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A gas chromatographic method for analysis of oxygenated polar fatty acids (epoxy, hydroxy and oxo) is described. The challenge of polar fatty analysis in the past has been interferences due to co-elution of the various compounds present in the complex mixture. The method uses a three step SPE separation technique to fractionate the fatty acids so that each fraction is free of interfering compounds thus reducing co-elution. The epoxy and oxo fatty acids have been analysed in one portion. Due to the high polarity of the hydroxy fatty acids it is only possible to analyse them after silylation. The elution profile of the hydroxyl fatty acids can be simplified by hydrogenation which reduces the number of compounds allowing better chromatographic interpretation. The method has proven to be sensitive and exhibits low LODs which is promising towards more robust analytical methods. Application of the validated method on fresh oil samples has revealed that it is an effective tool to quantitatively screen polar fatty acids in trace amounts and can be very useful for exposure assessment studies.

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MLA
Mubiru, Edward, Kshitij Shrestha, and Bruno De Meulenaer. “Gas Chromatographic Analytical Method for the Analysis of Oxygenated Polar Fatty Acids.” 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of Abstracts. 2014. Print.
APA
Mubiru, E., Shrestha, K., & De Meulenaer, B. (2014). Gas chromatographic analytical method for the analysis of oxygenated polar fatty acids. 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts. Presented at the 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) ; 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3).
Chicago author-date
Mubiru, Edward, Kshitij Shrestha, and Bruno De Meulenaer. 2014. “Gas Chromatographic Analytical Method for the Analysis of Oxygenated Polar Fatty Acids.” In 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of Abstracts.
Chicago author-date (all authors)
Mubiru, Edward, Kshitij Shrestha, and Bruno De Meulenaer. 2014. “Gas Chromatographic Analytical Method for the Analysis of Oxygenated Polar Fatty Acids.” In 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of Abstracts.
Vancouver
1.
Mubiru E, Shrestha K, De Meulenaer B. Gas chromatographic analytical method for the analysis of oxygenated polar fatty acids. 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts. 2014.
IEEE
[1]
E. Mubiru, K. Shrestha, and B. De Meulenaer, “Gas chromatographic analytical method for the analysis of oxygenated polar fatty acids,” in 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts, Brugge, Belgium, 2014.
@inproceedings{4256574,
  abstract     = {A gas chromatographic method for analysis of oxygenated polar fatty acids (epoxy, hydroxy and oxo) is described. The challenge of polar fatty analysis in the past has been interferences due to co-elution of the various compounds present in the complex mixture. The method uses a three step SPE separation technique to fractionate the fatty acids so that each fraction is free of interfering compounds thus reducing co-elution. The epoxy and oxo fatty acids have been analysed in one portion.  Due to the high polarity of the hydroxy fatty acids it is only possible to analyse them after silylation. The elution profile of the hydroxyl fatty acids can be simplified by hydrogenation which reduces the number of compounds allowing better chromatographic interpretation. The method has proven to be sensitive and exhibits low LODs which is promising towards more robust analytical methods. Application of the validated method on fresh oil samples has revealed that it is an effective tool to quantitatively screen polar fatty  acids in trace amounts and can be very useful for exposure assessment studies.},
  author       = {Mubiru, Edward and Shrestha, Kshitij and De Meulenaer, Bruno},
  booktitle    = {13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts},
  language     = {eng},
  location     = {Brugge, Belgium},
  title        = {Gas chromatographic analytical method for the analysis of oxygenated polar fatty acids},
  year         = {2014},
}