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Application of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to detect the abuse of 17β-estradiol in cattle

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Abstract
Although the ability to differentiate between endogenous steroids and synthetic homologues on the basis of their C-13/C-12 isotopic ratio has been known for over a decade, this technique has been scarcely implemented for food safety purposes. In this study, a method was developed using gas chromatography mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to demonstrate the abuse of 17 beta-estradiol in cattle, by comparison of the 13C/12C ratios of the main metabolite 17 alpha-estradiol and an endogenous reference compound (ERC), 5-androstene-3 beta,17 alpha-diol, in bovine urine. The intermediate precisions were determined as 0.46 and 0.26 parts per thousand for 5-androstene-3 beta,17 alpha-diol and 17 alpha-estrachol, respectively. This is, to the authors' knowledge, the first reported use of GC-MS/C/IRMS for the analysis of steroid compounds for food safety issues.
Keywords
natural hormones, estradiol, steroids, GC-MS/C/IRMS, urine, bovine, food safety, abuse, CARBON-ISOTOPE ANALYSIS, ANABOLIC PRACTICES, BOVINE URINE, TESTOSTERONE, POPULATION, STEROIDS, SCREEN, MISUSE

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Citation

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MLA
Janssens, Geert, Sven Mangelinckx, Dirk Courtheyn, et al. “Application of Gas Chromatography-mass Spectrometry/combustion/isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) to Detect the Abuse of 17β-estradiol in Cattle.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 61.30 (2013): 7242–7249. Print.
APA
Janssens, Geert, Mangelinckx, S., Courtheyn, D., Prévost, S., De Poorter, G., De Kimpe, N., & Le Bizec, B. (2013). Application of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to detect the abuse of 17β-estradiol in cattle. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 61(30), 7242–7249.
Chicago author-date
Janssens, Geert, Sven Mangelinckx, Dirk Courtheyn, Stéphanie Prévost, Geert De Poorter, Norbert De Kimpe, and Bruno Le Bizec. 2013. “Application of Gas Chromatography-mass Spectrometry/combustion/isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) to Detect the Abuse of 17β-estradiol in Cattle.” Journal of Agricultural and Food Chemistry 61 (30): 7242–7249.
Chicago author-date (all authors)
Janssens, Geert, Sven Mangelinckx, Dirk Courtheyn, Stéphanie Prévost, Geert De Poorter, Norbert De Kimpe, and Bruno Le Bizec. 2013. “Application of Gas Chromatography-mass Spectrometry/combustion/isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) to Detect the Abuse of 17β-estradiol in Cattle.” Journal of Agricultural and Food Chemistry 61 (30): 7242–7249.
Vancouver
1.
Janssens G, Mangelinckx S, Courtheyn D, Prévost S, De Poorter G, De Kimpe N, et al. Application of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to detect the abuse of 17β-estradiol in cattle. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2013;61(30):7242–9.
IEEE
[1]
G. Janssens et al., “Application of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to detect the abuse of 17β-estradiol in cattle,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 61, no. 30, pp. 7242–7249, 2013.
@article{4237946,
  abstract     = {Although the ability to differentiate between endogenous steroids and synthetic homologues on the basis of their C-13/C-12 isotopic ratio has been known for over a decade, this technique has been scarcely implemented for food safety purposes. In this study, a method was developed using gas chromatography mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to demonstrate the abuse of 17 beta-estradiol in cattle, by comparison of the 13C/12C ratios of the main metabolite 17 alpha-estradiol and an endogenous reference compound (ERC), 5-androstene-3 beta,17 alpha-diol, in bovine urine. The intermediate precisions were determined as 0.46 and 0.26 parts per thousand for 5-androstene-3 beta,17 alpha-diol and 17 alpha-estrachol, respectively. This is, to the authors' knowledge, the first reported use of GC-MS/C/IRMS for the analysis of steroid compounds for food safety issues.},
  author       = {Janssens, Geert and Mangelinckx, Sven and Courtheyn, Dirk and Prévost, Stéphanie and De Poorter, Geert and De Kimpe, Norbert and Le Bizec, Bruno},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keywords     = {natural hormones,estradiol,steroids,GC-MS/C/IRMS,urine,bovine,food safety,abuse,CARBON-ISOTOPE ANALYSIS,ANABOLIC PRACTICES,BOVINE URINE,TESTOSTERONE,POPULATION,STEROIDS,SCREEN,MISUSE},
  language     = {eng},
  number       = {30},
  pages        = {7242--7249},
  title        = {Application of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to detect the abuse of 17β-estradiol in cattle},
  url          = {http://dx.doi.org/10.1021/jf401797p1},
  volume       = {61},
  year         = {2013},
}

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