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First isolation of 'Brachyspira hampsonii' from pigs in Europe

Maxime Mahu (UGent) , Ellen de Jong (UGent) , Nele De Pauw (UGent) , Lien Vande Maele (UGent) , Virginie Vandenbroucke (UGent) , Tamara Vandersmissen, Cora Miry (UGent) , Frank Pasmans (UGent) , Freddy Haesebrouck (UGent) , An Martel (UGent) , et al.
(2014) VETERINARY RECORD. 174(2).
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Abstract
Swine dysentery in Europe is classically attributed to Brachyspira hyodysenteriae. However, other Brachyspira species have been increasingly associated with intestinal disorders in pigs. This case report describes the first diagnosis of a “Brachyspira hampsonii” infection in European pigs. In a routine quarantine monitoring protocol, two gilts were presented for necropsy, in which soft watery non-haemorrhagic colonic content was found. Microbial culture from the colonic content and from faecal samples revealed the presence of strongly haemolytic, ring-phenomenon positive spirochetes indicative for Brachyspira hyodysenteriae. A diagnostic commercial PCR could not confirm the presence of B. hyodysenteriae. Phenotypic characterisation and PCRs targeting the 16S rRNA, 23S rRNA, nox, hlyA and tlyA genes of different swine-related Brachyspira spp. were performed. Phylogenetic analysis of sequences of the partial nox and 16S rRNA genes and multi locus sequence typing demonstrated that the isolates in this case were “B. hampsonii” isolates. This case report shows that the diagnosis of infections caused by new, emerging Brachyspira species is not self-evident and that the combination of microbial culture and PCR is recommended, completed with more extensive genotyping if necessary.
Keywords
swine dysentery, Brachyspira hampsonii, Brachyspira, haemolysis, INTESTINAL SPIROCHETOSIS, TREPONEMA-HYODYSENTERIAE, SP-NOV, SEQUENCE, PILOSICOLI, MURDOCHII, GENE, IDENTIFICATION, INTERMEDIA, VIRULENCE

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Citation

Please use this url to cite or link to this publication:

Chicago
Mahu, Maxime, Ellen de Jong, Nele De Pauw, Lien Vande Maele, Virginie Vandenbroucke, Tamara Vandersmissen, Cora Miry, et al. 2014. “First Isolation of ‘Brachyspira Hampsonii’ from Pigs in Europe.” Veterinary Record 174 (2).
APA
Mahu, M., de Jong, E., De Pauw, N., Vande Maele, L., Vandenbroucke, V., Vandersmissen, T., Miry, C., et al. (2014). First isolation of “Brachyspira hampsonii” from pigs in Europe. VETERINARY RECORD, 174(2).
Vancouver
1.
Mahu M, de Jong E, De Pauw N, Vande Maele L, Vandenbroucke V, Vandersmissen T, et al. First isolation of “Brachyspira hampsonii” from pigs in Europe. VETERINARY RECORD. 2014;174(2).
MLA
Mahu, Maxime, Ellen de Jong, Nele De Pauw, et al. “First Isolation of ‘Brachyspira Hampsonii’ from Pigs in Europe.” VETERINARY RECORD 174.2 (2014): n. pag. Print.
@article{4213166,
  abstract     = {Swine dysentery in Europe is classically attributed to Brachyspira hyodysenteriae. However, other Brachyspira species have been increasingly associated with intestinal disorders in pigs. This case report describes the first diagnosis of a {\textquotedblleft}Brachyspira hampsonii{\textquotedblright} infection in European pigs. In a routine quarantine monitoring protocol, two gilts were presented for necropsy, in which soft watery non-haemorrhagic colonic content was found. Microbial culture from the colonic content and from faecal samples revealed the presence of strongly haemolytic, ring-phenomenon positive spirochetes indicative for Brachyspira hyodysenteriae. A diagnostic commercial PCR could not confirm the presence of B. hyodysenteriae. Phenotypic characterisation and PCRs targeting the 16S rRNA, 23S rRNA, nox, hlyA and tlyA genes of different swine-related Brachyspira spp. were performed. Phylogenetic analysis of sequences of the partial nox and 16S rRNA genes and multi locus sequence typing demonstrated that the isolates in this case were {\textquotedblleft}B. hampsonii{\textquotedblright} isolates. This case report shows that the diagnosis of infections caused by new, emerging Brachyspira species is not self-evident and that the combination of microbial culture and PCR is recommended, completed with more extensive genotyping if necessary.},
  articleno    = {47},
  author       = {Mahu, Maxime and de Jong, Ellen and De Pauw, Nele and Vande Maele, Lien and Vandenbroucke, Virginie and Vandersmissen, Tamara and Miry, Cora and Pasmans, Frank and Haesebrouck, Freddy and Martel, An and Boyen, Filip},
  issn         = {0042-4900},
  journal      = {VETERINARY RECORD},
  language     = {eng},
  number       = {2},
  pages        = {4},
  title        = {First isolation of 'Brachyspira hampsonii' from pigs in Europe},
  url          = {http://dx.doi.org/10.1136/vr.101868},
  volume       = {174},
  year         = {2014},
}

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