Advanced search
1 file | 949.24 KB Add to list

Induction and disappearance of γH2AX foci and formation of micronuclei after exposure of human lymphocytes to 60Co γ-rays and p(66)+Be(40) neutrons

Author
Organization
Abstract
Purpose: To investigate both the formation of micronuclei (MN) and the induction and subsequent loss of phosphorylated histone H2AX foci (γH2AX foci) after in vitro exposure of human lymphocytes to either 60Co γ-rays or p(66)+Be(40) neutrons. Materials and methods: MN dose response (DR) curves were obtained by exposing isolated lymphocytes of 10 different donors to doses ranging from 0 to 4 Gy γ-rays or 0 to 2 Gy neutrons. Also, γH2AX foci DR curves were obtained following exposure to doses ranging from 0 to 0.5 Gy of either γ-rays or neutrons. Foci kinetics for lymphocytes for a single donor exposed to 0.5 Gy γ-rays or neutrons were studied up to 24 hours post-irradiation. Results: Micronuclei yields following neutron exposure were consistently higher compared to that from 60Co γ-rays. All MN yields were over-dispersed compared to a Poisson distribution. Over-dispersion was higher after neutron irradiation for all doses > 0.1 Gy. Up to 4 hours post-irradiation lower yields of neutron-induced γH2AX foci were observed. Between 4 and 24 hours the numbers of foci from neutrons were consistently higher than that from γ-rays. The half-live of foci disappearance is only marginally longer for neutrons compared to that from γ-rays. Foci formations were more likely to be over-dispersed for neutron irradiations. Conclusion: Although neutrons are more effective to induce MN, the absolute number of induced γH2AX foci are less at first compared to γ-rays. With time neutron-induced foci are more persistent. These findings are helpful for using γH2AX foci in biodosimetry and to understand the repair of neutron-induced cellular damage.
Keywords
gamma H2AX foci, Micronucleus, neutrons, DOUBLE-STRAND BREAKS, RELATIVE BIOLOGICAL EFFECTIVENESS, IONIZING-RADIATION, HUMAN-CELLS, DNA-DAMAGE, HIGH-LET, IN-VIVO, CHROMOSOME-ABERRATIONS, KU70/80 KNOCKDOWN, CHROMATIN DOMAINS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 949.24 KB

Citation

Please use this url to cite or link to this publication:

MLA
Vandersickel, Veerle, Philip Beukes, Bram Van Bockstaele, et al. “Induction and Disappearance of γH2AX Foci and Formation of Micronuclei After Exposure of Human Lymphocytes to 60Co Γ-rays and p(66)+Be(40) Neutrons.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 90.2 (2014): 149–158. Print.
APA
Vandersickel, V., Beukes, P., Van Bockstaele, B., Depuydt, J., Vral, A., & Slabbert, J. (2014). Induction and disappearance of γH2AX foci and formation of micronuclei after exposure of human lymphocytes to 60Co γ-rays and p(66)+Be(40) neutrons. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 90(2), 149–158.
Chicago author-date
Vandersickel, Veerle, Philip Beukes, Bram Van Bockstaele, Julie Depuydt, Anne Vral, and Jacobus Slabbert. 2014. “Induction and Disappearance of γH2AX Foci and Formation of Micronuclei After Exposure of Human Lymphocytes to 60Co Γ-rays and p(66)+Be(40) Neutrons.” International Journal of Radiation Biology 90 (2): 149–158.
Chicago author-date (all authors)
Vandersickel, Veerle, Philip Beukes, Bram Van Bockstaele, Julie Depuydt, Anne Vral, and Jacobus Slabbert. 2014. “Induction and Disappearance of γH2AX Foci and Formation of Micronuclei After Exposure of Human Lymphocytes to 60Co Γ-rays and p(66)+Be(40) Neutrons.” International Journal of Radiation Biology 90 (2): 149–158.
Vancouver
1.
Vandersickel V, Beukes P, Van Bockstaele B, Depuydt J, Vral A, Slabbert J. Induction and disappearance of γH2AX foci and formation of micronuclei after exposure of human lymphocytes to 60Co γ-rays and p(66)+Be(40) neutrons. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2014;90(2):149–58.
IEEE
[1]
V. Vandersickel, P. Beukes, B. Van Bockstaele, J. Depuydt, A. Vral, and J. Slabbert, “Induction and disappearance of γH2AX foci and formation of micronuclei after exposure of human lymphocytes to 60Co γ-rays and p(66)+Be(40) neutrons,” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, vol. 90, no. 2, pp. 149–158, 2014.
@article{4259156,
  abstract     = {Purpose: To investigate both the formation of micronuclei (MN) and the induction and subsequent loss of phosphorylated histone H2AX foci (γH2AX foci) after in vitro exposure of human lymphocytes to either 60Co γ-rays or p(66)+Be(40) neutrons.
Materials and methods: MN dose response (DR) curves were obtained by exposing isolated lymphocytes of 10 different donors to doses ranging from 0 to 4 Gy γ-rays or 0 to 2 Gy neutrons. Also, γH2AX foci DR curves were obtained following exposure to doses ranging from 0 to 0.5 Gy of either γ-rays or neutrons. Foci kinetics for lymphocytes for a single donor exposed to 0.5 Gy γ-rays or neutrons were studied up to 24 hours post-irradiation.
Results: Micronuclei yields following neutron exposure were consistently higher compared to that from 60Co γ-rays. All MN yields were over-dispersed compared to a Poisson distribution. Over-dispersion was higher after neutron irradiation for all doses > 0.1 Gy. Up to 4 hours post-irradiation lower yields of neutron-induced γH2AX foci were observed. Between 4 and 24 hours the numbers of foci from neutrons were consistently higher than that from γ-rays. The half-live of foci disappearance is only marginally longer for neutrons compared to that from γ-rays. Foci formations were more likely to be over-dispersed for neutron irradiations.
Conclusion: Although neutrons are more effective to induce MN, the absolute number of induced γH2AX foci are less at first compared to γ-rays. With time neutron-induced foci are more persistent. These findings are helpful for using γH2AX foci in biodosimetry and to understand the repair of neutron-induced cellular damage.},
  author       = {Vandersickel, Veerle and Beukes, Philip and Van Bockstaele, Bram and Depuydt, Julie and Vral, Anne and Slabbert, Jacobus},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keywords     = {gamma H2AX foci,Micronucleus,neutrons,DOUBLE-STRAND BREAKS,RELATIVE BIOLOGICAL EFFECTIVENESS,IONIZING-RADIATION,HUMAN-CELLS,DNA-DAMAGE,HIGH-LET,IN-VIVO,CHROMOSOME-ABERRATIONS,KU70/80 KNOCKDOWN,CHROMATIN DOMAINS},
  language     = {eng},
  number       = {2},
  pages        = {149--158},
  title        = {Induction and disappearance of γH2AX foci and formation of micronuclei after exposure of human lymphocytes to 60Co γ-rays and p(66)+Be(40) neutrons},
  url          = {http://dx.doi.org/10.3109/09553002.2014.860252},
  volume       = {90},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: