Advanced search
1 file | 537.55 KB

Sampling of wheat dust and subsequent analysis of deoxynivalenol by LC-MS/MS

Melanie Sanders (UGent) , Marthe De Boevre (UGent) , Frédéric Dumoulin (UGent) , Christel Detavernier (UGent) , Freya Martens (UGent) , Christof Van Poucke (UGent) , Mia Eeckhout (UGent) and Sarah De Saeger (UGent)
Author
Organization
Abstract
An LC-MS/MS method was developed and validated for the determination of deoxynivalenol in wheat dust. Extraction was carried out with acetonitrile/water/acetic acid (79/20/1, v/v/v) followed by a hexane defatting step. Analysis was performed using a Waters Acquity UPLC system coupled to a Quattro Premier XE mass spectrometer. The method was validated according to the criteria mentioned in Commission Decision 2002/657/EC. Due to a high contamination level of wheat dust compared to wheat, limit of detection and limit of quantitation levels of 358 ng/g and 717 ng/g, respectively, were obtained. A small survey was executed on raw wheat materials and their corresponding dust samples (n = 12). The samples were analyzed according to the developed procedure. A linear correlation (R-2 = 0.941) was found for the deoxynivalenol concentration in dust versus the deoxynivalenol concentration in wheat Therefore, it would be possible to estimate the cereal contamination through dust contamination.
Keywords
wheat dust, validation, LC-MS/MS, deoxynivalenol, correlation, OF-THE-ART, TRICHOTHECENE MYCOTOXINS, MASS-SPECTROMETRY, GRAIN DUST, FOOD, PRODUCTS, CEREALS, FEED

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 537.55 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Sanders, Melanie, Marthe De Boevre, Frédéric Dumoulin, Christel Detavernier, Freya Martens, Christof Van Poucke, Mia Eeckhout, and Sarah De Saeger. 2013. “Sampling of Wheat Dust and Subsequent Analysis of Deoxynivalenol by LC-MS/MS.” Journal of Agricultural and Food Chemistry 61 (26): 6259–6264.
APA
Sanders, Melanie, De Boevre, M., Dumoulin, F., Detavernier, C., Martens, F., Van Poucke, C., Eeckhout, M., et al. (2013). Sampling of wheat dust and subsequent analysis of deoxynivalenol by LC-MS/MS. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 61(26), 6259–6264.
Vancouver
1.
Sanders M, De Boevre M, Dumoulin F, Detavernier C, Martens F, Van Poucke C, et al. Sampling of wheat dust and subsequent analysis of deoxynivalenol by LC-MS/MS. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2013;61(26):6259–64.
MLA
Sanders, Melanie, Marthe De Boevre, Frédéric Dumoulin, et al. “Sampling of Wheat Dust and Subsequent Analysis of Deoxynivalenol by LC-MS/MS.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 61.26 (2013): 6259–6264. Print.
@article{4231247,
  abstract     = {An LC-MS/MS method was developed and validated for the determination of deoxynivalenol in wheat dust. Extraction was carried out with acetonitrile/water/acetic acid (79/20/1, v/v/v) followed by a hexane defatting step. Analysis was performed using a Waters Acquity UPLC system coupled to a Quattro Premier XE mass spectrometer. The method was validated according to the criteria mentioned in Commission Decision 2002/657/EC. Due to a high contamination level of wheat dust compared to wheat, limit of detection and limit of quantitation levels of 358 ng/g and 717 ng/g, respectively, were obtained. A small survey was executed on raw wheat materials and their corresponding dust samples (n = 12). The samples were analyzed according to the developed procedure. A linear correlation (R-2 = 0.941) was found for the deoxynivalenol concentration in dust versus the deoxynivalenol concentration in wheat Therefore, it would be possible to estimate the cereal contamination through dust contamination.},
  author       = {Sanders, Melanie and De Boevre, Marthe and Dumoulin, Frédéric and Detavernier, Christel and Martens, Freya and Van Poucke, Christof and Eeckhout, Mia and De Saeger, Sarah},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keywords     = {wheat dust,validation,LC-MS/MS,deoxynivalenol,correlation,OF-THE-ART,TRICHOTHECENE MYCOTOXINS,MASS-SPECTROMETRY,GRAIN DUST,FOOD,PRODUCTS,CEREALS,FEED},
  language     = {eng},
  number       = {26},
  pages        = {6259--6264},
  title        = {Sampling of wheat dust and subsequent analysis of deoxynivalenol by LC-MS/MS},
  url          = {http://dx.doi.org/10.1021/jf401323s},
  volume       = {61},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: