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Rapid manufacturing of collagen scaffolds for cardiac tissue engineering

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Abstract
This manuscript describes the development of a collagen moulding method for the reproducible rapid manufacturing of storable thin collagen leaflets up to 50 m in thickness, which are intended for use as cardiac patches. The fabricated collagen leaflets are seeded with bone marrow mesenchymal stem cells and kept in culture up to 7 days, after which a high percentage of cell viability is observed, as well as an adherent dense multilayer of cells. The mechanical properties of the leaflets, which are determined in a flexural indentation experiment, are observed to be inferior to those of the natural aortic valve cusp. As such, the parts created with the collagen moulding method are considered very suitable for further research to use them as cardiac patches but will require further improvement of their structural integrity for application as valve cusp scaffolds.
Keywords
MESENCHYMAL STEM-CELLS, REGENERATION, HEART-VALVES, ARCHITECTURE, ADHESION, MATRICES

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Chicago
Ragaert, Kim, Stieven Van de Velde, Ludwig Cardon, Filip De Somer, Guido Van Nooten, and Pamela Somers. 2014. “Rapid Manufacturing of Collagen Scaffolds for Cardiac Tissue Engineering.” In High Value Manufacturing : Advanced Research in Virtual and Rapid Prototyping, ed. PJD Bartolo, ACS DeLemos, AMH Pereira, AJD Mateus, C Ramos, C DosSantos, D Oliveira, et al., 137–142. Boca Raton, FL, USA: CRC Press.
APA
Ragaert, K., Van de Velde, S., Cardon, L., De Somer, F., Van Nooten, G., & Somers, P. (2014). Rapid manufacturing of collagen scaffolds for cardiac tissue engineering. In PJD Bartolo, A. DeLemos, A. Pereira, A. Mateus, C. Ramos, C. DosSantos, D. Oliveira, et al. (Eds.), High value manufacturing : advanced research in virtual and rapid prototyping (pp. 137–142). Presented at the 6th International conference on Advanced Research in Virtual and Physical Prototyping (VRatP), Boca Raton, FL, USA: CRC Press.
Vancouver
1.
Ragaert K, Van de Velde S, Cardon L, De Somer F, Van Nooten G, Somers P. Rapid manufacturing of collagen scaffolds for cardiac tissue engineering. In: Bartolo P, DeLemos A, Pereira A, Mateus A, Ramos C, DosSantos C, et al., editors. High value manufacturing : advanced research in virtual and rapid prototyping. Boca Raton, FL, USA: CRC Press; 2014. p. 137–42.
MLA
Ragaert, Kim, Stieven Van de Velde, Ludwig Cardon, et al. “Rapid Manufacturing of Collagen Scaffolds for Cardiac Tissue Engineering.” High Value Manufacturing : Advanced Research in Virtual and Rapid Prototyping. Ed. PJD Bartolo et al. Boca Raton, FL, USA: CRC Press, 2014. 137–142. Print.
@inproceedings{4210869,
  abstract     = {This manuscript describes the development of a collagen moulding method for the reproducible rapid manufacturing of storable thin collagen leaflets up to 50 m in thickness, which are intended for use as cardiac patches. The fabricated collagen leaflets are seeded with bone marrow mesenchymal stem cells and kept in culture up to 7 days, after which a high percentage of cell viability is observed, as well as an adherent dense multilayer of cells. The mechanical properties of the leaflets, which are determined in a flexural indentation experiment, are observed to be inferior to those of the natural aortic valve cusp. As such, the parts created with the collagen moulding method are considered very suitable for further research to use them as cardiac patches but will require further improvement of their structural integrity for application as valve cusp scaffolds.},
  author       = {Ragaert, Kim and Van de Velde, Stieven and Cardon, Ludwig and De Somer, Filip and Van Nooten, Guido and Somers, Pamela},
  booktitle    = {High value manufacturing : advanced research in virtual and rapid prototyping},
  editor       = {Bartolo, PJD and DeLemos, ACS and Pereira, AMH and Mateus, AJD and Ramos, C and DosSantos, C and Oliveira, D and Pinto, E and Craveiro, F and Barolo, HMCDFTG and Almeia, HD and Sousa, I and Matias, JM and Durao, L and Gaspar, M and Alves, NMF and Carreira, P and Ferreira, T and Marques, T},
  isbn         = {9781315817415},
  keyword      = {MESENCHYMAL STEM-CELLS,REGENERATION,HEART-VALVES,ARCHITECTURE,ADHESION,MATRICES},
  language     = {eng},
  location     = {Leiria, Portugal},
  pages        = {137--142},
  publisher    = {CRC Press},
  title        = {Rapid manufacturing of collagen scaffolds for cardiac tissue engineering},
  year         = {2014},
}

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