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Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species

Hilde Moyaert (UGent) , Frank Pasmans (UGent) , Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) and Margo Baele (UGent)
(2008) JOURNAL OF CLINICAL MICROBIOLOGY. 46(5). p.1867-1869
Author
Organization
Abstract
The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
Keywords
CAL SAMPLES, CAMPYLOBACTER, FELIS

Citation

Please use this url to cite or link to this publication:

MLA
Moyaert, Hilde, et al. “Evaluation of 16S RRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY, vol. 46, no. 5, 2008, pp. 1867–69, doi:10.1128/JCM.00139-08.
APA
Moyaert, H., Pasmans, F., Ducatelle, R., Haesebrouck, F., & Baele, M. (2008). Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY, 46(5), 1867–1869. https://doi.org/10.1128/JCM.00139-08
Chicago author-date
Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, and Margo Baele. 2008. “Evaluation of 16S RRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY 46 (5): 1867–69. https://doi.org/10.1128/JCM.00139-08.
Chicago author-date (all authors)
Moyaert, Hilde, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, and Margo Baele. 2008. “Evaluation of 16S RRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species.” JOURNAL OF CLINICAL MICROBIOLOGY 46 (5): 1867–1869. doi:10.1128/JCM.00139-08.
Vancouver
1.
Moyaert H, Pasmans F, Ducatelle R, Haesebrouck F, Baele M. Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species. JOURNAL OF CLINICAL MICROBIOLOGY. 2008;46(5):1867–9.
IEEE
[1]
H. Moyaert, F. Pasmans, R. Ducatelle, F. Haesebrouck, and M. Baele, “Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species,” JOURNAL OF CLINICAL MICROBIOLOGY, vol. 46, no. 5, pp. 1867–1869, 2008.
@article{418177,
  abstract     = {{The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.}},
  author       = {{Moyaert, Hilde and Pasmans, Frank and Ducatelle, Richard and Haesebrouck, Freddy and Baele, Margo}},
  issn         = {{0095-1137}},
  journal      = {{JOURNAL OF CLINICAL MICROBIOLOGY}},
  keywords     = {{CAL SAMPLES,CAMPYLOBACTER,FELIS}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1867--1869}},
  title        = {{Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species}},
  url          = {{http://dx.doi.org/10.1128/JCM.00139-08}},
  volume       = {{46}},
  year         = {{2008}},
}
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