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Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species

Hilde Moyaert (UGent) , Frank Pasmans (UGent) , Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) and Margo Baele (UGent)
(2008) JOURNAL OF CLINICAL MICROBIOLOGY. 46(5). p.1867-1869
Author
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Abstract
The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
Keywords
CAL SAMPLES, CAMPYLOBACTER, FELIS

Citation

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Chicago
Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, and Margo Baele. 2008. “Evaluation of 16S rRNA Gene-based PCR Assays for Genus-level Identification of Helicobacter Species.” Journal of Clinical Microbiology 46 (5): 1867–1869.
APA
Moyaert, H., Pasmans, F., Ducatelle, R., Haesebrouck, F., & Baele, M. (2008). Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY, 46(5), 1867–1869.
Vancouver
1.
Moyaert H, Pasmans F, Ducatelle R, Haesebrouck F, Baele M. Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY. 2008;46(5):1867–9.
MLA
Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, et al. “Evaluation of 16S rRNA Gene-based PCR Assays for Genus-level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY 46.5 (2008): 1867–1869. Print.
@article{418177,
  abstract     = {The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100\% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5\%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.},
  author       = {Moyaert, Hilde and Pasmans, Frank and Ducatelle, Richard and Haesebrouck, Freddy and Baele, Margo},
  issn         = {0095-1137},
  journal      = {JOURNAL OF CLINICAL MICROBIOLOGY},
  keyword      = {CAL SAMPLES,CAMPYLOBACTER,FELIS},
  language     = {eng},
  number       = {5},
  pages        = {1867--1869},
  title        = {Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species},
  url          = {http://dx.doi.org/10.1128/JCM.00139-08},
  volume       = {46},
  year         = {2008},
}

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