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Constitutively active UVR8 photoreceptor variant in Arabidopsis

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Abstract
Arabidopsis thaliana UV RESISTANCE LOCUS 8 (UVR8) is a UV-B photoreceptor that initiates photomorphogenic responses underlying acclimation and UV-B tolerance in plants. UVR8 is a homodimer in its ground state, and UV-B exposure results in its instantaneous monomerization followed by interaction with CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), a major factor in UV-B signaling. UV-B photoreception by UVR8 is based on intrinsic tryptophan aromatic amino acid residues, with tryptophan-285 as the main chromophore. We generated transgenic plants expressing UVR8 with a single amino acid change of tryptophan-285 to alanine. UVR8(W285A) appears monomeric and shows UV-B-independent interaction with COP1. Phenotypically, the plants expressing UVR8(W285A) exhibit constitutive photomorphogenesis associated with constitutive activation of target genes, elevated levels of anthocyanins, and enhanced, acclimation-independent UV-B tolerance. Moreover, we have identified COP1, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 and 2 (RUP1 and RUP2), and the SUPPRESSOR OF PHYA-105 (SPA) family as proteins copurifying with UVR8(W285A). Whereas COP1, RUP1, and RUP2 are known to directly interact with UVR8, we show that SPA1 interacts with UVR8 indirectly through COP1. We conclude that UVR8(W285A) is a constitutively active UVR8 photoreceptor variant in Arabidopsis, as is consistent with the crucial importance of monomer formation and COP1 binding for UVR8 activity.
Keywords
STRESS ACCLIMATION, TRANSCRIPTION FACTOR HY5, COP1, LIGHT, PROTEIN, INDUCTION, COMPLEXES, PHYTOCHROME, abiotic stress, ultraviolet-B, signal transduction, B-INDUCED PHOTOMORPHOGENESIS, SIGNAL-TRANSDUCTION

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Chicago
Heijde, Marc, Melanie Binkert, Ruohe Yin, Florence Ares-Orpel, Luca Rizzini, Eveline Van De Slijke, Geert Persiau, et al. 2013. “Constitutively Active UVR8 Photoreceptor Variant in Arabidopsis.” Proceedings of the National Academy of Sciences of the United States of America 110 (50): 20326–20331.
APA
Heijde, M., Binkert, M., Yin, R., Ares-Orpel, F., Rizzini, L., Van De Slijke, E., Persiau, G., et al. (2013). Constitutively active UVR8 photoreceptor variant in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(50), 20326–20331.
Vancouver
1.
Heijde M, Binkert M, Yin R, Ares-Orpel F, Rizzini L, Van De Slijke E, et al. Constitutively active UVR8 photoreceptor variant in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2013;110(50):20326–31.
MLA
Heijde, Marc, Melanie Binkert, Ruohe Yin, et al. “Constitutively Active UVR8 Photoreceptor Variant in Arabidopsis.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.50 (2013): 20326–20331. Print.
@article{4220555,
  abstract     = {Arabidopsis thaliana UV RESISTANCE LOCUS 8 (UVR8) is a UV-B photoreceptor that initiates photomorphogenic responses underlying acclimation and UV-B tolerance in plants. UVR8 is a homodimer in its ground state, and UV-B exposure results in its instantaneous monomerization followed by interaction with CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), a major factor in UV-B signaling. UV-B photoreception by UVR8 is based on intrinsic tryptophan aromatic amino acid residues, with tryptophan-285 as the main chromophore. We generated transgenic plants expressing UVR8 with a single amino acid change of tryptophan-285 to alanine. UVR8(W285A) appears monomeric and shows UV-B-independent interaction with COP1. Phenotypically, the plants expressing UVR8(W285A) exhibit constitutive photomorphogenesis associated with constitutive activation of target genes, elevated levels of anthocyanins, and enhanced, acclimation-independent UV-B tolerance. Moreover, we have identified COP1, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 and 2 (RUP1 and RUP2), and the SUPPRESSOR OF PHYA-105 (SPA) family as proteins copurifying with UVR8(W285A). Whereas COP1, RUP1, and RUP2 are known to directly interact with UVR8, we show that SPA1 interacts with UVR8 indirectly through COP1. We conclude that UVR8(W285A) is a constitutively active UVR8 photoreceptor variant in Arabidopsis, as is consistent with the crucial importance of monomer formation and COP1 binding for UVR8 activity.},
  author       = {Heijde, Marc and Binkert, Melanie and Yin, Ruohe and Ares-Orpel, Florence and Rizzini, Luca and Van De Slijke, Eveline and Persiau, Geert and Nolf, Jonah and Gevaert, Kris and De Jaeger, Geert and Ulm, Roman},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {STRESS ACCLIMATION,TRANSCRIPTION FACTOR HY5,COP1,LIGHT,PROTEIN,INDUCTION,COMPLEXES,PHYTOCHROME,abiotic stress,ultraviolet-B,signal transduction,B-INDUCED PHOTOMORPHOGENESIS,SIGNAL-TRANSDUCTION},
  language     = {eng},
  number       = {50},
  pages        = {20326--20331},
  title        = {Constitutively active UVR8 photoreceptor variant in Arabidopsis},
  url          = {http://dx.doi.org/10.1073/pnas.1314336110},
  volume       = {110},
  year         = {2013},
}

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