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Mice overexpressing β-1,4-galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis

Valerie Vanhooren (UGent) , Roosmarijn Vandenbroucke (UGent) , Sylviane Dewaele (UGent) , Evelien Van Hamme (UGent) , Jody Haigh (UGent) , Tino Hochepied (UGent) and Claude Libert (UGent)
(2013) PLOS ONE. 8(12).
Author
Organization
Abstract
Glycosylation is an essential post-translational modification, which determines the function of proteins and important processes such as inflammation. beta-1,4-galactosyltransferase I (beta GalT1) is a key enzyme involved in the addition of galactose moieties to glycoproteins. Intestinal mucins are glycoproteins that protect the gut barrier against invading pathogens and determine the composition of the intestinal microbiota. Proper glycosylation of mucus is important in this regard. By using ubiquitously expressing beta GalT1 transgenic mice, we found that this enzyme led to strong galactosylation of mucus proteins, isolated from the gut of mice. This galactosylation was associated with a drastic change in composition of gut microbiota, as TG mice had a significantly higher Firmicutes to Bacteroidetes ratio. TG mice were strongly protected against TNF-induced systemic inflammation and lethality. Moreover, beta GalT1 transgenic mice were protected in a model of DSS-induced colitis, at the level of clinical score, loss of body weight, colon length and gut permeability. These studies put beta GalT1 forward as an essential protective player in exacerbated intestinal inflammation. Optimal galactosylation of N-glycans of mucus proteins, determining the bacterial composition of the gut, is a likely mechanism of this function.
Keywords
DNA-SEQUENCING EQUIPMENT, ULCERATIVE-COLITIS, PANETH CELLS, ALPHA-DEFENSINS, BOWEL-DISEASE, N-GLYCANS, GLYCOSYLATION, MUCIN, MICROBIOTA, MUCUS

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MLA
Vanhooren, Valerie, Roosmarijn Vandenbroucke, Sylviane Dewaele, et al. “Mice Overexpressing Β-1,4-galactosyltransferase I Are Resistant to TNF-induced Inflammation and DSS-induced Colitis.” PLOS ONE 8.12 (2013): n. pag. Print.
APA
Vanhooren, V., Vandenbroucke, R., Dewaele, S., Van Hamme, E., Haigh, J., Hochepied, T., & Libert, C. (2013). Mice overexpressing β-1,4-galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis. PLOS ONE, 8(12).
Chicago author-date
Vanhooren, Valerie, Roosmarijn Vandenbroucke, Sylviane Dewaele, Evelien Van Hamme, Jody Haigh, Tino Hochepied, and Claude Libert. 2013. “Mice Overexpressing Β-1,4-galactosyltransferase I Are Resistant to TNF-induced Inflammation and DSS-induced Colitis.” Plos One 8 (12).
Chicago author-date (all authors)
Vanhooren, Valerie, Roosmarijn Vandenbroucke, Sylviane Dewaele, Evelien Van Hamme, Jody Haigh, Tino Hochepied, and Claude Libert. 2013. “Mice Overexpressing Β-1,4-galactosyltransferase I Are Resistant to TNF-induced Inflammation and DSS-induced Colitis.” Plos One 8 (12).
Vancouver
1.
Vanhooren V, Vandenbroucke R, Dewaele S, Van Hamme E, Haigh J, Hochepied T, et al. Mice overexpressing β-1,4-galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis. PLOS ONE. 2013;8(12).
IEEE
[1]
V. Vanhooren et al., “Mice overexpressing β-1,4-galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis,” PLOS ONE, vol. 8, no. 12, 2013.
@article{4252312,
  abstract     = {Glycosylation is an essential post-translational modification, which determines the function of proteins and important processes such as inflammation. beta-1,4-galactosyltransferase I (beta GalT1) is a key enzyme involved in the addition of galactose moieties to glycoproteins. Intestinal mucins are glycoproteins that protect the gut barrier against invading pathogens and determine the composition of the intestinal microbiota. Proper glycosylation of mucus is important in this regard. By using ubiquitously expressing beta GalT1 transgenic mice, we found that this enzyme led to strong galactosylation of mucus proteins, isolated from the gut of mice. This galactosylation was associated with a drastic change in composition of gut microbiota, as TG mice had a significantly higher Firmicutes to Bacteroidetes ratio. TG mice were strongly protected against TNF-induced systemic inflammation and lethality. Moreover, beta GalT1 transgenic mice were protected in a model of DSS-induced colitis, at the level of clinical score, loss of body weight, colon length and gut permeability. These studies put beta GalT1 forward as an essential protective player in exacerbated intestinal inflammation. Optimal galactosylation of N-glycans of mucus proteins, determining the bacterial composition of the gut, is a likely mechanism of this function.},
  articleno    = {e79883},
  author       = {Vanhooren, Valerie and Vandenbroucke, Roosmarijn and Dewaele, Sylviane and Van Hamme, Evelien and Haigh, Jody and Hochepied, Tino and Libert, Claude},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {DNA-SEQUENCING EQUIPMENT,ULCERATIVE-COLITIS,PANETH CELLS,ALPHA-DEFENSINS,BOWEL-DISEASE,N-GLYCANS,GLYCOSYLATION,MUCIN,MICROBIOTA,MUCUS},
  language     = {eng},
  number       = {12},
  pages        = {14},
  title        = {Mice overexpressing β-1,4-galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis},
  url          = {http://dx.doi.org/10.1371/journal.pone.0079883},
  volume       = {8},
  year         = {2013},
}

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