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Immature seed rescue and abscisic acid quantification in Rosa hybrida L. suggest early and transient endodormancy

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Abstract
Breeding is a long process in the rose industry. Premature death of developing embryos due to incompatibility and delayed germination, caused by seed dormancy, are the main causes. Immature seed rescue may offer a valuable way to increase hybridisation efficiency, to introduce genotypes with interesting ornamental traits, and to shorten time needed to obtain seedlings. In this study, in vitro immature seed rescue was carried out in Rosa hybrida L. Seeds from 7 to 28 days after pollination (DAP) were sterilized and placed onto two germination media: Murashige and Skoog medium (full strength macro and micro nutrients and vitamins, MS0) and MS0 with 6-benzylaminopurine (BAP; 2.5 mg/L) and gibberellic acid 3 (GA3; 0.5 mg/L). Four different conditions of light and temperature were applied. At 15 and 30 DAP, the abscisic acid (ABA) concentration was quantified in fully formed embryos of two different genotypes of hybrid roses. Preliminary data showed that only seeds harvested at 21 DAP germinated in both media, this after 2 months of in vitro culture. This result is supported by the observation that basal levels of ABA were found in embryos at 30 DAP. The general role of ABA on preventing precocious germination and vivipary suggests an early and transient endodormancy in embryos of hybrid roses. The integration of these two approaches with the aim of defining protocols for precocious in vitro germination and investigating the pathway of dormancy in R. hybrida is highlighted.
Keywords
in vitro culture, immature seed, breeding, plant growth regulators, ABA, GERMINATION, DORMANCY

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Citation

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Chicago
Caser, Matteo, Luca Pipino, Marie-Christine Van Labeke, Andrea Mansuino, Annelisa Giovannini, and Valentina Scariot. 2012. “Immature Seed Rescue and Abscisic Acid Quantification in Rosa Hybrida L. Suggest Early and Transient Endodormancy.” In Acta Horticulturae, ed. Danny Geelen, 961:593–598. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Caser, M., Pipino, L., Van Labeke, M.-C., Mansuino, A., Giovannini, A., & Scariot, V. (2012). Immature seed rescue and abscisic acid quantification in Rosa hybrida L. suggest early and transient endodormancy. In D. Geelen (Ed.), Acta Horticulturae (Vol. 961, pp. 593–598). Presented at the 7th International symposium on In Vitro Culture and Horticultural Breeding, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Caser M, Pipino L, Van Labeke M-C, Mansuino A, Giovannini A, Scariot V. Immature seed rescue and abscisic acid quantification in Rosa hybrida L. suggest early and transient endodormancy. In: Geelen D, editor. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2012. p. 593–8.
MLA
Caser, Matteo, Luca Pipino, Marie-Christine Van Labeke, et al. “Immature Seed Rescue and Abscisic Acid Quantification in Rosa Hybrida L. Suggest Early and Transient Endodormancy.” Acta Horticulturae. Ed. Danny Geelen. Vol. 961. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2012. 593–598. Print.
@inproceedings{4185153,
  abstract     = {Breeding is a long process in the rose industry. Premature death of developing embryos due to incompatibility and delayed germination, caused by seed dormancy, are the main causes. Immature seed rescue may offer a valuable way to increase hybridisation efficiency, to introduce genotypes with interesting ornamental traits, and to shorten time needed to obtain seedlings. In this study, in vitro immature seed rescue was carried out in Rosa hybrida L. Seeds from 7 to 28 days after pollination (DAP) were sterilized and placed onto two germination media: Murashige and Skoog medium (full strength macro and micro nutrients and vitamins, MS0) and MS0 with 6-benzylaminopurine (BAP; 2.5 mg/L) and gibberellic acid 3 (GA3; 0.5 mg/L). Four different conditions of light and temperature were applied. At 15 and 30 DAP, the abscisic acid (ABA) concentration was quantified in fully formed embryos of two different genotypes of hybrid roses. Preliminary data showed that only seeds harvested at 21 DAP germinated in both media, this after 2 months of in vitro culture. This result is supported by the observation that basal levels of ABA were found in embryos at 30 DAP. The general role of ABA on preventing precocious germination and vivipary suggests an early and transient endodormancy in embryos of hybrid roses. The integration of these two approaches with the aim of defining protocols for precocious in vitro germination and investigating the pathway of dormancy in R. hybrida is highlighted.},
  author       = {Caser, Matteo and Pipino, Luca and Van Labeke, Marie-Christine and Mansuino, Andrea and Giovannini, Annelisa and Scariot, Valentina},
  booktitle    = {Acta Horticulturae},
  editor       = {Geelen, Danny},
  isbn         = {9789066055957},
  issn         = {0567-7572},
  keywords     = {in vitro culture,immature seed,breeding,plant growth regulators,ABA,GERMINATION,DORMANCY},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {593--598},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Immature seed rescue and abscisic acid quantification in Rosa hybrida L. suggest early and transient endodormancy},
  url          = {http://www.actahort.org/books/961/961_75.htm},
  volume       = {961},
  year         = {2012},
}

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