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Bioengineering of plant (tri)terpenoids: from metabolic engineering of plants to synthetic biology in vivo and in vitro

Tessa Moses, Jacob Pollier UGent, Johan M Thevelein and Alain Goossens UGent (2013) NEW PHYTOLOGIST. 200(1). p.27-43
abstract
Terpenoids constitute a large and diverse class of natural products that serve many functions in nature. Most of the tens of thousands of the discovered terpenoids are synthesized by plants, where they function as primary metabolites involved in growth and development, or as secondary metabolites that optimize the interaction between the plant and its environment. Several plant terpenoids are economically important molecules that serve many applications as pharmaceuticals, pesticides, etc. Major challenges for the commercialization of plant-derived terpenoids include their low production levels inplanta and the continuous demand of industry for novel molecules with new or superior biological activities. Here, we highlight several synthetic biology methods to enhance and diversify the production of plant terpenoids, with a foresight towards triterpenoid engineering, the least engineered class of bioactive terpenoids. Increased or cheaper production of valuable triterpenoids may be obtained by classic' metabolic engineering of plants or by heterologous production of the compounds in other plants or microbes. Novel triterpenoid structures can be generated through combinatorial biosynthesis or directed enzyme evolution approaches. In its ultimate form, synthetic biology may lead to the production of large amounts of plant triterpenoids in invitro systems or custom-designed artificial biological systems.
Please use this url to cite or link to this publication:
author
organization
alternative title
Bioengineering of plant (tri)terpenoids : from metabolic engineering of plants to synthetic biology invivo and invitro
year
type
journalArticle (review)
publication status
published
subject
keyword
synthetic biology, terpenoids, secondary metabolism, heterologous biosynthesis, directed enzyme evolution, combinatorial biosynthesis, bioengineering, triterpenoids, ADVANCED SOLID TUMORS, COENZYME-A REDUCTASE, ESCHERICHIA-COLI, SACCHAROMYCES-CEREVISIAE, DIRECTED EVOLUTION, COMBINATORIAL BIOSYNTHESIS, SECONDARY METABOLISM, ISOPRENOID PATHWAY, NATURAL-PRODUCTS, PHASE-I
journal title
NEW PHYTOLOGIST
New Phytol.
volume
200
issue
1
pages
27 - 43
Web of Science type
Review
Web of Science id
000323475000007
JCR category
PLANT SCIENCES
JCR impact factor
6.545 (2013)
JCR rank
9/199 (2013)
JCR quartile
1 (2013)
ISSN
1469-8137
DOI
10.1111/nph.12325
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4190192
handle
http://hdl.handle.net/1854/LU-4190192
date created
2013-11-26 15:54:25
date last changed
2016-12-19 15:38:06
@article{4190192,
  abstract     = {Terpenoids constitute a large and diverse class of natural products that serve many functions in nature. Most of the tens of thousands of the discovered terpenoids are synthesized by plants, where they function as primary metabolites involved in growth and development, or as secondary metabolites that optimize the interaction between the plant and its environment. Several plant terpenoids are economically important molecules that serve many applications as pharmaceuticals, pesticides, etc. Major challenges for the commercialization of plant-derived terpenoids include their low production levels inplanta and the continuous demand of industry for novel molecules with new or superior biological activities. Here, we highlight several synthetic biology methods to enhance and diversify the production of plant terpenoids, with a foresight towards triterpenoid engineering, the least engineered class of bioactive terpenoids. Increased or cheaper production of valuable triterpenoids may be obtained by classic' metabolic engineering of plants or by heterologous production of the compounds in other plants or microbes. Novel triterpenoid structures can be generated through combinatorial biosynthesis or directed enzyme evolution approaches. In its ultimate form, synthetic biology may lead to the production of large amounts of plant triterpenoids in invitro systems or custom-designed artificial biological systems.},
  author       = {Moses, Tessa and Pollier, Jacob and Thevelein, Johan M and Goossens, Alain},
  issn         = {1469-8137},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {synthetic biology,terpenoids,secondary metabolism,heterologous biosynthesis,directed enzyme evolution,combinatorial biosynthesis,bioengineering,triterpenoids,ADVANCED SOLID TUMORS,COENZYME-A REDUCTASE,ESCHERICHIA-COLI,SACCHAROMYCES-CEREVISIAE,DIRECTED EVOLUTION,COMBINATORIAL BIOSYNTHESIS,SECONDARY METABOLISM,ISOPRENOID PATHWAY,NATURAL-PRODUCTS,PHASE-I},
  language     = {eng},
  number       = {1},
  pages        = {27--43},
  title        = {Bioengineering of plant (tri)terpenoids: from metabolic engineering of plants to synthetic biology in vivo and in vitro},
  url          = {http://dx.doi.org/10.1111/nph.12325},
  volume       = {200},
  year         = {2013},
}

Chicago
Moses, Tessa, Jacob Pollier, Johan M Thevelein, and Alain Goossens. 2013. “Bioengineering of Plant (tri)terpenoids: From Metabolic Engineering of Plants to Synthetic Biology in Vivo and in Vitro.” New Phytologist 200 (1): 27–43.
APA
Moses, T., Pollier, J., Thevelein, J. M., & Goossens, A. (2013). Bioengineering of plant (tri)terpenoids: from metabolic engineering of plants to synthetic biology in vivo and in vitro. NEW PHYTOLOGIST, 200(1), 27–43.
Vancouver
1.
Moses T, Pollier J, Thevelein JM, Goossens A. Bioengineering of plant (tri)terpenoids: from metabolic engineering of plants to synthetic biology in vivo and in vitro. NEW PHYTOLOGIST. 2013;200(1):27–43.
MLA
Moses, Tessa, Jacob Pollier, Johan M Thevelein, et al. “Bioengineering of Plant (tri)terpenoids: From Metabolic Engineering of Plants to Synthetic Biology in Vivo and in Vitro.” NEW PHYTOLOGIST 200.1 (2013): 27–43. Print.