Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species
- Author
- Hilde Moyaert (UGent) , Frank Pasmans (UGent) , Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) and Margo Baele (UGent)
- Organization
- Abstract
- The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
- Keywords
- CAL SAMPLES, CAMPYLOBACTER, FELIS
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-418177
- Chicago
- Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, and Margo Baele. 2008. “Evaluation of 16S rRNA Gene-based PCR Assays for Genus-level Identification of Helicobacter Species.” Journal of Clinical Microbiology 46 (5): 1867–1869.
- APA
- Moyaert, H., Pasmans, F., Ducatelle, R., Haesebrouck, F., & Baele, M. (2008). Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY, 46(5), 1867–1869.
- Vancouver
- 1.Moyaert H, Pasmans F, Ducatelle R, Haesebrouck F, Baele M. Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY. 2008;46(5):1867–9.
- MLA
- Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, et al. “Evaluation of 16S rRNA Gene-based PCR Assays for Genus-level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY 46.5 (2008): 1867–1869. Print.
@article{418177,
abstract = {The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100\% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5\%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.},
author = {Moyaert, Hilde and Pasmans, Frank and Ducatelle, Richard and Haesebrouck, Freddy and Baele, Margo},
issn = {0095-1137},
journal = {JOURNAL OF CLINICAL MICROBIOLOGY},
keyword = {CAL SAMPLES,CAMPYLOBACTER,FELIS},
language = {eng},
number = {5},
pages = {1867--1869},
title = {Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species},
url = {http://dx.doi.org/10.1128/JCM.00139-08},
volume = {46},
year = {2008},
}
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