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Identification and characterization of a type III secretion system in Chlamydophila psittaci

Delphine Sylvie Anne Beeckman UGent, Tom Geens, Jean-Pierre Timmermans, Patric Van Oostveldt UGent and Daisy Vanrompay UGent (2008) VETERINARY RESEARCH. 39(3).
abstract
Chlamydiaceae are obligate intracellular Gram-negative bacteria replicating in vacuoles inside eukaryotic cells. It has been proven that most of them possess a type III secretion system (T3SS) allowing them to transfer effector molecules in the host cell. We examined the existence of a T3SS in Chlamydophila psittaci by studying the expression of three essential structural proteins SctW, SctC, and SctN, and one putative effector protein IncA. Immunofluorescence assays showed SctW and IncA to be associated with the bacteria and the inclusion membrane, while SctC and SctN were only localized to the bacteria itself. Immuno electron microscopy could confirm these results for SctW, IncA, and SctC. Unfortunately, SctN was not investigated with this technique. Additionally, we sequenced 14 full-length T3S genes (scc1, sctW, sctJ, sctL, sctR, sctS, scc2, copD1, sctN, sctQ, sctC, incA, ca037, and cadd) and examined the transcription of 26 Cp. psittaci T3S genes namely cluster 1 (scc1, sctW, sctV, sctU), cluster 2 (sctJ, sctL, sctR, sctS, sctT, scc2, copB1, copD1), cluster 3 (sctD, sctN, ca037, sctQ, pkn5, sctC) and non-clustered genes (incA, incC, scc3, copD2, cap1, tarp, ca530, cadd). The gene expression study indicated the T3S structural protein encoding genes to be transcribed from mid-cycle (12-18 h post infection (p.i.)) on. Genes encoding effector proteins and putative T3S related proteins were expressed early (1.5 h-8 h p.i.) or late (> 24 h p.i.) during the developmental cycle. We hereby provided evidence for the existence of a T3SS and possible effectors in avian Cp. psittaci.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RECRUITMENT, PHOSPHORYLATION, INCLUSION MEMBRANE, PNEUMONIAE, EXPRESSION, CELLS, AVIAN CHLAMYDIOSIS, GENOME SEQUENCE, TRANSLOCATED PROTEIN, CHLAMYDIA-TRACHOMATIS, chlamydia, type III secretion, Chlamydophila psittaci
journal title
VETERINARY RESEARCH
Vet. Res.
volume
39
issue
3
article_number
27
pages
18 pages
Web of Science type
Article
Web of Science id
000256116300005
JCR category
VETERINARY SCIENCES
JCR impact factor
3.06 (2008)
JCR rank
4/133 (2008)
JCR quartile
1 (2008)
ISSN
0928-4249
DOI
10.1051/vetres:2008002
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
419217
handle
http://hdl.handle.net/1854/LU-419217
date created
2008-06-17 08:22:00
date last changed
2010-09-09 16:16:20
@article{419217,
  abstract     = {Chlamydiaceae are obligate intracellular Gram-negative bacteria replicating in vacuoles inside eukaryotic cells. It has been proven that most of them possess a type III secretion system (T3SS) allowing them to transfer effector molecules in the host cell. We examined the existence of a T3SS in Chlamydophila psittaci by studying the expression of three essential structural proteins SctW, SctC, and SctN, and one putative effector protein IncA. Immunofluorescence assays showed SctW and IncA to be associated with the bacteria and the inclusion membrane, while SctC and SctN were only localized to the bacteria itself. Immuno electron microscopy could confirm these results for SctW, IncA, and SctC. Unfortunately, SctN was not investigated with this technique. Additionally, we sequenced 14 full-length T3S genes (scc1, sctW, sctJ, sctL, sctR, sctS, scc2, copD1, sctN, sctQ, sctC, incA, ca037, and cadd) and examined the transcription of 26 Cp. psittaci T3S genes namely cluster 1 (scc1, sctW, sctV, sctU), cluster 2 (sctJ, sctL, sctR, sctS, sctT, scc2, copB1, copD1), cluster 3 (sctD, sctN, ca037, sctQ, pkn5, sctC) and non-clustered genes (incA, incC, scc3, copD2, cap1, tarp, ca530, cadd). The gene expression study indicated the T3S structural protein encoding genes to be transcribed from mid-cycle (12-18 h post infection (p.i.)) on. Genes encoding effector proteins and putative T3S related proteins were expressed early (1.5 h-8 h p.i.) or late ({\textrangle} 24 h p.i.) during the developmental cycle. We hereby provided evidence for the existence of a T3SS and possible effectors in avian Cp. psittaci.},
  articleno    = {27},
  author       = {Beeckman, Delphine Sylvie Anne and Geens, Tom and Timmermans, Jean-Pierre and Van Oostveldt, Patric and Vanrompay, Daisy},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {RECRUITMENT,PHOSPHORYLATION,INCLUSION MEMBRANE,PNEUMONIAE,EXPRESSION,CELLS,AVIAN CHLAMYDIOSIS,GENOME SEQUENCE,TRANSLOCATED PROTEIN,CHLAMYDIA-TRACHOMATIS,chlamydia,type III secretion,Chlamydophila psittaci},
  language     = {eng},
  number       = {3},
  pages        = {18},
  title        = {Identification and characterization of a type III secretion system in Chlamydophila psittaci},
  url          = {http://dx.doi.org/10.1051/vetres:2008002},
  volume       = {39},
  year         = {2008},
}

Chicago
Beeckman, Delphine Sylvie Anne, Tom Geens, Jean-Pierre Timmermans, Patric Van Oostveldt, and Daisy Vanrompay. 2008. “Identification and Characterization of a Type III Secretion System in Chlamydophila Psittaci.” Veterinary Research 39 (3).
APA
Beeckman, D. S. A., Geens, T., Timmermans, J.-P., Van Oostveldt, P., & Vanrompay, D. (2008). Identification and characterization of a type III secretion system in Chlamydophila psittaci. VETERINARY RESEARCH, 39(3).
Vancouver
1.
Beeckman DSA, Geens T, Timmermans J-P, Van Oostveldt P, Vanrompay D. Identification and characterization of a type III secretion system in Chlamydophila psittaci. VETERINARY RESEARCH. 2008;39(3).
MLA
Beeckman, Delphine Sylvie Anne, Tom Geens, Jean-Pierre Timmermans, et al. “Identification and Characterization of a Type III Secretion System in Chlamydophila Psittaci.” VETERINARY RESEARCH 39.3 (2008): n. pag. Print.