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Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis

Philippe Ranocha, Oana Dima, Réka Nagy, Judith Felten, Claire Corratgé-Faillie, Ondřej Novák, Kris Morreel UGent, Benoît Lacombe, Yves Martinez, Stephanie Pfrunder, et al. (2013) NATURE COMMUNICATIONS. 4.
abstract
The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DISSECTION, EVOLUTION, TRANSCRIPTOME, ROOT, THALIANA, BINDING PROTEIN-1, POLAR TRANSPORT, PLANTS, IDENTIFICATION, GENE
journal title
NATURE COMMUNICATIONS
Nat. Commun.
volume
4
article number
2625
pages
9 pages
Web of Science type
Article
Web of Science id
000326472200014
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
10.742 (2013)
JCR rank
3/55 (2013)
JCR quartile
1 (2013)
ISSN
2041-1723
DOI
10.1038/ncomms3625
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
4206503
handle
http://hdl.handle.net/1854/LU-4206503
date created
2013-12-11 13:28:37
date last changed
2017-05-04 14:15:00
@article{4206503,
  abstract     = {The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation.},
  articleno    = {2625},
  author       = {Ranocha, Philippe and Dima, Oana and Nagy, R{\'e}ka and Felten, Judith and Corratg{\'e}-Faillie, Claire and Nov{\'a}k, Ond\v{r}ej and Morreel, Kris and Lacombe, Beno{\^i}t and Martinez, Yves and Pfrunder, Stephanie and Jin, Xu and Renou, Jean-Pierre and Thibaud, Jean-Baptiste and Ljung, Karin and Fischer, Uus and Martinoia, Enrico and Boerjan, Wout and Goffner, Deborah},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keyword      = {DISSECTION,EVOLUTION,TRANSCRIPTOME,ROOT,THALIANA,BINDING PROTEIN-1,POLAR TRANSPORT,PLANTS,IDENTIFICATION,GENE},
  language     = {eng},
  pages        = {9},
  title        = {Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis},
  url          = {http://dx.doi.org/10.1038/ncomms3625},
  volume       = {4},
  year         = {2013},
}

Chicago
Ranocha, Philippe, Oana Dima, Réka Nagy, Judith Felten, Claire Corratgé-Faillie, Ondřej Novák, Kris Morreel, et al. 2013. “Arabidopsis WAT1 Is a Vacuolar Auxin Transport Facilitator Required for Auxin Homoeostasis.” Nature Communications 4.
APA
Ranocha, P., Dima, O., Nagy, R., Felten, J., Corratgé-Faillie, C., Novák, O., Morreel, K., et al. (2013). Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis. NATURE COMMUNICATIONS, 4.
Vancouver
1.
Ranocha P, Dima O, Nagy R, Felten J, Corratgé-Faillie C, Novák O, et al. Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis. NATURE COMMUNICATIONS. 2013;4.
MLA
Ranocha, Philippe, Oana Dima, Réka Nagy, et al. “Arabidopsis WAT1 Is a Vacuolar Auxin Transport Facilitator Required for Auxin Homoeostasis.” NATURE COMMUNICATIONS 4 (2013): n. pag. Print.