Advanced search
1 file | 1.26 MB Add to list

Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties

(2013) PLOS ONE. 8(10).
Author
Organization
Project
Abstract
Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.
Keywords
INSULATOR-BASED DIELECTROPHORESIS, MUCOID PSEUDOMONAS-AERUGINOSA, STREPTOCOCCUS-MITIS, CYSTIC-FIBROSIS, ANTIBIOTIC-RESISTANCE, BIOFILM FORMATION, ADHESION, BINDING, PROTEINS, DEVICES

Downloads

  • fetchObject.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.26 MB

Citation

Please use this url to cite or link to this publication:

MLA
Braff, William A., et al. “Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties.” PLOS ONE, vol. 8, no. 10, 2013, doi:10.1371/journal.pone.0076751.
APA
Braff, W. A., Willner, D., Hugenholtz, P., Rabaey, K., & Buie, C. R. (2013). Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties. PLOS ONE, 8(10). https://doi.org/10.1371/journal.pone.0076751
Chicago author-date
Braff, William A, Dana Willner, Philip Hugenholtz, Korneel Rabaey, and Cullen R Buie. 2013. “Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties.” PLOS ONE 8 (10). https://doi.org/10.1371/journal.pone.0076751.
Chicago author-date (all authors)
Braff, William A, Dana Willner, Philip Hugenholtz, Korneel Rabaey, and Cullen R Buie. 2013. “Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties.” PLOS ONE 8 (10). doi:10.1371/journal.pone.0076751.
Vancouver
1.
Braff WA, Willner D, Hugenholtz P, Rabaey K, Buie CR. Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties. PLOS ONE. 2013;8(10).
IEEE
[1]
W. A. Braff, D. Willner, P. Hugenholtz, K. Rabaey, and C. R. Buie, “Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties,” PLOS ONE, vol. 8, no. 10, 2013.
@article{4245999,
  abstract     = {{Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.}},
  articleno    = {{e76751}},
  author       = {{Braff, William A and Willner, Dana and Hugenholtz, Philip and Rabaey, Korneel and Buie, Cullen R}},
  issn         = {{1932-6203}},
  journal      = {{PLOS ONE}},
  keywords     = {{INSULATOR-BASED DIELECTROPHORESIS,MUCOID PSEUDOMONAS-AERUGINOSA,STREPTOCOCCUS-MITIS,CYSTIC-FIBROSIS,ANTIBIOTIC-RESISTANCE,BIOFILM FORMATION,ADHESION,BINDING,PROTEINS,DEVICES}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{7}},
  title        = {{Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties}},
  url          = {{http://doi.org/10.1371/journal.pone.0076751}},
  volume       = {{8}},
  year         = {{2013}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: