Ghent University Academic Bibliography

Advanced

Efficient ROSA26-based conditional and/or inducible transgenesis using RMCE-compatible F1 hybrid mouse embryonic stem cells

Lieven Haenebalcke, Steven Goossens UGent, Michaël Naessens UGent, Natascha Kruse, Morvarid Farhang Ghahremani, Sona Bartunkova UGent, Katharina Haigh UGent, Tim Pieters UGent, Pieterjan Dierickx UGent, Benjamin Drogat UGent, et al. (2013) STEM CELL REVIEWS AND REPORTS. 9(6). p.774-785
abstract
The conditional Cre/loxP system and/or the doxycycline (Dox) inducible Tet-on/off system are widely used in mouse transgenesis but often require time consuming, inefficient cloning/screening steps and extensive mouse breeding strategies. We have therefore developed a highly efficient Gateway- and recombinase-mediated cassette exchange (RMCE)-compatible system to target conditional and/or inducible constructs to the ROSA26 locus of F1 hybrid Bl6/129 ESCs, called G4 ROSALUC ESCs. By combining the Cre/loxP system with or without the inducible Tet-on system using Gateway cloning, we can rapidly generate spatial and/or temporal controllable gain-of-function constructs that can be targeted to the RMCE-compatible ROSA26 locus of the G4 ROSALUC ESCs with efficiencies close to 100 %. These novel ESC-based technologies allow for the creation of multiple gain-of-function conditional and/or inducible transgenic ESC clones and mouse lines in a highly efficient and locus specific manner. Importantly, incorporating insulator sequences into the Dox-inducible vector system resulted in robust, stable transgene expression in undifferentiated ESCs but could not fully overcome transgene mosaicism in the differentiated state.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ROSA26, Recombinase mediated cassette exchange (RMCE), SITE-SPECIFIC INTEGRATION, GENE-EXPRESSION, IN-VIVO, ONCOGENE ADDICTION, RNA INTERFERENCE, CHROMOSOMAL LOCI, ES CELLS, MICE, SYSTEM, Gateway technology, Mouse embryonic stem cells, Insulators, GREEN FLUORESCENT PROTEIN, Conditional/Inducible (COIN)
journal title
STEM CELL REVIEWS AND REPORTS
Stem Cell Rev. Rep.
volume
9
issue
6
pages
774 - 785
Web of Science type
Article
Web of Science id
000327386500004
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
3.214 (2013)
JCR rank
38/124 (2013)
JCR quartile
2 (2013)
ISSN
1550-8943
DOI
10.1007/s12015-013-9458-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4213018
handle
http://hdl.handle.net/1854/LU-4213018
date created
2013-12-19 12:29:28
date last changed
2016-12-19 15:44:32
@article{4213018,
  abstract     = {The conditional Cre/loxP system and/or the doxycycline (Dox) inducible Tet-on/off system are widely used in mouse transgenesis but often require time consuming, inefficient cloning/screening steps and extensive mouse breeding strategies. We have therefore developed a highly efficient Gateway- and recombinase-mediated cassette exchange (RMCE)-compatible system to target conditional and/or inducible constructs to the ROSA26 locus of F1 hybrid Bl6/129 ESCs, called G4 ROSALUC ESCs. By combining the Cre/loxP system with or without the inducible Tet-on system using Gateway cloning, we can rapidly generate spatial and/or temporal controllable gain-of-function constructs that can be targeted to the RMCE-compatible ROSA26 locus of the G4 ROSALUC ESCs with efficiencies close to 100 \%. These novel ESC-based technologies allow for the creation of multiple gain-of-function conditional and/or inducible transgenic ESC clones and mouse lines in a highly efficient and locus specific manner. Importantly, incorporating insulator sequences into the Dox-inducible vector system resulted in robust, stable transgene expression in undifferentiated ESCs but could not fully overcome transgene mosaicism in the differentiated state.},
  author       = {Haenebalcke, Lieven and Goossens, Steven and Naessens, Micha{\"e}l and Kruse, Natascha and Farhang Ghahremani, Morvarid and Bartunkova, Sona and Haigh, Katharina and Pieters, Tim and Dierickx, Pieterjan and Drogat, Benjamin and Nyabi, Omar and Wirth, Dagmar and Haigh, Jody},
  issn         = {1550-8943},
  journal      = {STEM CELL REVIEWS AND REPORTS},
  keyword      = {ROSA26,Recombinase mediated cassette exchange (RMCE),SITE-SPECIFIC INTEGRATION,GENE-EXPRESSION,IN-VIVO,ONCOGENE ADDICTION,RNA INTERFERENCE,CHROMOSOMAL LOCI,ES CELLS,MICE,SYSTEM,Gateway technology,Mouse embryonic stem cells,Insulators,GREEN FLUORESCENT PROTEIN,Conditional/Inducible (COIN)},
  language     = {eng},
  number       = {6},
  pages        = {774--785},
  title        = {Efficient ROSA26-based conditional and/or inducible transgenesis using RMCE-compatible F1 hybrid mouse embryonic stem cells},
  url          = {http://dx.doi.org/10.1007/s12015-013-9458-z},
  volume       = {9},
  year         = {2013},
}

Chicago
Haenebalcke, Lieven, Steven Goossens, Michaël Naessens, Natascha Kruse, Morvarid Farhang Ghahremani, Sona Bartunkova, Katharina Haigh, et al. 2013. “Efficient ROSA26-based Conditional And/or Inducible Transgenesis Using RMCE-compatible F1 Hybrid Mouse Embryonic Stem Cells.” Stem Cell Reviews and Reports 9 (6): 774–785.
APA
Haenebalcke, L., Goossens, S., Naessens, M., Kruse, N., Farhang Ghahremani, M., Bartunkova, S., Haigh, K., et al. (2013). Efficient ROSA26-based conditional and/or inducible transgenesis using RMCE-compatible F1 hybrid mouse embryonic stem cells. STEM CELL REVIEWS AND REPORTS, 9(6), 774–785.
Vancouver
1.
Haenebalcke L, Goossens S, Naessens M, Kruse N, Farhang Ghahremani M, Bartunkova S, et al. Efficient ROSA26-based conditional and/or inducible transgenesis using RMCE-compatible F1 hybrid mouse embryonic stem cells. STEM CELL REVIEWS AND REPORTS. 2013;9(6):774–85.
MLA
Haenebalcke, Lieven, Steven Goossens, Michaël Naessens, et al. “Efficient ROSA26-based Conditional And/or Inducible Transgenesis Using RMCE-compatible F1 Hybrid Mouse Embryonic Stem Cells.” STEM CELL REVIEWS AND REPORTS 9.6 (2013): 774–785. Print.