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Beta-actin is involved in modulating erythropoiesis during development by fine-tuning Gata2 expression levels

Davina Tondeleir (UGent) , Benjamin Drogat (UGent) , Karolina Slowicka (UGent) , Karima Bakkali (UGent) , Sona Bartunkova (UGent) , Steven Goossens (UGent) , Jody Haigh (UGent) and Christophe Ampe (UGent)
(2013) PLOS ONE. 8(6).
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Abstract
The functions of actin family members during development are poorly understood. To investigate the role of beta-actin in mammalian development, a beta-actin knockout mouse model was used. Homozygous beta-actin knockout mice are lethal at embryonic day (E) 10.5. At E10.25 beta-actin knockout embryos are growth retarded and display a pale yolk sac and embryo proper that is suggestive of altered erythropoiesis. Here we report that lack of beta-actin resulted in a block of primitive and definitive hematopoietic development. Reduced levels of Gata2, were associated to this phenotype. Consistently, ChIP analysis revealed multiple binding sites for beta-actin in the Gata2 promoter. Gata2 mRNA levels were almost completely rescued by expression of an erythroid lineage restricted ROSA26-promotor based GATA2 transgene. As a result, erythroid differentiation was restored and the knockout embryos showed significant improvement in yolk sac and embryo vascularization. These results provide new molecular insights for a novel function of beta-actin in erythropoiesis by modulating the expression levels of Gata2 in vivo.
Keywords
SKELETAL, ERYTHROID DEVELOPMENT, TRANSCRIPTION FACTOR GATA-2, MESSENGER-RNA, MICE LACKING, GAMMA-ACTIN, CELL-GROWTH, IN-VITRO, ZINC-FINGER GENE, DIFFERENTIATION

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MLA
Tondeleir, Davina, et al. “Beta-Actin Is Involved in Modulating Erythropoiesis during Development by Fine-Tuning Gata2 Expression Levels.” PLOS ONE, vol. 8, no. 6, 2013, doi:10.1371/journal.pone.0067855.
APA
Tondeleir, D., Drogat, B., Slowicka, K., Bakkali, K., Bartunkova, S., Goossens, S., … Ampe, C. (2013). Beta-actin is involved in modulating erythropoiesis during development by fine-tuning Gata2 expression levels. PLOS ONE, 8(6). https://doi.org/10.1371/journal.pone.0067855
Chicago author-date
Tondeleir, Davina, Benjamin Drogat, Karolina Slowicka, Karima Bakkali, Sona Bartunkova, Steven Goossens, Jody Haigh, and Christophe Ampe. 2013. “Beta-Actin Is Involved in Modulating Erythropoiesis during Development by Fine-Tuning Gata2 Expression Levels.” PLOS ONE 8 (6). https://doi.org/10.1371/journal.pone.0067855.
Chicago author-date (all authors)
Tondeleir, Davina, Benjamin Drogat, Karolina Slowicka, Karima Bakkali, Sona Bartunkova, Steven Goossens, Jody Haigh, and Christophe Ampe. 2013. “Beta-Actin Is Involved in Modulating Erythropoiesis during Development by Fine-Tuning Gata2 Expression Levels.” PLOS ONE 8 (6). doi:10.1371/journal.pone.0067855.
Vancouver
1.
Tondeleir D, Drogat B, Slowicka K, Bakkali K, Bartunkova S, Goossens S, et al. Beta-actin is involved in modulating erythropoiesis during development by fine-tuning Gata2 expression levels. PLOS ONE. 2013;8(6).
IEEE
[1]
D. Tondeleir et al., “Beta-actin is involved in modulating erythropoiesis during development by fine-tuning Gata2 expression levels,” PLOS ONE, vol. 8, no. 6, 2013.
@article{4216028,
  abstract     = {{The functions of actin family members during development are poorly understood. To investigate the role of beta-actin in mammalian development, a beta-actin knockout mouse model was used. Homozygous beta-actin knockout mice are lethal at embryonic day (E) 10.5. At E10.25 beta-actin knockout embryos are growth retarded and display a pale yolk sac and embryo proper that is suggestive of altered erythropoiesis. Here we report that lack of beta-actin resulted in a block of primitive and definitive hematopoietic development. Reduced levels of Gata2, were associated to this phenotype. Consistently, ChIP analysis revealed multiple binding sites for beta-actin in the Gata2 promoter. Gata2 mRNA levels were almost completely rescued by expression of an erythroid lineage restricted ROSA26-promotor based GATA2 transgene. As a result, erythroid differentiation was restored and the knockout embryos showed significant improvement in yolk sac and embryo vascularization. These results provide new molecular insights for a novel function of beta-actin in erythropoiesis by modulating the expression levels of Gata2 in vivo.}},
  articleno    = {{e67855}},
  author       = {{Tondeleir, Davina and Drogat, Benjamin and Slowicka, Karolina and Bakkali, Karima and Bartunkova, Sona and Goossens, Steven and Haigh, Jody and Ampe, Christophe}},
  issn         = {{1932-6203}},
  journal      = {{PLOS ONE}},
  keywords     = {{SKELETAL,ERYTHROID DEVELOPMENT,TRANSCRIPTION FACTOR GATA-2,MESSENGER-RNA,MICE LACKING,GAMMA-ACTIN,CELL-GROWTH,IN-VITRO,ZINC-FINGER GENE,DIFFERENTIATION}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{12}},
  title        = {{Beta-actin is involved in modulating erythropoiesis during development by fine-tuning Gata2 expression levels}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0067855}},
  volume       = {{8}},
  year         = {{2013}},
}

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