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SILAC-based proteome analysis of Starmerella bombicola sophorolipid production

Katarzyna Ciesielska (UGent) , Bing Li (UGent) , Sara Groeneboer (UGent) , Inge Van Bogaert (UGent) , Yao-Cheng Lin (UGent) , Wim Soetaert (UGent) , Yves Van de Peer (UGent) and Bart Devreese (UGent)
(2013) JOURNAL OF PROTEOME RESEARCH. 12(10). p.4376-4392
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Abstract
Starmerella (Candida) bombicola is the biosurfactant-producing species that caught the greatest deal of attention in the academic and industrial world due to its ability of producing large amounts of sophorolipids. Despite its high economic potential, the biochemistry behind the sophorolipid biosynthesis is still poorly understood. Here we present the first proteomic characterization of S. bombicola for which we created a lys1 Delta. mutant to allow the use of SILAC for quantitative analysis. To characterize the processes behind the production of these biosurfactants, we compared the proteome of sophorolipid producing (early stationary phase) and nonproducing cells (exponential phase). We report the simultaneous production of all known enzymes involved in sophorolipid biosynthesis including a predicted sophorolipid transporter. In addition, we identified the heme binding protein Dap1 as a possible regulator for Cyp52M1. Our results further indicate that ammonium and phosphate limitation are not the sole limiting factors inducing sophorolipid biosynthesis.
Keywords
BIOSYNTHESIS GENE-CLUSTER, YEAST SACCHAROMYCES CEREVISIAE, mRNA-seq, SILAC, quantitative proteomics, yeast, sophorolipid biosynthesis, Starmerella bombicola, HEAT SHOCK PROTEIN, CANDIDA BOMBICOLA, MICROBIAL PRODUCTION, CYTOCHROME P450 ENZYMES, ACTIN CYTOSKELETON, MASS SPECTROMETRY, OXIDATIVE STRESS, BINDING PROTEIN

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Citation

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MLA
Ciesielska, Katarzyna, et al. “SILAC-Based Proteome Analysis of Starmerella Bombicola Sophorolipid Production.” JOURNAL OF PROTEOME RESEARCH, vol. 12, no. 10, 2013, pp. 4376–92, doi:10.1021/pr400392a.
APA
Ciesielska, K., Li, B., Groeneboer, S., Van Bogaert, I., Lin, Y.-C., Soetaert, W., … Devreese, B. (2013). SILAC-based proteome analysis of Starmerella bombicola sophorolipid production. JOURNAL OF PROTEOME RESEARCH, 12(10), 4376–4392. https://doi.org/10.1021/pr400392a
Chicago author-date
Ciesielska, Katarzyna, Bing Li, Sara Groeneboer, Inge Van Bogaert, Yao-Cheng Lin, Wim Soetaert, Yves Van de Peer, and Bart Devreese. 2013. “SILAC-Based Proteome Analysis of Starmerella Bombicola Sophorolipid Production.” JOURNAL OF PROTEOME RESEARCH 12 (10): 4376–92. https://doi.org/10.1021/pr400392a.
Chicago author-date (all authors)
Ciesielska, Katarzyna, Bing Li, Sara Groeneboer, Inge Van Bogaert, Yao-Cheng Lin, Wim Soetaert, Yves Van de Peer, and Bart Devreese. 2013. “SILAC-Based Proteome Analysis of Starmerella Bombicola Sophorolipid Production.” JOURNAL OF PROTEOME RESEARCH 12 (10): 4376–4392. doi:10.1021/pr400392a.
Vancouver
1.
Ciesielska K, Li B, Groeneboer S, Van Bogaert I, Lin Y-C, Soetaert W, et al. SILAC-based proteome analysis of Starmerella bombicola sophorolipid production. JOURNAL OF PROTEOME RESEARCH. 2013;12(10):4376–92.
IEEE
[1]
K. Ciesielska et al., “SILAC-based proteome analysis of Starmerella bombicola sophorolipid production,” JOURNAL OF PROTEOME RESEARCH, vol. 12, no. 10, pp. 4376–4392, 2013.
@article{4187598,
  abstract     = {{Starmerella (Candida) bombicola is the biosurfactant-producing species that caught the greatest deal of attention in the academic and industrial world due to its ability of producing large amounts of sophorolipids. Despite its high economic potential, the biochemistry behind the sophorolipid biosynthesis is still poorly understood. Here we present the first proteomic characterization of S. bombicola for which we created a lys1 Delta. mutant to allow the use of SILAC for quantitative analysis. To characterize the processes behind the production of these biosurfactants, we compared the proteome of sophorolipid producing (early stationary phase) and nonproducing cells (exponential phase). We report the simultaneous production of all known enzymes involved in sophorolipid biosynthesis including a predicted sophorolipid transporter. In addition, we identified the heme binding protein Dap1 as a possible regulator for Cyp52M1. Our results further indicate that ammonium and phosphate limitation are not the sole limiting factors inducing sophorolipid biosynthesis.}},
  author       = {{Ciesielska, Katarzyna and Li, Bing and Groeneboer, Sara and Van Bogaert, Inge and Lin, Yao-Cheng and Soetaert, Wim and Van de Peer, Yves and Devreese, Bart}},
  issn         = {{1535-3893}},
  journal      = {{JOURNAL OF PROTEOME RESEARCH}},
  keywords     = {{BIOSYNTHESIS GENE-CLUSTER,YEAST SACCHAROMYCES CEREVISIAE,mRNA-seq,SILAC,quantitative proteomics,yeast,sophorolipid biosynthesis,Starmerella bombicola,HEAT SHOCK PROTEIN,CANDIDA BOMBICOLA,MICROBIAL PRODUCTION,CYTOCHROME P450 ENZYMES,ACTIN CYTOSKELETON,MASS SPECTROMETRY,OXIDATIVE STRESS,BINDING PROTEIN}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{4376--4392}},
  title        = {{SILAC-based proteome analysis of Starmerella bombicola sophorolipid production}},
  url          = {{http://doi.org/10.1021/pr400392a}},
  volume       = {{12}},
  year         = {{2013}},
}
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