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Correlation of dual colour single particle trajectories for improved detection and analysis of interactions in living cells

Hendrik Deschout UGent, Thomas Martens, Dries Vercauteren, Katrien Remaut UGent, Jo Demeester UGent, Stefaan De Smedt UGent, Kristiaan Neyts UGent and Kevin Braeckmans UGent (2013) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 14(8). p.16485-16514
abstract
Interactions between objects inside living cells are often investigated by looking for colocalization between fluorescence microscopy images that are recorded in separate colours corresponding to the fluorescent label of each object. The fundamental limitation of this approach in the case of dynamic objects is that coincidental colocalization cannot be distinguished from true interaction. Instead, correlation between motion trajectories obtained by dual colour single particle tracking provides a much stronger indication of interaction. However, frequently occurring phenomena in living cells, such as immobile phases or transient interactions, can limit the correlation to small parts of the trajectories. The method presented here, developed for the detection of interaction, is based on the correlation inside a window that is scanned along the trajectories, covering different subsets of the positions. This scanning window method was validated by simulations and, as an experimental proof of concept, it was applied to the investigation of the intracellular trafficking of polymeric gene complexes by endosomes in living retinal pigment epithelium cells, which is of interest to ocular gene therapy.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
colocalization, interaction, correlation, endosomal escape, single particle tracking, fluorescence microscopy, diffusion, COLOCALIZATION ANALYSIS, IMAGE CORRELATION, FLUORESCENCE MICROSCOPY, MOLECULE LOCALIZATION, MEMBRANE-PROTEINS, TRACKING, PROBES, DYNAMICS, DELIVERY, COMPLEX
journal title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Int. J. Mol. Sci.
volume
14
issue
8
pages
16485 - 16514
Web of Science type
Article
Web of Science id
000328501300067
JCR category
CHEMISTRY, MULTIDISCIPLINARY
JCR impact factor
2.339 (2013)
JCR rank
52/148 (2013)
JCR quartile
2 (2013)
ISSN
1422-0067
DOI
10.3390/ijms140816485
project
Center for nano- and biophotonics (NB-Photonics)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
4253346
handle
http://hdl.handle.net/1854/LU-4253346
date created
2014-01-28 16:12:15
date last changed
2017-05-12 08:21:16
@article{4253346,
  abstract     = {Interactions between objects inside living cells are often investigated by looking for colocalization between fluorescence microscopy images that are recorded in separate colours corresponding to the fluorescent label of each object. The fundamental limitation of this approach in the case of dynamic objects is that coincidental colocalization cannot be distinguished from true interaction. Instead, correlation between motion trajectories obtained by dual colour single particle tracking provides a much stronger indication of interaction. However, frequently occurring phenomena in living cells, such as immobile phases or transient interactions, can limit the correlation to small parts of the trajectories. The method presented here, developed for the detection of interaction, is based on the correlation inside a window that is scanned along the trajectories, covering different subsets of the positions. This scanning window method was validated by simulations and, as an experimental proof of concept, it was applied to the investigation of the intracellular trafficking of polymeric gene complexes by endosomes in living retinal pigment epithelium cells, which is of interest to ocular gene therapy.},
  author       = {Deschout, Hendrik and Martens, Thomas and Vercauteren, Dries and Remaut, Katrien and Demeester, Jo and De Smedt, Stefaan and Neyts, Kristiaan and Braeckmans, Kevin},
  issn         = {1422-0067},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
  keyword      = {colocalization,interaction,correlation,endosomal escape,single particle tracking,fluorescence microscopy,diffusion,COLOCALIZATION ANALYSIS,IMAGE CORRELATION,FLUORESCENCE MICROSCOPY,MOLECULE LOCALIZATION,MEMBRANE-PROTEINS,TRACKING,PROBES,DYNAMICS,DELIVERY,COMPLEX},
  language     = {eng},
  number       = {8},
  pages        = {16485--16514},
  title        = {Correlation of dual colour single particle trajectories for improved detection and analysis of interactions in living cells},
  url          = {http://dx.doi.org/10.3390/ijms140816485},
  volume       = {14},
  year         = {2013},
}

Chicago
Deschout, Hendrik, Thomas Martens, Dries Vercauteren, Katrien Remaut, Jo Demeester, Stefaan De Smedt, Kristiaan Neyts, and Kevin Braeckmans. 2013. “Correlation of Dual Colour Single Particle Trajectories for Improved Detection and Analysis of Interactions in Living Cells.” International Journal of Molecular Sciences 14 (8): 16485–16514.
APA
Deschout, H., Martens, T., Vercauteren, D., Remaut, K., Demeester, J., De Smedt, S., Neyts, K., et al. (2013). Correlation of dual colour single particle trajectories for improved detection and analysis of interactions in living cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 14(8), 16485–16514.
Vancouver
1.
Deschout H, Martens T, Vercauteren D, Remaut K, Demeester J, De Smedt S, et al. Correlation of dual colour single particle trajectories for improved detection and analysis of interactions in living cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2013;14(8):16485–514.
MLA
Deschout, Hendrik, Thomas Martens, Dries Vercauteren, et al. “Correlation of Dual Colour Single Particle Trajectories for Improved Detection and Analysis of Interactions in Living Cells.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 14.8 (2013): 16485–16514. Print.