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A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering

(2012) PLOS ONE. 7(12).
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Abstract
Temporo-mandibular joint disc disorders are highly prevalent in adult populations. Autologous chondrocyte implantation is a well-established method for the treatment of several chondral defects. However, very few studies have been carried out using human fibrous chondrocytes from the temporo-mandibular joint (TMJ). One of the main drawbacks associated to chondrocyte cell culture is the possibility that chondrocyte cells kept in culture tend to de-differentiate and to lose cell viability under in in-vitro conditions. In this work, we have isolated human temporo-mandibular joint fibrochondrocytes (TMJF) from human disc and we have used a highly-sensitive technique to determine cell viability, cell proliferation and gene expression of nine consecutive cell passages to determine the most appropriate cell passage for use in tissue engineering and future clinical use. Our results revealed that the most potentially viable and functional cell passages were P5-P6, in which an adequate equilibrium between cell viability and the capability to synthesize all major extracellular matrix components exists. The combined action of pro-apoptotic (TRAF5, PHLDA1) and anti-apoptotic genes (SON, HTT, FAIM2) may explain the differential cell viability levels that we found in this study. These results suggest that TMJF should be used at P5-P6 for cell therapy protocols.
Keywords
TEMPOROMANDIBULAR-JOINT DISC, X-RAY-MICROANALYSIS, ELEMENTAL CONCENTRATIONS, ARTICULAR-CARTILAGE, ENDOTHELIAL-CELLS, STEM-CELLS, CHONDROCYTES, DIFFERENTIATION, DISORDERS, THERAPY

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Citation

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MLA
Garzón, Ingrid, Víctor Sebastián Carriel Araya, Ana Belén Marín-Fernández, et al. “A Combined Approach for the Assessment of Cell Viability and Cell Functionality of Human Fibrochondrocytes for Use in Tissue Engineering.” PLOS ONE 7.12 (2012): n. pag. Print.
APA
Garzón, Ingrid, Carriel Araya, V. S., Marín-Fernández, A. B., Oliveira, A. C., Garrido-Gómez, J., Campos, A., Sánchez-Quevedo, M. del C., et al. (2012). A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering. PLOS ONE, 7(12).
Chicago author-date
Garzón, Ingrid, Víctor Sebastián Carriel Araya, Ana Belén Marín-Fernández, Ana Celeste Oliveira, Juan Garrido-Gómez, Antonio Campos, Maria del Carmen Sánchez-Quevedo, and Miguel Alaminos. 2012. “A Combined Approach for the Assessment of Cell Viability and Cell Functionality of Human Fibrochondrocytes for Use in Tissue Engineering.” Plos One 7 (12).
Chicago author-date (all authors)
Garzón, Ingrid, Víctor Sebastián Carriel Araya, Ana Belén Marín-Fernández, Ana Celeste Oliveira, Juan Garrido-Gómez, Antonio Campos, Maria del Carmen Sánchez-Quevedo, and Miguel Alaminos. 2012. “A Combined Approach for the Assessment of Cell Viability and Cell Functionality of Human Fibrochondrocytes for Use in Tissue Engineering.” Plos One 7 (12).
Vancouver
1.
Garzón I, Carriel Araya VS, Marín-Fernández AB, Oliveira AC, Garrido-Gómez J, Campos A, et al. A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering. PLOS ONE. 2012;7(12).
IEEE
[1]
I. Garzón et al., “A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering,” PLOS ONE, vol. 7, no. 12, 2012.
@article{4251279,
  abstract     = {Temporo-mandibular joint disc disorders are highly prevalent in adult populations. Autologous chondrocyte implantation is a well-established method for the treatment of several chondral defects. However, very few studies have been carried out using human fibrous chondrocytes from the temporo-mandibular joint (TMJ). One of the main drawbacks associated to chondrocyte cell culture is the possibility that chondrocyte cells kept in culture tend to de-differentiate and to lose cell viability under in in-vitro conditions. In this work, we have isolated human temporo-mandibular joint fibrochondrocytes (TMJF) from human disc and we have used a highly-sensitive technique to determine cell viability, cell proliferation and gene expression of nine consecutive cell passages to determine the most appropriate cell passage for use in tissue engineering and future clinical use. Our results revealed that the most potentially viable and functional cell passages were P5-P6, in which an adequate equilibrium between cell viability and the capability to synthesize all major extracellular matrix components exists. The combined action of pro-apoptotic (TRAF5, PHLDA1) and anti-apoptotic genes (SON, HTT, FAIM2) may explain the differential cell viability levels that we found in this study. These results suggest that TMJF should be used at P5-P6 for cell therapy protocols.},
  articleno    = {e51961},
  author       = {Garzón, Ingrid and Carriel Araya, Víctor Sebastián and Marín-Fernández, Ana Belén and Oliveira, Ana Celeste and Garrido-Gómez, Juan and Campos, Antonio and Sánchez-Quevedo, Maria del Carmen and Alaminos, Miguel},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {TEMPOROMANDIBULAR-JOINT DISC,X-RAY-MICROANALYSIS,ELEMENTAL CONCENTRATIONS,ARTICULAR-CARTILAGE,ENDOTHELIAL-CELLS,STEM-CELLS,CHONDROCYTES,DIFFERENTIATION,DISORDERS,THERAPY},
  language     = {eng},
  number       = {12},
  title        = {A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering},
  url          = {http://dx.doi.org/10.1371/journal.pone.0051961},
  volume       = {7},
  year         = {2012},
}

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