Ghent University Academic Bibliography

Advanced

The need for transparency and good practices in the qPCR literature

Stephen A Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory Shipley, et al. (2013) NATURE METHODS. 10(11). p.1063-1067
abstract
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NORMALIZATION, PUBLICATION, QUANTIFICATION, RNA, RT-PCR, CANCER-CELLS, MIQE GUIDELINES, QUANTITATIVE PCR, POLYMERASE CHAIN-REACTION, REAL-TIME PCR
journal title
NATURE METHODS
Nat. Methods
volume
10
issue
11
pages
1063 - 1067
Web of Science type
Article
Web of Science id
000326507600016
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
25.953 (2013)
JCR rank
1/78 (2013)
JCR quartile
1 (2013)
ISSN
1548-7091
DOI
10.1038/nmeth.2697
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4181351
handle
http://hdl.handle.net/1854/LU-4181351
date created
2013-11-08 08:15:09
date last changed
2017-09-01 13:05:04
@article{4181351,
  abstract     = {Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.},
  author       = {Bustin, Stephen A and Benes, Vladimir and Garson, Jeremy and Hellemans, Jan and Huggett, Jim and Kubista, Mikael and Mueller, Reinhold and Nolan, Tania and Pfaffl, Michael W and Shipley, Gregory and Wittwer, Carl T and Schjerling, Peter and Day, Philip J and Abreu, M{\'o}nica and Aguado, Bego{\~n}a and Beaulieu, Jean-Fran\c{c}ois and Beckers, Anneleen and Bogaert, Sarah and Browne, John A and Carrasco-Ramiro, Fernando and Ceelen, Liesbeth and Ciborowski, Kate and Cornillie, Pieter and Coulon, Stephanie and Cuypers, Ann and De Brouwer, Sara and De Ceuninck, Leentje and De Craene, Jurgen and DE NAEYER, HELENE and De Spiegelaere, Ward and Deckers, Kato and DHEEDENE, ANNELIES and Durinck, Kaat and Ferreira-Teixeira, Margarida and Fieuw, Annelies and Gallup, Jack M and Gonzalo-Flores, Sandra and Goossens, Karen and Heindryckx, Femke and Herring, Elizabeth and Hoenicka, Hans and Icardi, Laura and Jaggi, Rolf and Javad, Farzad and Karampelias, Michail and Kibenge, Frederick and Kibenge, Molly and Kumps, Candy and Lambertz, Irina and Lammens, Tim and Markey, Amelia and Messiaen, Peter and Mets, Evelien and Morais, Sofia and Mudarra-Rubio, Alberto and Nakiwala, Justine and Nelis, Hilde and Olsvik, Pal A and Perez Novo, Claudina and Plusquin, Michelle and Remans, Tony and Rihani, Ali and Rodrigues-Santos, Paulo and Rondou, Pieter and Sanders, Rebecca and Schmidt-Bleek, Katharina and Skovgaard, Kerstin and Smeets, Karen and Tabera, Laura and Toegel, Stefan and Van Acker, Tim and Van Den Broeck, Wim and Van der Meulen, Joni and Van Gele, Mireille and Van Peer, Gert and Van Poucke, Mario and Van Roy, Nadine and Vergult, Sarah and Wauman, Joris and Tshuikina-Wiklander, Marina and Willems, Erik and Zaccara, Sara and Zeka, Fjoralba and Vandesompele, Jo},
  issn         = {1548-7091},
  journal      = {NATURE METHODS},
  keyword      = {NORMALIZATION,PUBLICATION,QUANTIFICATION,RNA,RT-PCR,CANCER-CELLS,MIQE GUIDELINES,QUANTITATIVE PCR,POLYMERASE CHAIN-REACTION,REAL-TIME PCR},
  language     = {eng},
  number       = {11},
  pages        = {1063--1067},
  title        = {The need for transparency and good practices in the qPCR literature},
  url          = {http://dx.doi.org/10.1038/nmeth.2697},
  volume       = {10},
  year         = {2013},
}

Chicago
Bustin, Stephen A, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, et al. 2013. “The Need for Transparency and Good Practices in the qPCR Literature.” Nature Methods 10 (11): 1063–1067.
APA
Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., et al. (2013). The need for transparency and good practices in the qPCR literature. NATURE METHODS, 10(11), 1063–1067.
Vancouver
1.
Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, et al. The need for transparency and good practices in the qPCR literature. NATURE METHODS. 2013;10(11):1063–7.
MLA
Bustin, Stephen A, Vladimir Benes, Jeremy Garson, et al. “The Need for Transparency and Good Practices in the qPCR Literature.” NATURE METHODS 10.11 (2013): 1063–1067. Print.