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Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis

Tomasz Nodzyński (UGent) , Ioan Mugurel Feraru (UGent) , Sibylle Hirsch (UGent) , Riet De Rycke (UGent) , Claudiu Niculaes (UGent) , Wout Boerjan (UGent) , Jelle Van Leene (UGent) , Geert De Jaeger (UGent) , Steffen Vanneste (UGent) and Jiri Friml (UGent)
(2013) MOLECULAR PLANT. 6(6). p.1849-1862
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Arabidopsis mutants defective in subunits of the retromer complex VPS35A and VPS29 show comparable defects in subcellular trafficking and ectopic intracellular accumulation of membrane proteins. These retromer components mediate together the function of the prevacuolar compartment (PVC) and trafficking to the lytic vacuole.Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 (pat3) mutant that we identified by an epifluorescence-based forward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1GFP. While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compartments (PVC) with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A (VPS35A), a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lytic vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting.
Keywords
DEPENDENT AUXIN GRADIENTS, GREEN FLUORESCENT PROTEIN, SEED STORAGE PROTEINS, TRANS GOLGI NETWORK, VACUOLAR SORTING RECEPTORS, Arabidopsis thaliana, vacuolar trafficking, prevacuolar compartment (PVC), VPS29, VPS35, retromer, MULTIVESICULAR BODIES, SHOOT GRAVITROPISM, CELL POLARITY, MEMBRANE RECRUITMENT, PLASMA MEMBRANE

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Citation

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Chicago
Nodzyński, Tomasz, Ioan Mugurel Feraru, Sibylle Hirsch, Riet De Rycke, Claudiu Niculaes, Wout Boerjan, Jelle Van Leene, Geert De Jaeger, Steffen Vanneste, and Jiri Friml. 2013. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” Molecular Plant 6 (6): 1849–1862.
APA
Nodzyński, T., Feraru, I. M., Hirsch, S., De Rycke, R., Niculaes, C., Boerjan, W., Van Leene, J., et al. (2013). Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. MOLECULAR PLANT, 6(6), 1849–1862.
Vancouver
1.
Nodzyński T, Feraru IM, Hirsch S, De Rycke R, Niculaes C, Boerjan W, et al. Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. MOLECULAR PLANT. 2013;6(6):1849–62.
MLA
Nodzyński, Tomasz, Ioan Mugurel Feraru, Sibylle Hirsch, et al. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” MOLECULAR PLANT 6.6 (2013): 1849–1862. Print.
@article{4215719,
  abstract     = {Arabidopsis mutants defective in subunits of the retromer complex VPS35A and VPS29 show comparable defects in subcellular trafficking and ectopic intracellular accumulation of membrane proteins. These retromer components mediate together the function of the prevacuolar compartment (PVC) and trafficking to the lytic vacuole.Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 (pat3) mutant that we identified by an epifluorescence-based forward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1GFP. While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compartments (PVC) with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A (VPS35A), a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lytic vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting.},
  author       = {Nodzy\'{n}ski, Tomasz and Feraru, Ioan Mugurel and Hirsch, Sibylle and De Rycke, Riet and Niculaes, Claudiu and Boerjan, Wout and Van Leene, Jelle and De Jaeger, Geert and Vanneste, Steffen and Friml, Jiri},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  language     = {eng},
  number       = {6},
  pages        = {1849--1862},
  title        = {Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis},
  url          = {http://dx.doi.org/10.1093/mp/sst044},
  volume       = {6},
  year         = {2013},
}

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