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Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure

(2014) BIODEGRADATION. 25(4). p.493-504
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Abstract
This study aimed at monitoring the dynamics of phylogenetic and catabolic genes of a dechlorinating enrichment culture before, during, and after complete dechlorination of chlorinated compounds. More specifically, the effect of 40 mu M trichloroethene (TCE) and 5.6 mM lactate on the gene abundance and activity of an enrichment culture was investigated for 40 days. Although tceA and vcrA gene copy numbers were relatively stable in DNA extracts over time, tceA and vcrA mRNA abundances were up-regulated from undetectable levels to 2.96 x and 6.33 x 10(4) transcripts/mL, respectively, only after exposure to TCE and lactate. While tceA gene transcripts decreased over time with TCE dechlorination, the vcrA gene was expressed steadily even when the concentration of vinyl chloride was at undetectable levels. In addition, ratios between catabolic and phylogenetic genes indicated that tceA and vcrA gene carrying organisms dechlorinated TCE and its produced daughter products, while vcrA gene was mainly responsible for the dechlorination of the lower VC concentrations in a later stage of degradation.
Keywords
RDase genes, Dehalococcoides spp., ATP activity, RNA, Dynamics, REDUCTIVE DEHALOGENASE GENES, 16S RIBOSOMAL-RNA, REAL-TIME PCR, VINYL-CHLORIDE, CONTAMINATED GROUNDWATER, ENRICHMENT CULTURE, SP STRAIN, TRICHLOROETHENE, EXPRESSION, ETHENE

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Chicago
Dogan Subasi, Eylem, Leen Bastiaens, Natalie Leys, Nico Boon, and Winnie Dejonghe. 2014. “Quantitative and Functional Dynamics of Dehalococcoides Spp. and Its tceA and vcrA Genes Under TCE Exposure.” Biodegradation 25 (4): 493–504.
APA
Dogan Subasi, E., Bastiaens, L., Leys, N., Boon, N., & Dejonghe, W. (2014). Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure. BIODEGRADATION, 25(4), 493–504.
Vancouver
1.
Dogan Subasi E, Bastiaens L, Leys N, Boon N, Dejonghe W. Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure. BIODEGRADATION. 2014;25(4):493–504.
MLA
Dogan Subasi, Eylem, Leen Bastiaens, Natalie Leys, et al. “Quantitative and Functional Dynamics of Dehalococcoides Spp. and Its tceA and vcrA Genes Under TCE Exposure.” BIODEGRADATION 25.4 (2014): 493–504. Print.
@article{4195256,
  abstract     = {This study aimed at monitoring the dynamics of phylogenetic and catabolic genes of a dechlorinating enrichment culture before, during, and after complete dechlorination of chlorinated compounds. More specifically, the effect of 40 mu M trichloroethene (TCE) and 5.6 mM lactate on the gene abundance and activity of an enrichment culture was investigated for 40 days. Although tceA and vcrA gene copy numbers were relatively stable in DNA extracts over time, tceA and vcrA mRNA abundances were up-regulated from undetectable levels to 2.96 x and 6.33 x 10(4) transcripts/mL, respectively, only after exposure to TCE and lactate. While tceA gene transcripts decreased over time with TCE dechlorination, the vcrA gene was expressed steadily even when the concentration of vinyl chloride was at undetectable levels. In addition, ratios between catabolic and phylogenetic genes indicated that tceA and vcrA gene carrying organisms dechlorinated TCE and its produced daughter products, while vcrA gene was mainly responsible for the dechlorination of the lower VC concentrations in a later stage of degradation.},
  author       = {Dogan Subasi, Eylem and Bastiaens, Leen and Leys, Natalie and Boon, Nico and Dejonghe, Winnie},
  issn         = {0923-9820},
  journal      = {BIODEGRADATION},
  keyword      = {RDase genes,Dehalococcoides spp.,ATP activity,RNA,Dynamics,REDUCTIVE DEHALOGENASE GENES,16S RIBOSOMAL-RNA,REAL-TIME PCR,VINYL-CHLORIDE,CONTAMINATED GROUNDWATER,ENRICHMENT CULTURE,SP STRAIN,TRICHLOROETHENE,EXPRESSION,ETHENE},
  language     = {eng},
  number       = {4},
  pages        = {493--504},
  title        = {Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure},
  url          = {http://dx.doi.org/10.1007/s10532-013-9676-8},
  volume       = {25},
  year         = {2014},
}

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