Advanced search
1 file | 1.39 MB

Tightly controlled WRKY23 expression mediates Arabidopsis embryo development

(2013) EMBO REPORTS. 14(12). p.1136-1142
Author
Organization
Abstract
The development of a multicellular embryo from a single zygote is a complex and highly organized process that is far from understood. In higher plants, apical-basal patterning mechanisms are crucial to correctly specify root and shoot stem cell niches that will sustain and drive post-embryonic plant growth and development. The auxin-responsive AtWRKY23 transcription factor is expressed from early embryogenesis onwards and the timing and localization of its expression overlaps with the root stem cell niche. Knocking down WRKY23 transcript levels or expression of a dominant-negative WRKY23 version via a translational fusion with the SRDX repressor domain affected both apical-basal axis formation as well as installation of the root stem cell niche. WRKY23 expression is affected by two well-known root stem cell specification mechanisms, that is, SHORTROOT and MONOPTEROS-BODENLOS signalling and can partially rescue the root-forming inability of mp embryos. On the basis of these results, we postulate that a tightly controlled WRKY23 expression is involved in the regulation of both auxin-dependent and auxin-independent signalling pathways towards stem cell specification.
Keywords
ROOT DEVELOPMENT, GENES, TRANSCRIPTION FACTOR, STEM-CELL NICHE, WRKY, embryogenesis, Arabidopsis, AXIS, REGULATORS, THALIANA, PROTEINS, ENCODES

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.39 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Grunewald, Wim, Ive De Smet, Bert De Rybel, Helena Robert, Brigitte Van De Cotte, Viola Willemsen, Godelieve Gheysen, Dolf Weijers, Jiri Friml, and Tom Beeckman. 2013. “Tightly Controlled WRKY23 Expression Mediates Arabidopsis Embryo Development.” Embo Reports 14 (12): 1136–1142.
APA
Grunewald, W., De Smet, I., De Rybel, B., Robert, H., Van De Cotte, B., Willemsen, V., Gheysen, G., et al. (2013). Tightly controlled WRKY23 expression mediates Arabidopsis embryo development. EMBO REPORTS, 14(12), 1136–1142.
Vancouver
1.
Grunewald W, De Smet I, De Rybel B, Robert H, Van De Cotte B, Willemsen V, et al. Tightly controlled WRKY23 expression mediates Arabidopsis embryo development. EMBO REPORTS. 2013;14(12):1136–42.
MLA
Grunewald, Wim, Ive De Smet, Bert De Rybel, et al. “Tightly Controlled WRKY23 Expression Mediates Arabidopsis Embryo Development.” EMBO REPORTS 14.12 (2013): 1136–1142. Print.
@article{4224234,
  abstract     = {The development of a multicellular embryo from a single zygote is a complex and highly organized process that is far from understood. In higher plants, apical-basal patterning mechanisms are crucial to correctly specify root and shoot stem cell niches that will sustain and drive post-embryonic plant growth and development. The auxin-responsive AtWRKY23 transcription factor is expressed from early embryogenesis onwards and the timing and localization of its expression overlaps with the root stem cell niche. Knocking down WRKY23 transcript levels or expression of a dominant-negative WRKY23 version via a translational fusion with the SRDX repressor domain affected both apical-basal axis formation as well as installation of the root stem cell niche. WRKY23 expression is affected by two well-known root stem cell specification mechanisms, that is, SHORTROOT and MONOPTEROS-BODENLOS signalling and can partially rescue the root-forming inability of mp embryos. On the basis of these results, we postulate that a tightly controlled WRKY23 expression is involved in the regulation of both auxin-dependent and auxin-independent signalling pathways towards stem cell specification.},
  author       = {Grunewald, Wim and De Smet, Ive and De Rybel, Bert and Robert, Helena and Van De Cotte, Brigitte and Willemsen, Viola and Gheysen, Godelieve and Weijers, Dolf and Friml, Jiri and Beeckman, Tom},
  issn         = {1469-221X},
  journal      = {EMBO REPORTS},
  keyword      = {ROOT DEVELOPMENT,GENES,TRANSCRIPTION FACTOR,STEM-CELL NICHE,WRKY,embryogenesis,Arabidopsis,AXIS,REGULATORS,THALIANA,PROTEINS,ENCODES},
  language     = {eng},
  number       = {12},
  pages        = {1136--1142},
  title        = {Tightly controlled WRKY23 expression mediates Arabidopsis embryo development},
  url          = {http://dx.doi.org/10.1038/embor.2013.169},
  volume       = {14},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: