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Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure

Eylem Dogan Subasi, Leen Bastiaens, Natalie Leys, Nico Boon UGent and Winnie Dejonghe (2014) BIODEGRADATION. 25(4). p.493-504
abstract
This study aimed at monitoring the dynamics of phylogenetic and catabolic genes of a dechlorinating enrichment culture before, during, and after complete dechlorination of chlorinated compounds. More specifically, the effect of 40 mu M trichloroethene (TCE) and 5.6 mM lactate on the gene abundance and activity of an enrichment culture was investigated for 40 days. Although tceA and vcrA gene copy numbers were relatively stable in DNA extracts over time, tceA and vcrA mRNA abundances were up-regulated from undetectable levels to 2.96 x and 6.33 x 10(4) transcripts/mL, respectively, only after exposure to TCE and lactate. While tceA gene transcripts decreased over time with TCE dechlorination, the vcrA gene was expressed steadily even when the concentration of vinyl chloride was at undetectable levels. In addition, ratios between catabolic and phylogenetic genes indicated that tceA and vcrA gene carrying organisms dechlorinated TCE and its produced daughter products, while vcrA gene was mainly responsible for the dechlorination of the lower VC concentrations in a later stage of degradation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RDase genes, Dehalococcoides spp., ATP activity, RNA, Dynamics, REDUCTIVE DEHALOGENASE GENES, 16S RIBOSOMAL-RNA, REAL-TIME PCR, VINYL-CHLORIDE, CONTAMINATED GROUNDWATER, ENRICHMENT CULTURE, SP STRAIN, TRICHLOROETHENE, EXPRESSION, ETHENE
journal title
BIODEGRADATION
Biodegradation
volume
25
issue
4
pages
493 - 504
Web of Science type
Article
Web of Science id
000337064200002
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.336 (2014)
JCR rank
73/163 (2014)
JCR quartile
2 (2014)
ISSN
0923-9820
DOI
10.1007/s10532-013-9676-8
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4195256
handle
http://hdl.handle.net/1854/LU-4195256
date created
2013-12-03 17:33:21
date last changed
2016-12-19 15:38:24
@article{4195256,
  abstract     = {This study aimed at monitoring the dynamics of phylogenetic and catabolic genes of a dechlorinating enrichment culture before, during, and after complete dechlorination of chlorinated compounds. More specifically, the effect of 40 mu M trichloroethene (TCE) and 5.6 mM lactate on the gene abundance and activity of an enrichment culture was investigated for 40 days. Although tceA and vcrA gene copy numbers were relatively stable in DNA extracts over time, tceA and vcrA mRNA abundances were up-regulated from undetectable levels to 2.96 x and 6.33 x 10(4) transcripts/mL, respectively, only after exposure to TCE and lactate. While tceA gene transcripts decreased over time with TCE dechlorination, the vcrA gene was expressed steadily even when the concentration of vinyl chloride was at undetectable levels. In addition, ratios between catabolic and phylogenetic genes indicated that tceA and vcrA gene carrying organisms dechlorinated TCE and its produced daughter products, while vcrA gene was mainly responsible for the dechlorination of the lower VC concentrations in a later stage of degradation.},
  author       = {Dogan Subasi, Eylem and Bastiaens, Leen and Leys, Natalie and Boon, Nico and Dejonghe, Winnie},
  issn         = {0923-9820},
  journal      = {BIODEGRADATION},
  keyword      = {RDase genes,Dehalococcoides spp.,ATP activity,RNA,Dynamics,REDUCTIVE DEHALOGENASE GENES,16S RIBOSOMAL-RNA,REAL-TIME PCR,VINYL-CHLORIDE,CONTAMINATED GROUNDWATER,ENRICHMENT CULTURE,SP STRAIN,TRICHLOROETHENE,EXPRESSION,ETHENE},
  language     = {eng},
  number       = {4},
  pages        = {493--504},
  title        = {Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure},
  url          = {http://dx.doi.org/10.1007/s10532-013-9676-8},
  volume       = {25},
  year         = {2014},
}

Chicago
Dogan Subasi, Eylem, Leen Bastiaens, Natalie Leys, Nico Boon, and Winnie Dejonghe. 2014. “Quantitative and Functional Dynamics of Dehalococcoides Spp. and Its tceA and vcrA Genes Under TCE Exposure.” Biodegradation 25 (4): 493–504.
APA
Dogan Subasi, E., Bastiaens, L., Leys, N., Boon, N., & Dejonghe, W. (2014). Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure. BIODEGRADATION, 25(4), 493–504.
Vancouver
1.
Dogan Subasi E, Bastiaens L, Leys N, Boon N, Dejonghe W. Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure. BIODEGRADATION. 2014;25(4):493–504.
MLA
Dogan Subasi, Eylem, Leen Bastiaens, Natalie Leys, et al. “Quantitative and Functional Dynamics of Dehalococcoides Spp. and Its tceA and vcrA Genes Under TCE Exposure.” BIODEGRADATION 25.4 (2014): 493–504. Print.