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A membrane-bound NAC transcription factor, ANAC017, mediates mitochondrial retrograde signaling in Arabidopsis

(2013) PLANT CELL. 25(9). p.3450-3471
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Abstract
Plants require daily coordinated regulation of energy metabolism for optimal growth and survival and therefore need to integrate cellular responses with both mitochondrial and plastid retrograde signaling. Using a forward genetic screen to characterize regulators of alternative oxidase1a (rao) mutants, we identified RAO2/Arabidopsis NAC domain-containing protein17 (ANAC017) as a direct positive regulator of AOX1a. RAO2/ANAC017 is targeted to connections and junctions in the endoplasmic reticulum (ER) and F-actin via a C-terminal transmembrane (TM) domain. A consensus rhomboid protease cleavage site is present in ANAC017 just prior to the predicted TM domain. Furthermore, addition of the rhomboid protease inhibitor N-p-Tosyl-L-Phe chloromethyl abolishes the induction of AOX1a upon antimycin A treatment. Simultaneous fluorescent tagging of ANAC017 with N-terminal red fluorescent protein (RFP) and C-terminal green fluorescent protein (GFP) revealed that the N-terminal RFP domain migrated into the nucleus, while the C-terminal GFP tag remained in the ER. Genome-wide analysis of the transcriptional network regulated by RAO2/ANAC017 under stress treatment revealed that RAO2/ANAC017 function was necessary for > 85% of the changes observed as a primary response to cytosolic hydrogen peroxide (H2O2), but only; 33% of transcriptional changes observed in response to antimycin A treatment. Plants with mutated rao2/anac017 were more stress sensitive, whereas a gain-of-function mutation resulted in plants that had lower cellular levels of H2O2 under untreated conditions.
Keywords
PLANT-MITOCHONDRIA, MESSENGER-RNA, DROUGHT STRESS, SINGLET OXYGEN, F-ACTIN, RETICULUM STRESS-RESPONSE, ENDOPLASMIC-RETICULUM, GENE-EXPRESSION, ALTERNATIVE OXIDASE, HIGH LIGHT

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MLA
Ng, Sophia, Aneta Ivanova, Owen Duncan, et al. “A Membrane-bound NAC Transcription Factor, ANAC017, Mediates Mitochondrial Retrograde Signaling in Arabidopsis.” PLANT CELL 25.9 (2013): 3450–3471. Print.
APA
Ng, Sophia, Ivanova, A., Duncan, O., Law, S. R., Van Aken, O., De Clercq, I., Wang, Y., et al. (2013). A membrane-bound NAC transcription factor, ANAC017, mediates mitochondrial retrograde signaling in Arabidopsis. PLANT CELL, 25(9), 3450–3471.
Chicago author-date
Ng, Sophia, Aneta Ivanova, Owen Duncan, Simon R Law, Olivier Van Aken, Inge De Clercq, Yan Wang, et al. 2013. “A Membrane-bound NAC Transcription Factor, ANAC017, Mediates Mitochondrial Retrograde Signaling in Arabidopsis.” Plant Cell 25 (9): 3450–3471.
Chicago author-date (all authors)
Ng, Sophia, Aneta Ivanova, Owen Duncan, Simon R Law, Olivier Van Aken, Inge De Clercq, Yan Wang, Chris Carrie, Lin Xu, Beata Kmiec, Hayden Walker, Frank Van Breusegem, James Whelan, and Estelle Giraud. 2013. “A Membrane-bound NAC Transcription Factor, ANAC017, Mediates Mitochondrial Retrograde Signaling in Arabidopsis.” Plant Cell 25 (9): 3450–3471.
Vancouver
1.
Ng S, Ivanova A, Duncan O, Law SR, Van Aken O, De Clercq I, et al. A membrane-bound NAC transcription factor, ANAC017, mediates mitochondrial retrograde signaling in Arabidopsis. PLANT CELL. 2013;25(9):3450–71.
IEEE
[1]
S. Ng et al., “A membrane-bound NAC transcription factor, ANAC017, mediates mitochondrial retrograde signaling in Arabidopsis,” PLANT CELL, vol. 25, no. 9, pp. 3450–3471, 2013.
@article{4190201,
  abstract     = {{Plants require daily coordinated regulation of energy metabolism for optimal growth and survival and therefore need to integrate cellular responses with both mitochondrial and plastid retrograde signaling. Using a forward genetic screen to characterize regulators of alternative oxidase1a (rao) mutants, we identified RAO2/Arabidopsis NAC domain-containing protein17 (ANAC017) as a direct positive regulator of AOX1a. RAO2/ANAC017 is targeted to connections and junctions in the endoplasmic reticulum (ER) and F-actin via a C-terminal transmembrane (TM) domain. A consensus rhomboid protease cleavage site is present in ANAC017 just prior to the predicted TM domain. Furthermore, addition of the rhomboid protease inhibitor N-p-Tosyl-L-Phe chloromethyl abolishes the induction of AOX1a upon antimycin A treatment. Simultaneous fluorescent tagging of ANAC017 with N-terminal red fluorescent protein (RFP) and C-terminal green fluorescent protein (GFP) revealed that the N-terminal RFP domain migrated into the nucleus, while the C-terminal GFP tag remained in the ER. Genome-wide analysis of the transcriptional network regulated by RAO2/ANAC017 under stress treatment revealed that RAO2/ANAC017 function was necessary for > 85% of the changes observed as a primary response to cytosolic hydrogen peroxide (H2O2), but only; 33% of transcriptional changes observed in response to antimycin A treatment. Plants with mutated rao2/anac017 were more stress sensitive, whereas a gain-of-function mutation resulted in plants that had lower cellular levels of H2O2 under untreated conditions.}},
  author       = {{Ng, Sophia and Ivanova, Aneta and Duncan, Owen and Law, Simon R and Van Aken, Olivier and De Clercq, Inge and Wang, Yan and Carrie, Chris and Xu, Lin and Kmiec, Beata and Walker, Hayden and Van Breusegem, Frank and Whelan, James and Giraud, Estelle}},
  issn         = {{1040-4651}},
  journal      = {{PLANT CELL}},
  keywords     = {{PLANT-MITOCHONDRIA,MESSENGER-RNA,DROUGHT STRESS,SINGLET OXYGEN,F-ACTIN,RETICULUM STRESS-RESPONSE,ENDOPLASMIC-RETICULUM,GENE-EXPRESSION,ALTERNATIVE OXIDASE,HIGH LIGHT}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{3450--3471}},
  title        = {{A membrane-bound NAC transcription factor, ANAC017, mediates mitochondrial retrograde signaling in Arabidopsis}},
  url          = {{http://dx.doi.org/10.1105/tpc.113.113985}},
  volume       = {{25}},
  year         = {{2013}},
}

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