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Asn₃, a reliable, robust, and universal lock mass for improved accuracy in LC-MS and LC-MS/MS

An Staes (UGent) , Jonathan Vandenbussche (UGent) , Hans Demol (UGent) , Marc Goethals (UGent) , Sule Yilmaz-Rumpf (UGent) , Niels Hulstaert (UGent) , Sven Degroeve (UGent) , Pieter Kelchtermans (UGent) , Lennart Martens (UGent) and Kris Gevaert (UGent)
(2013) ANALYTICAL CHEMISTRY. 85(22). p.11054-11060
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
The use of internal calibrants (the so-called lock mass approach) provides much greater accuracy in mass spectrometry based proteomics. However, the polydimethylcyclosiloxane (PCM) peaks commonly used for this purpose are quite unreliable, leading to missing calibrant peaks in spectra and correspondingly lower mass measurement accuracy. Therefore, we here introduce a universally applicable and robust internal calibrant, the tripeptide Asn(3). We show that Asn(3) is a substantial improvement over PCM both in terms of consistent detection and resulting mass measurement accuracy. Asn(3) is also very easy to adopt in the lab, as it requires only minor adjustments to the analytical setup.
Keywords
DATABASE, PROTEINS, PEPTIDES, INJECTION, PRIDE, IDENTIFICATION, SPECTROMETRY, PROTEOMICS

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Citation

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Chicago
Staes, An, Jonathan Vandenbussche, Hans Demol, Marc Goethals, Sule Yilmaz-Rumpf, Niels Hulstaert, Sven Degroeve, Pieter Kelchtermans, Lennart Martens, and Kris Gevaert. 2013. “Asn₃, a Reliable, Robust, and Universal Lock Mass for Improved Accuracy in LC-MS and LC-MS/MS.” Analytical Chemistry 85 (22): 11054–11060.
APA
Staes, A., Vandenbussche, J., Demol, H., Goethals, M., Yilmaz-Rumpf, S., Hulstaert, N., Degroeve, S., et al. (2013). Asn₃, a reliable, robust, and universal lock mass for improved accuracy in LC-MS and LC-MS/MS. ANALYTICAL CHEMISTRY, 85(22), 11054–11060.
Vancouver
1.
Staes A, Vandenbussche J, Demol H, Goethals M, Yilmaz-Rumpf S, Hulstaert N, et al. Asn₃, a reliable, robust, and universal lock mass for improved accuracy in LC-MS and LC-MS/MS. ANALYTICAL CHEMISTRY. 2013;85(22):11054–60.
MLA
Staes, An, Jonathan Vandenbussche, Hans Demol, et al. “Asn₃, a Reliable, Robust, and Universal Lock Mass for Improved Accuracy in LC-MS and LC-MS/MS.” ANALYTICAL CHEMISTRY 85.22 (2013): 11054–11060. Print.
@article{4220352,
  abstract     = {The use of internal calibrants (the so-called lock mass approach) provides much greater accuracy in mass spectrometry based proteomics. However, the polydimethylcyclosiloxane (PCM) peaks commonly used for this purpose are quite unreliable, leading to missing calibrant peaks in spectra and correspondingly lower mass measurement accuracy. Therefore, we here introduce a universally applicable and robust internal calibrant, the tripeptide Asn(3). We show that Asn(3) is a substantial improvement over PCM both in terms of consistent detection and resulting mass measurement accuracy. Asn(3) is also very easy to adopt in the lab, as it requires only minor adjustments to the analytical setup.},
  author       = {Staes, An and Vandenbussche, Jonathan and Demol, Hans and Goethals, Marc and Yilmaz-Rumpf, Sule and Hulstaert, Niels and Degroeve, Sven and Kelchtermans, Pieter and Martens, Lennart and Gevaert, Kris},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  language     = {eng},
  number       = {22},
  pages        = {11054--11060},
  title        = {Asn\unmatched{2083}, a reliable, robust, and universal lock mass for improved accuracy in LC-MS and LC-MS/MS},
  url          = {http://dx.doi.org/10.1021/ac4027093},
  volume       = {85},
  year         = {2013},
}

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