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A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis

Kathleen Machiels, Marie Joossens UGent, João Sabino, Vicky De Preter, Ingrid Arijs, Venessa Eeckhaut UGent, Vera Ballet, Karoline Claes, Filip Van Immerseel UGent, Kristin Verbeke, et al. (2014) GUT. 63(8). p.1275-1283
abstract
OBJECTIVE: Bacteria play an important role in the onset and perpetuation of intestinal inflammation in inflammatory bowel disease (IBD). Unlike in Crohn's disease (CD), in which dysbiosis has been better characterised, in ulcerative colitis (UC), only small cohorts have been studied and showed conflicting data. Therefore, we evaluated in a large cohort if the microbial signature described in CD is also present in UC, and if we could characterise predominant dysbiosis in UC. To assess the functional impact of dysbiosis, we quantified the bacterial metabolites. DESIGN: The predominant microbiota from 127 UC patients and 87 age and sex-matched controls was analysed using denaturing gradient gel electrophoresis (DGGE) analysis. Differences were quantitatively validated using real-time PCR. Metabolites were quantified using gas chromatography-mass spectrometry. RESULTS: Based on DGGE analysis, the microbial signature previously described in CD was not present in UC. Real-time PCR analysis revealed a lower abundance of Roseburia hominis (p<0.0001) and Faecalibacterium prausnitzii (p<0.0001) in UC patients compared to controls. Both species showed an inverse correlation with disease activity. Short-chain fatty acids (SCFA) were reduced in UC patients (p=0.014), but no direct correlation between SCFA and the identified bacteria was found. CONCLUSIONS: The composition of the fecal microbiota of UC patients differs from that of healthy individuals: we found a reduction in R hominis and F prausnitzii, both well-known butyrate-producing bacteria of the Firmicutes phylum. These results underscore the importance of dysbiosis in IBD but suggest that different bacterial species contribute to the pathogenesis of UC and CD
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DIVERSITY, Butyrate, EXPRESSION, COLONIC-MUCOSA, BUTYRATE ENEMAS, 5-AMINOSALICYLIC ACID, CROHNS-DISEASE, INHIBITION, KAPPA-B ACTIVATION, DISTAL ULCERATIVE-COLITIS, Inflammation, Epithelial Barrier, GUT MICROBIOTA, IBD, Probiotics
journal title
GUT
Gut
volume
63
issue
8
pages
1275 - 1283
Web of Science type
Article
Web of Science id
000339164200013
JCR category
GASTROENTEROLOGY & HEPATOLOGY
JCR impact factor
14.66 (2014)
JCR rank
2/76 (2014)
JCR quartile
1 (2014)
ISSN
0017-5749
DOI
10.1136/gutjnl-2013-304833
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4250224
handle
http://hdl.handle.net/1854/LU-4250224
date created
2014-01-27 11:57:03
date last changed
2018-01-22 15:02:47
@article{4250224,
  abstract     = {OBJECTIVE: Bacteria play an important role in the onset and perpetuation of intestinal inflammation in inflammatory bowel disease (IBD). Unlike in Crohn's disease (CD), in which dysbiosis has been better characterised, in ulcerative colitis (UC), only small cohorts have been studied and showed conflicting data. Therefore, we evaluated in a large cohort if the microbial signature described in CD is also present in UC, and if we could characterise predominant dysbiosis in UC. To assess the functional impact of dysbiosis, we quantified the bacterial metabolites.
DESIGN: The predominant microbiota from 127 UC patients and 87 age and sex-matched controls was analysed using denaturing gradient gel electrophoresis (DGGE) analysis. Differences were quantitatively validated using real-time PCR. Metabolites were quantified using gas chromatography-mass spectrometry.
RESULTS: Based on DGGE analysis, the microbial signature previously described in CD was not present in UC. Real-time PCR analysis revealed a lower abundance of Roseburia hominis (p{\textlangle}0.0001) and Faecalibacterium prausnitzii (p{\textlangle}0.0001) in UC patients compared to controls. Both species showed an inverse correlation with disease activity. Short-chain fatty acids (SCFA) were reduced in UC patients (p=0.014), but no direct correlation between SCFA and the identified bacteria was found.
CONCLUSIONS: The composition of the fecal microbiota of UC patients differs from that of healthy individuals: we found a reduction in R hominis and F prausnitzii, both well-known butyrate-producing bacteria of the Firmicutes phylum. These results underscore the importance of dysbiosis in IBD but suggest that different bacterial species contribute to the pathogenesis of UC and CD},
  author       = {Machiels, Kathleen and Joossens, Marie and Sabino, Jo{\~a}o and De Preter, Vicky and Arijs, Ingrid and Eeckhaut, Venessa and Ballet, Vera and Claes, Karoline and Van Immerseel, Filip and Verbeke, Kristin and Ferrante, Marc and Verhaegen, Jan and Rutgeerts, Paul and Vermeire, S{\'e}verine},
  issn         = {0017-5749},
  journal      = {GUT},
  keyword      = {DIVERSITY,Butyrate,EXPRESSION,COLONIC-MUCOSA,BUTYRATE ENEMAS,5-AMINOSALICYLIC ACID,CROHNS-DISEASE,INHIBITION,KAPPA-B ACTIVATION,DISTAL ULCERATIVE-COLITIS,Inflammation,Epithelial Barrier,GUT MICROBIOTA,IBD,Probiotics},
  language     = {eng},
  number       = {8},
  pages        = {1275--1283},
  title        = {A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis},
  url          = {http://dx.doi.org/10.1136/gutjnl-2013-304833},
  volume       = {63},
  year         = {2014},
}

Chicago
Machiels, Kathleen, Marie Joossens, João Sabino, Vicky De Preter, Ingrid Arijs, Venessa Eeckhaut, Vera Ballet, et al. 2014. “A Decrease of the Butyrate-producing Species Roseburia Hominis and Faecalibacterium Prausnitzii Defines Dysbiosis in Patients with Ulcerative Colitis.” GUT 63 (8): 1275–1283.
APA
Machiels, Kathleen, Joossens, M., Sabino, J., De Preter, V., Arijs, I., Eeckhaut, V., Ballet, V., et al. (2014). A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis. GUT, 63(8), 1275–1283.
Vancouver
1.
Machiels K, Joossens M, Sabino J, De Preter V, Arijs I, Eeckhaut V, et al. A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis. GUT. 2014;63(8):1275–83.
MLA
Machiels, Kathleen, Marie Joossens, João Sabino, et al. “A Decrease of the Butyrate-producing Species Roseburia Hominis and Faecalibacterium Prausnitzii Defines Dysbiosis in Patients with Ulcerative Colitis.” GUT 63.8 (2014): 1275–1283. Print.