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Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well characterized human genital organ culture

Lennert Steukers (UGent) , Steven Weyers (UGent) , Xiaoyun Yang (UGent) , Annelies Vandekerckhove (UGent) , Sarah Glorieux (UGent) , Maria Cornelissen (UGent) , Wim Van Den Broeck (UGent) , Marleen Temmerman (UGent) and Hans Nauwynck (UGent)
(2014) JOURNAL OF INFECTIOUS DISEASES. 210(2). p.209-213
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Abstract
We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were found to be expressed in all tissues during the entire menstrual cycle. HVEM expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaquewise mucosal spread across the basement membrane and induced prominent epithelial syncytia.
Keywords
HSV-2, HSV-1, genital herpes, dissemination, mucosa, endocervix, ectocervix, nectin, HVEM, organ culture, ENTRY, FUSION, TYPE-1, WOMEN

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MLA
Steukers, Lennert, Steven Weyers, Xiaoyun Yang, et al. “Mimicking Herpes Simplex Virus 1 and Herpes Simplex Virus 2 Mucosal Behavior in a Well Characterized Human Genital Organ Culture.” JOURNAL OF INFECTIOUS DISEASES 210.2 (2014): 209–213. Print.
APA
Steukers, L., Weyers, S., Yang, X., Vandekerckhove, A., Glorieux, S., Cornelissen, M., Van Den Broeck, W., et al. (2014). Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well characterized human genital organ culture. JOURNAL OF INFECTIOUS DISEASES, 210(2), 209–213.
Chicago author-date
Steukers, Lennert, Steven Weyers, Xiaoyun Yang, Annelies Vandekerckhove, Sarah Glorieux, Maria Cornelissen, Wim Van Den Broeck, Marleen Temmerman, and Hans Nauwynck. 2014. “Mimicking Herpes Simplex Virus 1 and Herpes Simplex Virus 2 Mucosal Behavior in a Well Characterized Human Genital Organ Culture.” Journal of Infectious Diseases 210 (2): 209–213.
Chicago author-date (all authors)
Steukers, Lennert, Steven Weyers, Xiaoyun Yang, Annelies Vandekerckhove, Sarah Glorieux, Maria Cornelissen, Wim Van Den Broeck, Marleen Temmerman, and Hans Nauwynck. 2014. “Mimicking Herpes Simplex Virus 1 and Herpes Simplex Virus 2 Mucosal Behavior in a Well Characterized Human Genital Organ Culture.” Journal of Infectious Diseases 210 (2): 209–213.
Vancouver
1.
Steukers L, Weyers S, Yang X, Vandekerckhove A, Glorieux S, Cornelissen M, et al. Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well characterized human genital organ culture. JOURNAL OF INFECTIOUS DISEASES. 2014;210(2):209–13.
IEEE
[1]
L. Steukers et al., “Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well characterized human genital organ culture,” JOURNAL OF INFECTIOUS DISEASES, vol. 210, no. 2, pp. 209–213, 2014.
@article{4258726,
  abstract     = {We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were found to be expressed in all tissues during the entire menstrual cycle. HVEM expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaquewise mucosal spread across the basement membrane and induced prominent epithelial syncytia.},
  author       = {Steukers, Lennert and Weyers, Steven and Yang, Xiaoyun and Vandekerckhove, Annelies and Glorieux, Sarah and Cornelissen, Maria and Van Den Broeck, Wim and Temmerman, Marleen and Nauwynck, Hans},
  issn         = {0022-1899},
  journal      = {JOURNAL OF INFECTIOUS DISEASES},
  keywords     = {HSV-2,HSV-1,genital herpes,dissemination,mucosa,endocervix,ectocervix,nectin,HVEM,organ culture,ENTRY,FUSION,TYPE-1,WOMEN},
  language     = {eng},
  number       = {2},
  pages        = {209--213},
  title        = {Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well characterized human genital organ culture},
  url          = {http://dx.doi.org/10.1093/infdis/jiu036},
  volume       = {210},
  year         = {2014},
}

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