 123(5). p.e1-e10
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
One of the most important physiological platelet inhibitors is endothelium-derived prostacyclin which stimulates the platelet cyclic adenosine monophosphate/protein kinase A (cAMP/PKA)-signaling cascade and inhibits virtually all platelet-activating key mechanisms. Using quantitative mass spectrometry, we analyzed time-resolved phosphorylation patterns in human platelets after treatment with iloprost, a stable prostacyclin analog, for 0, 10, 30, and 60 seconds to characterize key mediators of platelet inhibition and activation in 3 independent biological replicates. We quantified over 2700 different phosphorylated peptides of which 360 were significantly regulated upon stimulation. This comprehensive and time-resolved analysis indicates that platelet inhibition is a multi-pronged process involving different kinases and phosphatases as well as many previously unanticipated proteins and pathways.
Keywords
THROMBOXANE A(2) RECEPTOR, PROTEIN-KINASE-C, PHOSPHORYLATION SITES, ALPHA, ACTIVATION, DESENSITIZATION, PROSTACYCLIN, AGGREGATION, UBIQUITINATION, SPECIFICITY

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Citation

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Chicago
Beck, Florian, Jörg Geiger, Stepan Gambaryan, Johannes Veit, Marc Vaudel, Peter Nollau, Oliver Kohlbacher, et al. 2014. “Time-resolved Characterization of cAMP/PKA-dependent Signaling Reveals That Platelet Inhibition Is a Concerted Process Involving Multiple Signaling Pathways.” Blood 123 (5): e1–e10.
APA
Beck, F., Geiger, J., Gambaryan, S., Veit, J., Vaudel, M., Nollau, P., Kohlbacher, O., et al. (2014). Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. BLOOD, 123(5), e1–e10.
Vancouver
1.
Beck F, Geiger J, Gambaryan S, Veit J, Vaudel M, Nollau P, et al. Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. BLOOD. 2014;123(5):e1–e10.
MLA
Beck, Florian, Jörg Geiger, Stepan Gambaryan, et al. “Time-resolved Characterization of cAMP/PKA-dependent Signaling Reveals That Platelet Inhibition Is a Concerted Process Involving Multiple Signaling Pathways.” BLOOD 123.5 (2014): e1–e10. Print.
@article{4259731,
  abstract     = {One of the most important physiological platelet inhibitors is endothelium-derived prostacyclin which stimulates the platelet cyclic adenosine monophosphate/protein kinase A (cAMP/PKA)-signaling cascade and inhibits virtually all platelet-activating key mechanisms. Using quantitative mass spectrometry, we analyzed time-resolved phosphorylation patterns in human platelets after treatment with iloprost, a stable prostacyclin analog, for 0, 10, 30, and 60 seconds to characterize key mediators of platelet inhibition and activation in 3 independent biological replicates. We quantified over 2700 different phosphorylated peptides of which 360 were significantly regulated upon stimulation. This comprehensive and time-resolved analysis indicates that platelet inhibition is a multi-pronged process involving different kinases and phosphatases as well as many previously unanticipated proteins and pathways.},
  author       = {Beck, Florian and Geiger, J{\"o}rg and Gambaryan, Stepan and Veit, Johannes and Vaudel, Marc and Nollau, Peter and Kohlbacher, Oliver and Martens, Lennart and Walter, Ulrich and Sickmann, Albert and Zahedi, Ren{\'e} P},
  issn         = {0006-4971},
  journal      = {BLOOD},
  keyword      = {THROMBOXANE A(2) RECEPTOR,PROTEIN-KINASE-C,PHOSPHORYLATION SITES,ALPHA,ACTIVATION,DESENSITIZATION,PROSTACYCLIN,AGGREGATION,UBIQUITINATION,SPECIFICITY},
  language     = {eng},
  number       = {5},
  pages        = {e1--e10},
  title        = {Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways},
  url          = {http://dx.doi.org/10.1182/blood-2013-07-512384},
  volume       = {123},
  year         = {2014},
}

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