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Collaborative investigation of matrix effects in mycotoxin determination by high performance liquid chromatography coupled to mass spectrometry

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Abstract
Liquid chromatography coupled to mass spectrometry (LC-MS) is a commonly used technique for mycotoxin determination in food and feed. However, accuracy and reliability of the obtained results can suffer from ion suppression/enhancement caused by co-eluting matrix components. Inter-laboratory study concerning relative and absolute matrix effects (MEs) in various food and feed matrices was carried out. The applicability of commonly used strategies in ME reduction were tested in the quantitative determination of nivalenol, deoxynivalenol, fumonisin and B-2, and zearalenone in complex feed matrices. 'In house' validated LC-MS methods were applied. The relative MEs were assessed on the different food/feed sample sets. Cereal based- and hay/silage feed were used as complex model matrices for absolute ME assessment. The relative MEs within the different sample sets were in range of 3-72%. The absolute MEs for all analytes were in the range of 60-100% and 7-187% for cereal based and hay silage feed, respectively. The use of any purification technique helped to improve absolute MEs for some analytes. Changes in LC conditions did not eliminate ME occurrence. Shifting from electrospray ionization in positive mode (ESI+) to electrospray ionization in negative mode (ESI-) improved MEs for early eluting analytes in hay/silage feed. The choice of ion source highly depended on the analyte-matrix combination.
Keywords
Fusarium toxins, complex matrix, LC-MS, matrix effects, sample preparation, PRESSURE CHEMICAL-IONIZATION, LC-MS/MS METHOD, CEREALS, TRICHOTHECENES, EXTRACTION, SUPPRESSION, VALIDATION, MAIZE, DRUGS, FEED

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MLA
Malachova, Alexandra, Michael Sulyok, Rainer Schuhmacher, et al. “Collaborative Investigation of Matrix Effects in Mycotoxin Determination by High Performance Liquid Chromatography Coupled to Mass Spectrometry.” QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS 5.2 (2013): 91–103. Print.
APA
Malachova, A., Sulyok, M., Schuhmacher, R., Berthiller, F., Hajslova, J., Veprikova, Z., Zachariasova, M., et al. (2013). Collaborative investigation of matrix effects in mycotoxin determination by high performance liquid chromatography coupled to mass spectrometry. QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS, 5(2), 91–103.
Chicago author-date
Malachova, Alexandra, Michael Sulyok, Rainer Schuhmacher, Franz Berthiller, Jana Hajslova, Zdenka Veprikova, Milena Zachariasova, et al. 2013. “Collaborative Investigation of Matrix Effects in Mycotoxin Determination by High Performance Liquid Chromatography Coupled to Mass Spectrometry.” Quality Assurance and Safety of Crops & Foods 5 (2): 91–103.
Chicago author-date (all authors)
Malachova, Alexandra, Michael Sulyok, Rainer Schuhmacher, Franz Berthiller, Jana Hajslova, Zdenka Veprikova, Milena Zachariasova, Veronica Maria Teresa Lattanzio, Sarah De Saeger, José Diana Di Mavungu, Svetlana Malysheva, Scarlett Biselli, Ole Winkelmann, Andreas Breidbach, Simon Hird, and Rudolf Krska. 2013. “Collaborative Investigation of Matrix Effects in Mycotoxin Determination by High Performance Liquid Chromatography Coupled to Mass Spectrometry.” Quality Assurance and Safety of Crops & Foods 5 (2): 91–103.
Vancouver
1.
Malachova A, Sulyok M, Schuhmacher R, Berthiller F, Hajslova J, Veprikova Z, et al. Collaborative investigation of matrix effects in mycotoxin determination by high performance liquid chromatography coupled to mass spectrometry. QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS. 2013;5(2):91–103.
IEEE
[1]
A. Malachova et al., “Collaborative investigation of matrix effects in mycotoxin determination by high performance liquid chromatography coupled to mass spectrometry,” QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS, vol. 5, no. 2, pp. 91–103, 2013.
@article{4231264,
  abstract     = {Liquid chromatography coupled to mass spectrometry (LC-MS) is a commonly used technique for mycotoxin determination in food and feed. However, accuracy and reliability of the obtained results can suffer from ion suppression/enhancement caused by co-eluting matrix components. Inter-laboratory study concerning relative and absolute matrix effects (MEs) in various food and feed matrices was carried out. The applicability of commonly used strategies in ME reduction were tested in the quantitative determination of nivalenol, deoxynivalenol, fumonisin and B-2, and zearalenone in complex feed matrices. 'In house' validated LC-MS methods were applied. The relative MEs were assessed on the different food/feed sample sets. Cereal based- and hay/silage feed were used as complex model matrices for absolute ME assessment. The relative MEs within the different sample sets were in range of 3-72%. The absolute MEs for all analytes were in the range of 60-100% and 7-187% for cereal based and hay silage feed, respectively. The use of any purification technique helped to improve absolute MEs for some analytes. Changes in LC conditions did not eliminate ME occurrence. Shifting from electrospray ionization in positive mode (ESI+) to electrospray ionization in negative mode (ESI-) improved MEs for early eluting analytes in hay/silage feed. The choice of ion source highly depended on the analyte-matrix combination.},
  author       = {Malachova, Alexandra and Sulyok, Michael and Schuhmacher, Rainer and Berthiller, Franz and Hajslova, Jana and Veprikova, Zdenka and Zachariasova, Milena and Lattanzio, Veronica Maria Teresa and De Saeger, Sarah and Diana Di Mavungu, José and Malysheva, Svetlana and Biselli, Scarlett and Winkelmann, Ole and Breidbach, Andreas and Hird, Simon and Krska, Rudolf},
  issn         = {1757-8361},
  journal      = {QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS},
  keywords     = {Fusarium toxins,complex matrix,LC-MS,matrix effects,sample preparation,PRESSURE CHEMICAL-IONIZATION,LC-MS/MS METHOD,CEREALS,TRICHOTHECENES,EXTRACTION,SUPPRESSION,VALIDATION,MAIZE,DRUGS,FEED},
  language     = {eng},
  number       = {2},
  pages        = {91--103},
  title        = {Collaborative investigation of matrix effects in mycotoxin determination by high performance liquid chromatography coupled to mass spectrometry},
  url          = {http://dx.doi.org/10.3920/QAS2012.0213},
  volume       = {5},
  year         = {2013},
}

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