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Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis

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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation.
Keywords
DISSECTION, EVOLUTION, TRANSCRIPTOME, ROOT, THALIANA, BINDING PROTEIN-1, POLAR TRANSPORT, PLANTS, IDENTIFICATION, GENE

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Chicago
Ranocha, Philippe, Oana Dima, Réka Nagy, Judith Felten, Claire Corratgé-Faillie, Ondřej Novák, Kris Morreel, et al. 2013. “Arabidopsis WAT1 Is a Vacuolar Auxin Transport Facilitator Required for Auxin Homoeostasis.” Nature Communications 4.
APA
Ranocha, P., Dima, O., Nagy, R., Felten, J., Corratgé-Faillie, C., Novák, O., Morreel, K., et al. (2013). Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis. NATURE COMMUNICATIONS, 4.
Vancouver
1.
Ranocha P, Dima O, Nagy R, Felten J, Corratgé-Faillie C, Novák O, et al. Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis. NATURE COMMUNICATIONS. 2013;4.
MLA
Ranocha, Philippe, Oana Dima, Réka Nagy, et al. “Arabidopsis WAT1 Is a Vacuolar Auxin Transport Facilitator Required for Auxin Homoeostasis.” NATURE COMMUNICATIONS 4 (2013): n. pag. Print.
@article{4206503,
  abstract     = {The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation.},
  articleno    = {2625},
  author       = {Ranocha, Philippe and Dima, Oana and Nagy, R{\'e}ka and Felten, Judith and Corratg{\'e}-Faillie, Claire and Nov{\'a}k, Ond\v{r}ej and Morreel, Kris and Lacombe, Beno{\^i}t and Martinez, Yves and Pfrunder, Stephanie and Jin, Xu and Renou, Jean-Pierre and Thibaud, Jean-Baptiste and Ljung, Karin and Fischer, Uus and Martinoia, Enrico and Boerjan, Wout and Goffner, Deborah},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keyword      = {DISSECTION,EVOLUTION,TRANSCRIPTOME,ROOT,THALIANA,BINDING PROTEIN-1,POLAR TRANSPORT,PLANTS,IDENTIFICATION,GENE},
  language     = {eng},
  pages        = {9},
  title        = {Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis},
  url          = {http://dx.doi.org/10.1038/ncomms3625},
  volume       = {4},
  year         = {2013},
}

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