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Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer

Tijl Vermassen (UGent) , Charles Van Praet (UGent) , Dieter Vanderschaeghe (UGent) , Thomas Maenhout (UGent) , Nicolaas Lumen (UGent) , Nico Callewaert (UGent) , Piet Hoebeke (UGent) , Simon Van Belle (UGent) , Sylvie Rottey (UGent) and Joris Delanghe (UGent)
(2014) ELECTROPHORESIS. 35(7). p.1017-1024
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Abstract
Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems. Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker. We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures. N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026). Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10g/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p 0.001). In conclusion, N-glycosylation profiling demonstrated differences between BPH and PCa. These changes could lead to the discovery of a new biomarker for PCa.
Keywords
Asparagine-linked glycosylation, Diagnostic marker, Benign prostate hyperplasia, MEN, MARKER, UPDATE, DISEASE, BIOMARKERS, Prostate cancer, Prostate-specific antigen, SERUM N-GLYCOME, ALTERED GLYCOSYLATION, ANTIGEN, PSA, HYPERPLASIA

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Chicago
Vermassen, Tijl, Charles Van Praet, Dieter Vanderschaeghe, Thomas Maenhout, Nicolaas Lumen, Nico Callewaert, Piet Hoebeke, Simon Van Belle, Sylvie Rottey, and Joris Delanghe. 2014. “Capillary Electrophoresis of Urinary Prostate Glycoproteins Assists in the Diagnosis of Prostate Cancer.” Electrophoresis 35 (7): 1017–1024.
APA
Vermassen, T., Van Praet, C., Vanderschaeghe, D., Maenhout, T., Lumen, N., Callewaert, N., Hoebeke, P., et al. (2014). Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer. ELECTROPHORESIS, 35(7), 1017–1024.
Vancouver
1.
Vermassen T, Van Praet C, Vanderschaeghe D, Maenhout T, Lumen N, Callewaert N, et al. Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer. ELECTROPHORESIS. 2014;35(7):1017–24.
MLA
Vermassen, Tijl, Charles Van Praet, Dieter Vanderschaeghe, et al. “Capillary Electrophoresis of Urinary Prostate Glycoproteins Assists in the Diagnosis of Prostate Cancer.” ELECTROPHORESIS 35.7 (2014): 1017–1024. Print.
@article{4250897,
  abstract     = {Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems. Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker. We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures. N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026). Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10g/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p 0.001). In conclusion, N-glycosylation profiling demonstrated differences between BPH and PCa. These changes could lead to the discovery of a new biomarker for PCa.},
  author       = {Vermassen, Tijl and Van Praet, Charles and Vanderschaeghe, Dieter and Maenhout, Thomas and Lumen, Nicolaas and Callewaert, Nico and Hoebeke, Piet and Van Belle, Simon and Rottey, Sylvie and Delanghe, Joris},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {Asparagine-linked glycosylation,Diagnostic marker,Benign prostate hyperplasia,MEN,MARKER,UPDATE,DISEASE,BIOMARKERS,Prostate cancer,Prostate-specific antigen,SERUM N-GLYCOME,ALTERED GLYCOSYLATION,ANTIGEN,PSA,HYPERPLASIA},
  language     = {eng},
  number       = {7},
  pages        = {1017--1024},
  title        = {Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer},
  url          = {http://dx.doi.org/10.1002/elps.201300332},
  volume       = {35},
  year         = {2014},
}

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