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The need for transparency and good practices in the qPCR literature

Stephen A Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory Shipley, et al.
(2013) NATURE METHODS. 10(11). p.1063-1067
Author
Organization
Abstract
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
Keywords
NORMALIZATION, PUBLICATION, QUANTIFICATION, RNA, RT-PCR, CANCER-CELLS, MIQE GUIDELINES, QUANTITATIVE PCR, POLYMERASE CHAIN-REACTION, REAL-TIME PCR

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Citation

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MLA
Bustin, Stephen A., et al. “The Need for Transparency and Good Practices in the QPCR Literature.” NATURE METHODS, vol. 10, no. 11, 2013, pp. 1063–67, doi:10.1038/nmeth.2697.
APA
Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista, M., … Vandesompele, J. (2013). The need for transparency and good practices in the qPCR literature. NATURE METHODS, 10(11), 1063–1067. https://doi.org/10.1038/nmeth.2697
Chicago author-date
Bustin, Stephen A, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, et al. 2013. “The Need for Transparency and Good Practices in the QPCR Literature.” NATURE METHODS 10 (11): 1063–67. https://doi.org/10.1038/nmeth.2697.
Chicago author-date (all authors)
Bustin, Stephen A, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory Shipley, Carl T Wittwer, Peter Schjerling, Philip J Day, Mónica Abreu, Begoña Aguado, Jean-François Beaulieu, Anneleen Beckers, Sarah Bogaert, John A Browne, Fernando Carrasco-Ramiro, Liesbeth Ceelen, Kate Ciborowski, Pieter Cornillie, Stephanie Coulon, Ann Cuypers, Sara De Brouwer, Leentje De Ceuninck, Jurgen De Craene, Hélène De Naeyer, Ward De Spiegelaere, Kato Deckers, Annelies Dheedene, Kaat Durinck, Margarida Ferreira-Teixeira, Annelies Fieuw, Jack M Gallup, Sandra Gonzalo-Flores, Karen Goossens, Femke Heindryckx, Elizabeth Herring, Hans Hoenicka, Laura Icardi, Rolf Jaggi, Farzad Javad, Michail Karampelias, Frederick Kibenge, Molly Kibenge, Candy Kumps, Irina Lambertz, Tim Lammens, Amelia Markey, Peter Messiaen, Evelien Mets, Sofia Morais, Alberto Mudarra-Rubio, Justine Nakiwala, Hilde Nelis, Pal A Olsvik, Claudina Perez Novo, Michelle Plusquin, Tony Remans, Ali Rihani, Paulo Rodrigues-Santos, Pieter Rondou, Rebecca Sanders, Katharina Schmidt-Bleek, Kerstin Skovgaard, Karen Smeets, Laura Tabera, Stefan Toegel, Tim Van Acker, Wim Van Den Broeck, Joni Van der Meulen, Mireille Van Gele, Gert Van Peer, Mario Van Poucke, Nadine Van Roy, Sarah Vergult, Joris Wauman, Marina Tshuikina-Wiklander, Erik Willems, Sara Zaccara, Fjoralba Zeka, and Jo Vandesompele. 2013. “The Need for Transparency and Good Practices in the QPCR Literature.” NATURE METHODS 10 (11): 1063–1067. doi:10.1038/nmeth.2697.
Vancouver
1.
Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, et al. The need for transparency and good practices in the qPCR literature. NATURE METHODS. 2013;10(11):1063–7.
IEEE
[1]
S. A. Bustin et al., “The need for transparency and good practices in the qPCR literature,” NATURE METHODS, vol. 10, no. 11, pp. 1063–1067, 2013.
@article{4181351,
  abstract     = {{Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.}},
  author       = {{Bustin, Stephen A and Benes, Vladimir and Garson, Jeremy and Hellemans, Jan and Huggett, Jim and Kubista, Mikael and Mueller, Reinhold and Nolan, Tania and Pfaffl, Michael W and Shipley, Gregory and Wittwer, Carl T and Schjerling, Peter and Day, Philip J and Abreu, Mónica and Aguado, Begoña and Beaulieu, Jean-François and Beckers, Anneleen and Bogaert, Sarah and Browne, John A and Carrasco-Ramiro, Fernando and Ceelen, Liesbeth and Ciborowski, Kate and Cornillie, Pieter and Coulon, Stephanie and Cuypers, Ann and De Brouwer, Sara and De Ceuninck, Leentje and De Craene, Jurgen and De Naeyer, Hélène and De Spiegelaere, Ward and Deckers, Kato and Dheedene, Annelies and Durinck, Kaat and Ferreira-Teixeira, Margarida and Fieuw, Annelies and Gallup, Jack M and Gonzalo-Flores, Sandra and Goossens, Karen and Heindryckx, Femke and Herring, Elizabeth and Hoenicka, Hans and Icardi, Laura and Jaggi, Rolf and Javad, Farzad and Karampelias, Michail and Kibenge, Frederick and Kibenge, Molly and Kumps, Candy and Lambertz, Irina and Lammens, Tim and Markey, Amelia and Messiaen, Peter and Mets, Evelien and Morais, Sofia and Mudarra-Rubio, Alberto and Nakiwala, Justine and Nelis, Hilde and Olsvik, Pal A and Perez Novo, Claudina and Plusquin, Michelle and Remans, Tony and Rihani, Ali and Rodrigues-Santos, Paulo and Rondou, Pieter and Sanders, Rebecca and Schmidt-Bleek, Katharina and Skovgaard, Kerstin and Smeets, Karen and Tabera, Laura and Toegel, Stefan and Van Acker, Tim and Van Den Broeck, Wim and Van der Meulen, Joni and Van Gele, Mireille and Van Peer, Gert and Van Poucke, Mario and Van Roy, Nadine and Vergult, Sarah and Wauman, Joris and Tshuikina-Wiklander, Marina and Willems, Erik and Zaccara, Sara and Zeka, Fjoralba and Vandesompele, Jo}},
  issn         = {{1548-7091}},
  journal      = {{NATURE METHODS}},
  keywords     = {{NORMALIZATION,PUBLICATION,QUANTIFICATION,RNA,RT-PCR,CANCER-CELLS,MIQE GUIDELINES,QUANTITATIVE PCR,POLYMERASE CHAIN-REACTION,REAL-TIME PCR}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1063--1067}},
  title        = {{The need for transparency and good practices in the qPCR literature}},
  url          = {{http://doi.org/10.1038/nmeth.2697}},
  volume       = {{10}},
  year         = {{2013}},
}
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