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Comparison of HPLC methods for the determination of amino sugars in soil hydrolysates

(2013) ANALYTICAL LETTERS. 46(14). p.2145-2164
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Abstract
A study on the suitability of chromatographic techniques such as high performance anion exchange chromatography (HPAEC) with fluorescence detection (FL) and pulsed amperometric detection (PAD) and reversed phase (RP) chromatography for the determination of galactosamine, glucosamine, mannosamine, and muramic acid in soil hydrolysates was carried out. The reversed phase fluorescence method was rapid, provided good validation parameters, and employed relatively inexpensive instrumentation. The HPAEC methods had slightly higher limits of quantification, 0.6-5.0 mu molL(-1) (HPAEC-FL) and 1.0-10.0 mu molL(-1) (HPAEC-PAD), compared to the reversed phase fluorescence method (0.5-5.0 mu molL(-1)). Various sample pretreatment methods and chromatographic methods were investigated and the advantages and disadvantages of the HPLC methods are discussed.
Keywords
Derivatization, Amino sugars, HPAEC, HPLC, Microbial residues, OPA, PAD, Soil analysis, ANION-EXCHANGE CHROMATOGRAPHY, PULSED AMPEROMETRIC DETECTION, PERFORMANCE LIQUID-CHROMATOGRAPHY, ION-EXCLUSION CHROMATOGRAPHY, RATIO MASS-SPECTROMETRY, MURAMIC ACID, DELTA-C-13 ANALYSIS, PUSHING AGENTS, FLUORESCENCE, GLUCOSAMINE

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Citation

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Chicago
Indorf, Caroline, Samuel Bodé, Pascal Boeckx, Jens Dyckmans, Axel Meyer, Klaus Fischer, and Rainer Georg Joergensen. 2013. “Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates.” Analytical Letters 46 (14): 2145–2164.
APA
Indorf, C., Bodé, S., Boeckx, P., Dyckmans, J., Meyer, A., Fischer, K., & Joergensen, R. G. (2013). Comparison of HPLC methods for the determination of amino sugars in soil hydrolysates. ANALYTICAL LETTERS, 46(14), 2145–2164.
Vancouver
1.
Indorf C, Bodé S, Boeckx P, Dyckmans J, Meyer A, Fischer K, et al. Comparison of HPLC methods for the determination of amino sugars in soil hydrolysates. ANALYTICAL LETTERS. 2013;46(14):2145–64.
MLA
Indorf, Caroline, Samuel Bodé, Pascal Boeckx, et al. “Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates.” ANALYTICAL LETTERS 46.14 (2013): 2145–2164. Print.
@article{4206210,
  abstract     = {A study on the suitability of chromatographic techniques such as high performance anion exchange chromatography (HPAEC) with fluorescence detection (FL) and pulsed amperometric detection (PAD) and reversed phase (RP) chromatography for the determination of galactosamine, glucosamine, mannosamine, and muramic acid in soil hydrolysates was carried out. The reversed phase fluorescence method was rapid, provided good validation parameters, and employed relatively inexpensive instrumentation. The HPAEC methods had slightly higher limits of quantification, 0.6-5.0 mu molL(-1) (HPAEC-FL) and 1.0-10.0 mu molL(-1) (HPAEC-PAD), compared to the reversed phase fluorescence method (0.5-5.0 mu molL(-1)). Various sample pretreatment methods and chromatographic methods were investigated and the advantages and disadvantages of the HPLC methods are discussed.},
  author       = {Indorf, Caroline and Bod{\'e}, Samuel and Boeckx, Pascal and Dyckmans, Jens and Meyer, Axel and Fischer, Klaus and Joergensen, Rainer Georg},
  issn         = {0003-2719},
  journal      = {ANALYTICAL LETTERS},
  keyword      = {Derivatization,Amino sugars,HPAEC,HPLC,Microbial residues,OPA,PAD,Soil analysis,ANION-EXCHANGE CHROMATOGRAPHY,PULSED AMPEROMETRIC DETECTION,PERFORMANCE LIQUID-CHROMATOGRAPHY,ION-EXCLUSION CHROMATOGRAPHY,RATIO MASS-SPECTROMETRY,MURAMIC ACID,DELTA-C-13 ANALYSIS,PUSHING AGENTS,FLUORESCENCE,GLUCOSAMINE},
  language     = {eng},
  number       = {14},
  pages        = {2145--2164},
  title        = {Comparison of HPLC methods for the determination of amino sugars in soil hydrolysates},
  url          = {http://dx.doi.org/10.1080/00032719.2013.796558},
  volume       = {46},
  year         = {2013},
}

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