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Plant protein interactomes

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Abstract
Protein-protein interactions are a critical element of biological systems, and the analysis of interaction partners can provide valuable hints about unknown functions of a protein. In recent years, several large-scale protein interaction studies have begun to unravel the complex networks through which plant proteins exert their functions. Two major classes of experimental approaches are used for protein interaction mapping: analysis of direct interactions using binary methods such as yeast two-hybrid or split ubiquitin, and analysis of protein complexes through affinity purification followed by mass spectrometry. In addition, bioinformatics predictions can suggest interactions that have evaded detection by other methods or those of proteins that have not been investigated. Here we review the major approaches to construct, analyze, use, and carry out quality control on plant protein interactome networks. We present experimental and computational approaches for large-scale mapping, methods for validation or smaller-scale functional studies, important bioinformatics resources, and findings from recently published large-scale plant interactome network maps.
Keywords
MOLECULAR INTERACTION DATABASE, YEAST 2-HYBRID SYSTEM, TANDEM AFFINITY PURIFICATION, BIMOLECULAR FLUORESCENCE COMPLEMENTATION, model plants and crops, networks, functional genomics, protein-protein interactions, TRANSCRIPTION FACTOR COMPLEXES, INTERACTIONS IN PLANTA, GENE EXPRESSION DATA, ARABIDOPSIS THALIANA, INTERACTION NETWORK, INTERACTION MAP

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Citation

Please use this url to cite or link to this publication:

MLA
Braun, Pascal, Sébastien Aubourg, Jelle Van Leene, et al. “Plant Protein Interactomes.” Annual Review of Plant Biology 64 (2013): 161–187. Print.
APA
Braun, P., Aubourg, S., Van Leene, J., De Jaeger, G., & Lurin, C. (2013). Plant protein interactomes. Annual Review of Plant Biology, 64, 161–187.
Chicago author-date
Braun, Pascal, Sébastien Aubourg, Jelle Van Leene, Geert De Jaeger, and Claire Lurin. 2013. “Plant Protein Interactomes.” Annual Review of Plant Biology 64: 161–187.
Chicago author-date (all authors)
Braun, Pascal, Sébastien Aubourg, Jelle Van Leene, Geert De Jaeger, and Claire Lurin. 2013. “Plant Protein Interactomes.” Annual Review of Plant Biology 64: 161–187.
Vancouver
1.
Braun P, Aubourg S, Van Leene J, De Jaeger G, Lurin C. Plant protein interactomes. Annual Review of Plant Biology. Palo Alto, CA, USA: Annual Reviews; 2013;64:161–87.
IEEE
[1]
P. Braun, S. Aubourg, J. Van Leene, G. De Jaeger, and C. Lurin, “Plant protein interactomes,” Annual Review of Plant Biology, vol. 64, pp. 161–187, 2013.
@article{4187559,
  abstract     = {Protein-protein interactions are a critical element of biological systems, and the analysis of interaction partners can provide valuable hints about unknown functions of a protein. In recent years, several large-scale protein interaction studies have begun to unravel the complex networks through which plant proteins exert their functions. Two major classes of experimental approaches are used for protein interaction mapping: analysis of direct interactions using binary methods such as yeast two-hybrid or split ubiquitin, and analysis of protein complexes through affinity purification followed by mass spectrometry. In addition, bioinformatics predictions can suggest interactions that have evaded detection by other methods or those of proteins that have not been investigated. Here we review the major approaches to construct, analyze, use, and carry out quality control on plant protein interactome networks. We present experimental and computational approaches for large-scale mapping, methods for validation or smaller-scale functional studies, important bioinformatics resources, and findings from recently published large-scale plant interactome network maps.},
  author       = {Braun, Pascal and Aubourg, Sébastien and Van Leene, Jelle and De Jaeger, Geert and Lurin, Claire},
  isbn         = {9780824306649},
  issn         = {1543-5008},
  journal      = {Annual Review of Plant Biology},
  keywords     = {MOLECULAR INTERACTION DATABASE,YEAST 2-HYBRID SYSTEM,TANDEM AFFINITY PURIFICATION,BIMOLECULAR FLUORESCENCE COMPLEMENTATION,model plants and crops,networks,functional genomics,protein-protein interactions,TRANSCRIPTION FACTOR COMPLEXES,INTERACTIONS IN PLANTA,GENE EXPRESSION DATA,ARABIDOPSIS THALIANA,INTERACTION NETWORK,INTERACTION MAP},
  language     = {eng},
  pages        = {161--187},
  publisher    = {Annual Reviews},
  title        = {Plant protein interactomes},
  url          = {http://dx.doi.org/10.1146/annurev-arplant-050312-120140},
  volume       = {64},
  year         = {2013},
}

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