
Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species
- Author
- Hilde Moyaert (UGent) , Frank Pasmans (UGent) , Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) and Margo Baele (UGent)
- Organization
- Abstract
- The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
- Keywords
- CAL SAMPLES, CAMPYLOBACTER, FELIS
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-418177
- MLA
- Moyaert, Hilde, et al. “Evaluation of 16S RRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY, vol. 46, no. 5, 2008, pp. 1867–69, doi:10.1128/JCM.00139-08.
- APA
- Moyaert, H., Pasmans, F., Ducatelle, R., Haesebrouck, F., & Baele, M. (2008). Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY, 46(5), 1867–1869. https://doi.org/10.1128/JCM.00139-08
- Chicago author-date
- Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, and Margo Baele. 2008. “Evaluation of 16S RRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY 46 (5): 1867–69. https://doi.org/10.1128/JCM.00139-08.
- Chicago author-date (all authors)
- Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, and Margo Baele. 2008. “Evaluation of 16S RRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY 46 (5): 1867–1869. doi:10.1128/JCM.00139-08.
- Vancouver
- 1.Moyaert H, Pasmans F, Ducatelle R, Haesebrouck F, Baele M. Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY. 2008;46(5):1867–9.
- IEEE
- [1]H. Moyaert, F. Pasmans, R. Ducatelle, F. Haesebrouck, and M. Baele, “Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species,” JOURNAL OF CLINICAL MICROBIOLOGY, vol. 46, no. 5, pp. 1867–1869, 2008.
@article{418177, abstract = {{The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.}}, author = {{Moyaert, Hilde and Pasmans, Frank and Ducatelle, Richard and Haesebrouck, Freddy and Baele, Margo}}, issn = {{0095-1137}}, journal = {{JOURNAL OF CLINICAL MICROBIOLOGY}}, keywords = {{CAL SAMPLES,CAMPYLOBACTER,FELIS}}, language = {{eng}}, number = {{5}}, pages = {{1867--1869}}, title = {{Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species}}, url = {{http://dx.doi.org/10.1128/JCM.00139-08}}, volume = {{46}}, year = {{2008}}, }
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