Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties
- Author
- William A Braff, Dana Willner, Philip Hugenholtz, Korneel Rabaey (UGent) and Cullen R Buie
- Organization
- Project
- Abstract
- Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.
- Keywords
- INSULATOR-BASED DIELECTROPHORESIS, MUCOID PSEUDOMONAS-AERUGINOSA, STREPTOCOCCUS-MITIS, CYSTIC-FIBROSIS, ANTIBIOTIC-RESISTANCE, BIOFILM FORMATION, ADHESION, BINDING, PROTEINS, DEVICES
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-4245999
- MLA
- Braff, William A., et al. “Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties.” PLOS ONE, vol. 8, no. 10, 2013, doi:10.1371/journal.pone.0076751.
- APA
- Braff, W. A., Willner, D., Hugenholtz, P., Rabaey, K., & Buie, C. R. (2013). Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties. PLOS ONE, 8(10). https://doi.org/10.1371/journal.pone.0076751
- Chicago author-date
- Braff, William A, Dana Willner, Philip Hugenholtz, Korneel Rabaey, and Cullen R Buie. 2013. “Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties.” PLOS ONE 8 (10). https://doi.org/10.1371/journal.pone.0076751.
- Chicago author-date (all authors)
- Braff, William A, Dana Willner, Philip Hugenholtz, Korneel Rabaey, and Cullen R Buie. 2013. “Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties.” PLOS ONE 8 (10). doi:10.1371/journal.pone.0076751.
- Vancouver
- 1.Braff WA, Willner D, Hugenholtz P, Rabaey K, Buie CR. Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties. PLOS ONE. 2013;8(10).
- IEEE
- [1]W. A. Braff, D. Willner, P. Hugenholtz, K. Rabaey, and C. R. Buie, “Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties,” PLOS ONE, vol. 8, no. 10, 2013.
@article{4245999, abstract = {{Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.}}, articleno = {{e76751}}, author = {{Braff, William A and Willner, Dana and Hugenholtz, Philip and Rabaey, Korneel and Buie, Cullen R}}, issn = {{1932-6203}}, journal = {{PLOS ONE}}, keywords = {{INSULATOR-BASED DIELECTROPHORESIS,MUCOID PSEUDOMONAS-AERUGINOSA,STREPTOCOCCUS-MITIS,CYSTIC-FIBROSIS,ANTIBIOTIC-RESISTANCE,BIOFILM FORMATION,ADHESION,BINDING,PROTEINS,DEVICES}}, language = {{eng}}, number = {{10}}, pages = {{7}}, title = {{Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties}}, url = {{http://doi.org/10.1371/journal.pone.0076751}}, volume = {{8}}, year = {{2013}}, }
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