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Rice folate enhancement through metabolic engineering has an impact on rice seed metabolism, but does not affect the expression of the endogenous folate biosythesis genes

Dieter Blancquaert, Jeroen Van Daele, Serguei Storojenko UGent, Christophe Stove UGent, Willy Lambert and Dominique Van Der Straeten UGent (2013) PLANT MOLECULAR BIOLOGY. 83(4-5). p.329-349
abstract
Folates are key-players in one-carbon metabolism in all organisms. However, only micro-organisms and plants are able to synthesize folates de novo and humans rely entirely on their diet as a sole folate source. As a consequence, folate deficiency is a global problem. Although different strategies are currently implemented to fight folate deficiency, up until now, all of them have their own drawbacks. As an alternative and complementary means to those classical strategies, folate biofortification of rice by metabolic engineering was successfully achieved a couple of years ago. To gain more insight into folate biosynthesis regulation and the effect of folate enhancement on general rice seed metabolism, a transcriptomic study was conducted in developing transgenic rice seeds, overexpressing 2 genes of the folate biosynthetic pathway. Upon folate enhancement, the expression of 235 genes was significantly altered. Here, we show that rice folate biofortification has an important effect on folate dependent, seed developmental and plant stress response/defense processes, but does not affect the expression of the endogenous folate biosynthesis genes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CYCLOHYDROLASE I, MOLYBDENUM COFACTOR, ABSCISIC-ACID, ARABIDOPSIS, PLANTS, HOMOCYSTEINE, PROTEIN, Folic acid, Metabolic engineering, Biofortification, Transcriptomic profiling, Microarray, P-AMINOBENZOATE BRANCH, FOLIC-ACID, S-ADENOSYLMETHIONINE, Folate
journal title
PLANT MOLECULAR BIOLOGY
Plant Mol.Biol.
volume
83
issue
4-5
pages
329 - 349
Web of Science type
Article
Web of Science id
000325116600004
JCR category
PLANT SCIENCES
JCR impact factor
4.072 (2013)
JCR rank
20/199 (2013)
JCR quartile
1 (2013)
ISSN
0167-4412
DOI
10.1007/s11103-013-0091-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4237019
handle
http://hdl.handle.net/1854/LU-4237019
date created
2014-01-20 10:27:07
date last changed
2016-12-19 15:45:02
@article{4237019,
  abstract     = {Folates are key-players in one-carbon metabolism in all organisms. However, only micro-organisms and plants are able to synthesize folates de novo and humans rely entirely on their diet as a sole folate source. As a consequence, folate deficiency is a global problem. Although different strategies are currently implemented to fight folate deficiency, up until now, all of them have their own drawbacks. As an alternative and complementary means to those classical strategies, folate biofortification of rice by metabolic engineering was successfully achieved a couple of years ago. To gain more insight into folate biosynthesis regulation and the effect of folate enhancement on general rice seed metabolism, a transcriptomic study was conducted in developing transgenic rice seeds, overexpressing 2 genes of the folate biosynthetic pathway. Upon folate enhancement, the expression of 235 genes was significantly altered. Here, we show that rice folate biofortification has an important effect on folate dependent, seed developmental and plant stress response/defense processes, but does not affect the expression of the endogenous folate biosynthesis genes.},
  author       = {Blancquaert, Dieter and Van Daele, Jeroen and Storojenko, Serguei and Stove, Christophe and Lambert, Willy and Van Der Straeten, Dominique},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  keyword      = {CYCLOHYDROLASE I,MOLYBDENUM COFACTOR,ABSCISIC-ACID,ARABIDOPSIS,PLANTS,HOMOCYSTEINE,PROTEIN,Folic acid,Metabolic engineering,Biofortification,Transcriptomic profiling,Microarray,P-AMINOBENZOATE BRANCH,FOLIC-ACID,S-ADENOSYLMETHIONINE,Folate},
  language     = {eng},
  number       = {4-5},
  pages        = {329--349},
  title        = {Rice folate enhancement through metabolic engineering has an impact on rice seed metabolism, but does not affect the expression of the endogenous folate biosythesis genes},
  url          = {http://dx.doi.org/10.1007/s11103-013-0091-7},
  volume       = {83},
  year         = {2013},
}

Chicago
Blancquaert, Dieter, Jeroen Van Daele, Serguei Storojenko, Christophe Stove, Willy Lambert, and Dominique Van Der Straeten. 2013. “Rice Folate Enhancement Through Metabolic Engineering Has an Impact on Rice Seed Metabolism, but Does Not Affect the Expression of the Endogenous Folate Biosythesis Genes.” Plant Molecular Biology 83 (4-5): 329–349.
APA
Blancquaert, D., Van Daele, J., Storojenko, S., Stove, C., Lambert, W., & Van Der Straeten, D. (2013). Rice folate enhancement through metabolic engineering has an impact on rice seed metabolism, but does not affect the expression of the endogenous folate biosythesis genes. PLANT MOLECULAR BIOLOGY, 83(4-5), 329–349.
Vancouver
1.
Blancquaert D, Van Daele J, Storojenko S, Stove C, Lambert W, Van Der Straeten D. Rice folate enhancement through metabolic engineering has an impact on rice seed metabolism, but does not affect the expression of the endogenous folate biosythesis genes. PLANT MOLECULAR BIOLOGY. 2013;83(4-5):329–49.
MLA
Blancquaert, Dieter, Jeroen Van Daele, Serguei Storojenko, et al. “Rice Folate Enhancement Through Metabolic Engineering Has an Impact on Rice Seed Metabolism, but Does Not Affect the Expression of the Endogenous Folate Biosythesis Genes.” PLANT MOLECULAR BIOLOGY 83.4-5 (2013): 329–349. Print.