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Burkholderia dilworthii sp. nov. isolated from Lebeckia ambigua root nodules

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Abstract
Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556(T) being most closely related to Burkholderia caledonica LMG 23644(T) (98.70% 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937(T) (98.50%). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain W5M3556(T) (=LMG 27173(T)=HAMBI 3353(T)).
Keywords
SEQUENCE DATA, LEGUMES, NITROGEN, ELEOCHARIS-DULCIS, EMENDED DESCRIPTION, DEOXYRIBONUCLEIC-ACID, HIGHLY ACIDIC SWAMPS, MIMOSA SPP., STRAINS, BACTERIA

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MLA
De Meyer, Sofie, Margo Cnockaert, Julie K Ardley, et al. “Burkholderia Dilworthii Sp. Nov. Isolated from Lebeckia Ambigua Root Nodules.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.4 (2014): 1090–1095. Print.
APA
De Meyer, Sofie, Cnockaert, M., Ardley, J. K., Van Wyk, B.-E., Vandamme, P., & Howieson, J. G. (2014). Burkholderia dilworthii sp. nov. isolated from Lebeckia ambigua root nodules. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(4), 1090–1095.
Chicago author-date
De Meyer, Sofie, Margo Cnockaert, Julie K Ardley, Ben-Erik Van Wyk, Peter Vandamme, and John G Howieson. 2014. “Burkholderia Dilworthii Sp. Nov. Isolated from Lebeckia Ambigua Root Nodules.” International Journal of Systematic and Evolutionary Microbiology 64 (4): 1090–1095.
Chicago author-date (all authors)
De Meyer, Sofie, Margo Cnockaert, Julie K Ardley, Ben-Erik Van Wyk, Peter Vandamme, and John G Howieson. 2014. “Burkholderia Dilworthii Sp. Nov. Isolated from Lebeckia Ambigua Root Nodules.” International Journal of Systematic and Evolutionary Microbiology 64 (4): 1090–1095.
Vancouver
1.
De Meyer S, Cnockaert M, Ardley JK, Van Wyk B-E, Vandamme P, Howieson JG. Burkholderia dilworthii sp. nov. isolated from Lebeckia ambigua root nodules. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(4):1090–5.
IEEE
[1]
S. De Meyer, M. Cnockaert, J. K. Ardley, B.-E. Van Wyk, P. Vandamme, and J. G. Howieson, “Burkholderia dilworthii sp. nov. isolated from Lebeckia ambigua root nodules,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 64, no. 4, pp. 1090–1095, 2014.
@article{4238749,
  abstract     = {Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556(T) being most closely related to Burkholderia caledonica LMG 23644(T) (98.70% 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937(T) (98.50%). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain W5M3556(T) (=LMG 27173(T)=HAMBI 3353(T)).},
  author       = {De Meyer, Sofie and Cnockaert, Margo and Ardley, Julie K and Van Wyk, Ben-Erik and Vandamme, Peter and Howieson, John G},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {SEQUENCE DATA,LEGUMES,NITROGEN,ELEOCHARIS-DULCIS,EMENDED DESCRIPTION,DEOXYRIBONUCLEIC-ACID,HIGHLY ACIDIC SWAMPS,MIMOSA SPP.,STRAINS,BACTERIA},
  language     = {eng},
  number       = {4},
  pages        = {1090--1095},
  title        = {Burkholderia dilworthii sp. nov. isolated from Lebeckia ambigua root nodules},
  url          = {http://dx.doi.org/10.1099/ijs.0.058602-0},
  volume       = {64},
  year         = {2014},
}

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