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Blood-brain barrier transport of short proline-rich antimicrobial peptides

(2014) PROTEIN AND PEPTIDE LETTERS. 21(4). p.399-406
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Abstract
Infections by antibiotic-resistant bacteria are becoming a great risk for human health, leading to an urgent need for new efficient antibacterial therapies. The short, proline-rich antimicrobial peptides from insects gained a lot of interest as a potential antibacterial treatment, having a low toxicity profile and being mainly active against Gram-negative bacteria. To know whether these antimicrobial peptides can be used for the treatment of cerebral infections, the blood-brain barrier transport characteristics of these peptides were investigated. This study describes the results of the in vivo blood-brain barrier experiments in mice, as well as the in vitro metabolic stability in mouse plasma and brain of apidaecin Api137, oncocin, drosocin and drosocin Pro5Hyp. The four investigated peptides showed a significant influx into the brain with a Kin ranging between 0.37 and 0.86 μL/g × min and brain distribution volumes of 19.6 to 25.8 μL/g. Only for drosocin, a significant efflux was determined, with a kout of 0.22 min-1. After entering the brain, oncocin was for approximately 80% trapped in the endothelial cells, while the other peptides reached the brain parenchyma for about 70%. All peptides were stable in plasma and brain during the experiments, with estimated metabolic half-lives ranging between 47 min and 637 min. We conclude that the investigated short, proline-rich antimicrobial peptides show an influx into the brain, which make them a promising antibacterial treatment of cerebral infections.
Keywords
Antimicrobial peptides, blood-brain barrier, cell-penetrating peptides, bio-distribution, stability, in vivo, transport, CELL-PENETRATING PEPTIDES, VITRO METABOLIC STABILITY, GRAM-NEGATIVE PATHOGENS, INFECTIONS, ANALOGS, RESISTANCE, OBESTATIN, PROTEINS

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Chicago
Stalmans, Sofie, Evelien Wynendaele, Nathalie Bracke, Daniel Knappe, Ralf Hoffmann, Kathelijne Peremans, Ingeborgh Polis, Christian Burvenich, and Bart De Spiegeleer. 2014. “Blood-brain Barrier Transport of Short Proline-rich Antimicrobial Peptides.” Protein and Peptide Letters 21 (4): 399–406.
APA
Stalmans, S., Wynendaele, E., Bracke, N., Knappe, D., Hoffmann, R., Peremans, K., Polis, I., et al. (2014). Blood-brain barrier transport of short proline-rich antimicrobial peptides. PROTEIN AND PEPTIDE LETTERS, 21(4), 399–406.
Vancouver
1.
Stalmans S, Wynendaele E, Bracke N, Knappe D, Hoffmann R, Peremans K, et al. Blood-brain barrier transport of short proline-rich antimicrobial peptides. PROTEIN AND PEPTIDE LETTERS. 2014;21(4):399–406.
MLA
Stalmans, Sofie, Evelien Wynendaele, Nathalie Bracke, et al. “Blood-brain Barrier Transport of Short Proline-rich Antimicrobial Peptides.” PROTEIN AND PEPTIDE LETTERS 21.4 (2014): 399–406. Print.
@article{4236178,
  abstract     = {Infections by antibiotic-resistant bacteria are becoming a great risk for human health, leading to an urgent need for new efficient antibacterial therapies. The short, proline-rich antimicrobial peptides from insects gained a lot of interest as a potential antibacterial treatment, having a low toxicity profile and being mainly active against Gram-negative bacteria. To know whether these antimicrobial peptides can be used for the treatment of cerebral infections, the blood-brain barrier transport characteristics of these peptides were investigated. This study describes the results of the in vivo blood-brain barrier experiments in mice, as well as the in vitro metabolic stability in mouse plasma and brain of apidaecin Api137, oncocin, drosocin and drosocin Pro5Hyp. The four investigated peptides showed a significant influx into the brain with a Kin ranging between 0.37 and 0.86 \ensuremath{\mu}L/g {\texttimes} min and brain distribution volumes of 19.6 to 25.8 \ensuremath{\mu}L/g. Only for drosocin, a significant efflux was determined, with a kout of 0.22 min-1. After entering the brain, oncocin was for approximately 80\% trapped in the endothelial cells, while the other peptides reached the brain parenchyma for about 70\%. All peptides were stable in plasma and brain during the experiments, with estimated metabolic half-lives ranging between 47 min and 637 min. We conclude that the investigated short, proline-rich antimicrobial peptides show an influx into the brain, which make them a promising antibacterial treatment of cerebral infections.},
  author       = {Stalmans, Sofie and Wynendaele, Evelien and Bracke, Nathalie and Knappe, Daniel and Hoffmann, Ralf and Peremans, Kathelijne and Polis, Ingeborgh and Burvenich, Christian and De Spiegeleer, Bart},
  issn         = {0929-8665},
  journal      = {PROTEIN AND PEPTIDE LETTERS},
  language     = {eng},
  number       = {4},
  pages        = {399--406},
  title        = {Blood-brain barrier transport of short proline-rich antimicrobial peptides},
  url          = {http://dx.doi.org/10.2174/09298665113206660110},
  volume       = {21},
  year         = {2014},
}

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