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Identification and characterization of a type III secretion system in Chlamydophila psittaci

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Abstract
Chlamydiaceae are obligate intracellular Gram-negative bacteria replicating in vacuoles inside eukaryotic cells. It has been proven that most of them possess a type III secretion system (T3SS) allowing them to transfer effector molecules in the host cell. We examined the existence of a T3SS in Chlamydophila psittaci by studying the expression of three essential structural proteins SctW, SctC, and SctN, and one putative effector protein IncA. Immunofluorescence assays showed SctW and IncA to be associated with the bacteria and the inclusion membrane, while SctC and SctN were only localized to the bacteria itself. Immuno electron microscopy could confirm these results for SctW, IncA, and SctC. Unfortunately, SctN was not investigated with this technique. Additionally, we sequenced 14 full-length T3S genes (scc1, sctW, sctJ, sctL, sctR, sctS, scc2, copD1, sctN, sctQ, sctC, incA, ca037, and cadd) and examined the transcription of 26 Cp. psittaci T3S genes namely cluster 1 (scc1, sctW, sctV, sctU), cluster 2 (sctJ, sctL, sctR, sctS, sctT, scc2, copB1, copD1), cluster 3 (sctD, sctN, ca037, sctQ, pkn5, sctC) and non-clustered genes (incA, incC, scc3, copD2, cap1, tarp, ca530, cadd). The gene expression study indicated the T3S structural protein encoding genes to be transcribed from mid-cycle (12-18 h post infection (p.i.)) on. Genes encoding effector proteins and putative T3S related proteins were expressed early (1.5 h-8 h p.i.) or late (> 24 h p.i.) during the developmental cycle. We hereby provided evidence for the existence of a T3SS and possible effectors in avian Cp. psittaci.
Keywords
RECRUITMENT, PHOSPHORYLATION, INCLUSION MEMBRANE, PNEUMONIAE, EXPRESSION, CELLS, AVIAN CHLAMYDIOSIS, GENOME SEQUENCE, TRANSLOCATED PROTEIN, CHLAMYDIA-TRACHOMATIS, chlamydia, type III secretion, Chlamydophila psittaci

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Chicago
Beeckman, Delphine Sylvie Anne, Tom Geens, Jean-Pierre Timmermans, Patric Van Oostveldt, and Daisy Vanrompay. 2008. “Identification and Characterization of a Type III Secretion System in Chlamydophila Psittaci.” Veterinary Research 39 (3).
APA
Beeckman, D. S. A., Geens, T., Timmermans, J.-P., Van Oostveldt, P., & Vanrompay, D. (2008). Identification and characterization of a type III secretion system in Chlamydophila psittaci. VETERINARY RESEARCH, 39(3).
Vancouver
1.
Beeckman DSA, Geens T, Timmermans J-P, Van Oostveldt P, Vanrompay D. Identification and characterization of a type III secretion system in Chlamydophila psittaci. VETERINARY RESEARCH. 2008;39(3).
MLA
Beeckman, Delphine Sylvie Anne, Tom Geens, Jean-Pierre Timmermans, et al. “Identification and Characterization of a Type III Secretion System in Chlamydophila Psittaci.” VETERINARY RESEARCH 39.3 (2008): n. pag. Print.
@article{419217,
  abstract     = {Chlamydiaceae are obligate intracellular Gram-negative bacteria replicating in vacuoles inside eukaryotic cells. It has been proven that most of them possess a type III secretion system (T3SS) allowing them to transfer effector molecules in the host cell. We examined the existence of a T3SS in Chlamydophila psittaci by studying the expression of three essential structural proteins SctW, SctC, and SctN, and one putative effector protein IncA. Immunofluorescence assays showed SctW and IncA to be associated with the bacteria and the inclusion membrane, while SctC and SctN were only localized to the bacteria itself. Immuno electron microscopy could confirm these results for SctW, IncA, and SctC. Unfortunately, SctN was not investigated with this technique. Additionally, we sequenced 14 full-length T3S genes (scc1, sctW, sctJ, sctL, sctR, sctS, scc2, copD1, sctN, sctQ, sctC, incA, ca037, and cadd) and examined the transcription of 26 Cp. psittaci T3S genes namely cluster 1 (scc1, sctW, sctV, sctU), cluster 2 (sctJ, sctL, sctR, sctS, sctT, scc2, copB1, copD1), cluster 3 (sctD, sctN, ca037, sctQ, pkn5, sctC) and non-clustered genes (incA, incC, scc3, copD2, cap1, tarp, ca530, cadd). The gene expression study indicated the T3S structural protein encoding genes to be transcribed from mid-cycle (12-18 h post infection (p.i.)) on. Genes encoding effector proteins and putative T3S related proteins were expressed early (1.5 h-8 h p.i.) or late (> 24 h p.i.) during the developmental cycle. We hereby provided evidence for the existence of a T3SS and possible effectors in avian Cp. psittaci.},
  articleno    = {27},
  author       = {Beeckman, Delphine Sylvie Anne and Geens, Tom and Timmermans, Jean-Pierre and Van Oostveldt, Patric and Vanrompay, Daisy},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {RECRUITMENT,PHOSPHORYLATION,INCLUSION MEMBRANE,PNEUMONIAE,EXPRESSION,CELLS,AVIAN CHLAMYDIOSIS,GENOME SEQUENCE,TRANSLOCATED PROTEIN,CHLAMYDIA-TRACHOMATIS,chlamydia,type III secretion,Chlamydophila psittaci},
  language     = {eng},
  number       = {3},
  pages        = {18},
  title        = {Identification and characterization of a type III secretion system in Chlamydophila psittaci},
  url          = {http://dx.doi.org/10.1051/vetres:2008002},
  volume       = {39},
  year         = {2008},
}

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