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Recombinant Clostridium perfringens α toxin as a potential vaccine against bovine enterotoxemia

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Abstract
Clostridium perfringens type A has been shown to be the causative agent of a wide variety of enteric diseases in humans and animals, including bovine enterotoxemia. Enterotoxemia is a sudden death syndrome with necro-hemorrhagic lesions in the small intestine, which mainly affects suckling calves and veal calves [Muylaert et al., 2010 Vet. Rec. 167:64–65]. Predominantly veal calves of beef cattle breeds are affected, and losses due to enterotoxemia may be responsible for up to 20% of total mortality [Lebrun et al., 2010 Vet. Rec. 167:13–22, Pardon et al., 2012 BMC Vet. Res. 8:26]. Recently, α toxin has been proposed as an essential factor for induction of enterotoxemia in veal calves, which introduces the possibility to use it as a vaccine [Verherstraeten et al., 2013 Vet. Res. 44:45]. The use of only the main toxin instead of the whole arsenal of extracellular toxins and enzymes eliminates irrelevant or even immunosuppressive components and therefore may induce a stronger, protective immune response. In this study, we have compared a commercial multivalent vaccine with native α toxin, formalin-inactivated α toxin, and recombinant C-terminal domain of α toxin as vaccine candidates. The native and recombinant α toxin were capable of stimulating higher levels of immune responses compared with the formalin inactivated α toxin and the multivalent vaccine. In addition, the sera from calves immunized with the native and recombinant α toxin also showed higher inhibitory activity against the α toxin in a bioassay. These results suggest that the recombinant α toxin is a potential vaccine candidate against bovine enterotoxemia.
Keywords
bovine enterotoxemia, recombinant α toxin, vaccination

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Chicago
Goossens, Evy, Stefanie Verherstraeten, Bonnie Valgaeren, Bart Pardon, Leen Timbermont, Freddy Haesebrouck, Richard Titball, Richard Ducatelle, Piet Deprez, and Filip Van Immerseel. 2013. “Recombinant Clostridium Perfringens α Toxin as a Potential Vaccine Against Bovine Enterotoxemia.” In Symposium on Gut Health in Production of Food Animals : Program and Abstracts, 14–14. Champaign, IL, USA: Federation of Animal Science Societies (FASS).
APA
Goossens, Evy, Verherstraeten, S., Valgaeren, B., Pardon, B., Timbermont, L., Haesebrouck, F., Titball, R., et al. (2013). Recombinant Clostridium perfringens α toxin as a potential vaccine against bovine enterotoxemia. Symposium on gut health in production of food animals : program and abstracts (pp. 14–14). Presented at the Symposium on Gut Health in Production of Food Animals, Champaign, IL, USA: Federation of Animal Science Societies (FASS).
Vancouver
1.
Goossens E, Verherstraeten S, Valgaeren B, Pardon B, Timbermont L, Haesebrouck F, et al. Recombinant Clostridium perfringens α toxin as a potential vaccine against bovine enterotoxemia. Symposium on gut health in production of food animals : program and abstracts. Champaign, IL, USA: Federation of Animal Science Societies (FASS); 2013. p. 14–14.
MLA
Goossens, Evy, Stefanie Verherstraeten, Bonnie Valgaeren, et al. “Recombinant Clostridium Perfringens α Toxin as a Potential Vaccine Against Bovine Enterotoxemia.” Symposium on Gut Health in Production of Food Animals : Program and Abstracts. Champaign, IL, USA: Federation of Animal Science Societies (FASS), 2013. 14–14. Print.
@inproceedings{4258669,
  abstract     = {Clostridium perfringens type A has been shown to be the causative agent of a wide variety of enteric diseases in humans and animals, including bovine enterotoxemia. Enterotoxemia is a sudden death syndrome with necro-hemorrhagic lesions in the small intestine, which mainly affects suckling calves and veal calves [Muylaert et al., 2010 Vet. Rec. 167:64--65]. Predominantly veal calves of beef cattle breeds are affected, and losses due to enterotoxemia may be responsible for up to 20\% of total mortality [Lebrun et al., 2010 Vet. Rec. 167:13--22, Pardon et al., 2012 BMC Vet. Res. 8:26]. Recently, \ensuremath{\alpha} toxin has been proposed as an essential factor for induction of enterotoxemia in veal calves, which introduces the possibility to use it as a vaccine [Verherstraeten et al., 2013 Vet. Res. 44:45]. The use of only the main toxin instead of the whole arsenal of extracellular toxins and enzymes eliminates irrelevant or even immunosuppressive components and therefore may induce a stronger, protective immune response. In this study, we have compared a commercial multivalent vaccine with native \ensuremath{\alpha} toxin, formalin-inactivated \ensuremath{\alpha} toxin, and recombinant C-terminal domain of \ensuremath{\alpha} toxin as vaccine candidates. The native and recombinant \ensuremath{\alpha} toxin were capable of stimulating higher levels of immune responses compared with the formalin inactivated \ensuremath{\alpha} toxin and the multivalent vaccine. In addition, the sera from calves immunized with the native and recombinant \ensuremath{\alpha} toxin also showed higher inhibitory activity against the \ensuremath{\alpha} toxin in a bioassay.
These results suggest that the recombinant \ensuremath{\alpha} toxin is a potential vaccine candidate against bovine enterotoxemia.},
  articleno    = {abstract 106},
  author       = {Goossens, Evy and Verherstraeten, Stefanie and Valgaeren, Bonnie and Pardon, Bart and Timbermont, Leen and Haesebrouck, Freddy and Titball, Richard and Ducatelle, Richard and Deprez, Piet and Van Immerseel, Filip},
  booktitle    = {Symposium on gut health in production of food animals : program and abstracts},
  language     = {eng},
  location     = {Kansas City, MO, USA},
  pages        = {abstract 106:14--abstract 106:14},
  publisher    = {Federation of Animal Science Societies (FASS)},
  title        = {Recombinant Clostridium perfringens \ensuremath{\alpha} toxin as a potential vaccine against bovine enterotoxemia},
  url          = {http://www.guthealthsymposium.com/2013/2013\_GutHealth\_Program\_WEB.pdf},
  year         = {2013},
}