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Brown adipose tissue blood flow and mass in obesity: a contrast ultrasound study in mice

Maëva Clerte, David M Baron, Peter Brouckaert UGent, Laura Ernande, Michael J Raher, Aidan W Flynn, Michael H Picard, Kenneth D Bloch, Emmanuel S Buys and Marielle Scherrer-Crosbie (2013) JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY. 26(12). p.1465-1473
abstract
Background: When activated by the sympathetic nervous system, brown adipose tissue (BAT) increases energy expenditure to produce heat. Augmenting BAT mass or increasing BAT activation could potentially be used to decrease obesity. Noninvasive methods to detect and monitor BAT mass are needed. Contrast ultrasound can estimate BAT blood flow and is able to measure the perfused volume of an organ and thus its mass. The objective of this study was to evaluate whether contrast ultrasound could characterize BAT mass in two mouse models of obesity: wild-type mice fed a high-fat diet and mutant db/db mice. Methods: Contrast ultrasound of BAT (Definity 2 mu L/min; 14-MHz linear probe) was performed before and after stimulation of BAT with norepinephrine (NE). BAT replenishment curves were obtained, and blood flow was estimated by the product of the curve's plateau and slope. Additionally, consecutive two-dimensional images of perfused BAT were acquired at 1-mmintervals after stimulation with NE and used to assess BAT volume and mass. Results: BAT blood flow increased after NE infusion in all mice studied. Blood flow response to NE was similar in wild-type mice fed either a low-fat diet or a high-fat diet. BAT blood flow was lower in db/db mice than in wild-type mice (P = .02). Contrast ultrasound-derived BAT mass was correlated with BAT mass obtained at necropsy (R-2 = 0.83, P < .001). BAT mass was higher in mice fed a high-fat diet than in those fed a low-fat diet. Conclusions: Contrast ultrasound can be used to estimate BAT mass in mice when BAT vascularization is not significantly impaired. This noninvasive technique may potentially allow the serial evaluation of therapies designed to augment BAT mass.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Contrast ultrasound, db/db mice, Imaging, Obesity, Brown adipose tissue, DIET-INDUCED THERMOGENESIS, LEFT-VENTRICULAR MASS, COLD-ACCLIMATED RATS, ECHOCARDIOGRAPHIC-ASSESSMENT, NONSHIVERING THERMOGENESIS, MYOCARDIAL-INFARCTION, CAFETERIA DIET, ENERGY-BALANCE, MOUSE, NORADRENALINE
journal title
JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY
J. Am. Soc. Echocardiogr.
volume
26
issue
12
pages
1465 - 1473
Web of Science type
Article
Web of Science id
000327536800015
JCR category
CARDIAC & CARDIOVASCULAR SYSTEMS
JCR impact factor
3.987 (2013)
JCR rank
30/125 (2013)
JCR quartile
1 (2013)
ISSN
0894-7317
DOI
10.1016/j.echo.2013.07.015
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4212947
handle
http://hdl.handle.net/1854/LU-4212947
date created
2013-12-19 12:13:07
date last changed
2016-12-19 15:37:56
@article{4212947,
  abstract     = {Background: When activated by the sympathetic nervous system, brown adipose tissue (BAT) increases energy expenditure to produce heat. Augmenting BAT mass or increasing BAT activation could potentially be used to decrease obesity. Noninvasive methods to detect and monitor BAT mass are needed. Contrast ultrasound can estimate BAT blood flow and is able to measure the perfused volume of an organ and thus its mass. The objective of this study was to evaluate whether contrast ultrasound could characterize BAT mass in two mouse models of obesity: wild-type mice fed a high-fat diet and mutant db/db mice.
Methods: Contrast ultrasound of BAT (Definity 2 mu L/min; 14-MHz linear probe) was performed before and after stimulation of BAT with norepinephrine (NE). BAT replenishment curves were obtained, and blood flow was estimated by the product of the curve's plateau and slope. Additionally, consecutive two-dimensional images of perfused BAT were acquired at 1-mmintervals after stimulation with NE and used to assess BAT volume and mass.
Results: BAT blood flow increased after NE infusion in all mice studied. Blood flow response to NE was similar in wild-type mice fed either a low-fat diet or a high-fat diet. BAT blood flow was lower in db/db mice than in wild-type mice (P = .02). Contrast ultrasound-derived BAT mass was correlated with BAT mass obtained at necropsy (R-2 = 0.83, P {\textlangle} .001). BAT mass was higher in mice fed a high-fat diet than in those fed a low-fat diet.
Conclusions: Contrast ultrasound can be used to estimate BAT mass in mice when BAT vascularization is not significantly impaired. This noninvasive technique may potentially allow the serial evaluation of therapies designed to augment BAT mass.},
  author       = {Clerte, Ma{\"e}va and Baron, David M and Brouckaert, Peter and Ernande, Laura and Raher, Michael J and Flynn, Aidan W and Picard, Michael H and Bloch, Kenneth D and Buys, Emmanuel S and Scherrer-Crosbie, Marielle},
  issn         = {0894-7317},
  journal      = {JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY},
  keyword      = {Contrast ultrasound,db/db mice,Imaging,Obesity,Brown adipose tissue,DIET-INDUCED THERMOGENESIS,LEFT-VENTRICULAR MASS,COLD-ACCLIMATED RATS,ECHOCARDIOGRAPHIC-ASSESSMENT,NONSHIVERING THERMOGENESIS,MYOCARDIAL-INFARCTION,CAFETERIA DIET,ENERGY-BALANCE,MOUSE,NORADRENALINE},
  language     = {eng},
  number       = {12},
  pages        = {1465--1473},
  title        = {Brown adipose tissue blood flow and mass in obesity: a contrast ultrasound study in mice},
  url          = {http://dx.doi.org/10.1016/j.echo.2013.07.015},
  volume       = {26},
  year         = {2013},
}

Chicago
Clerte, Maëva, David M Baron, Peter Brouckaert, Laura Ernande, Michael J Raher, Aidan W Flynn, Michael H Picard, Kenneth D Bloch, Emmanuel S Buys, and Marielle Scherrer-Crosbie. 2013. “Brown Adipose Tissue Blood Flow and Mass in Obesity: a Contrast Ultrasound Study in Mice.” Journal of the American Society of Echocardiography 26 (12): 1465–1473.
APA
Clerte, M., Baron, D. M., Brouckaert, P., Ernande, L., Raher, M. J., Flynn, A. W., Picard, M. H., et al. (2013). Brown adipose tissue blood flow and mass in obesity: a contrast ultrasound study in mice. JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY, 26(12), 1465–1473.
Vancouver
1.
Clerte M, Baron DM, Brouckaert P, Ernande L, Raher MJ, Flynn AW, et al. Brown adipose tissue blood flow and mass in obesity: a contrast ultrasound study in mice. JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY. 2013;26(12):1465–73.
MLA
Clerte, Maëva, David M Baron, Peter Brouckaert, et al. “Brown Adipose Tissue Blood Flow and Mass in Obesity: a Contrast Ultrasound Study in Mice.” JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY 26.12 (2013): 1465–1473. Print.