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Translocation and turnover of rhizodeposit carbon within soil microbial communities of an extensive grassland ecosystem

(2014) PLANT AND SOIL. 376(1-2). p.61-73
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Abstract
A substantial amount of photosynthesized plant-C is allocated belowground in grassland ecosystems where it influences the structure and function of the soil microbial community with potential implications for C cycling and storage. We applied stable isotope probing of microbial PLFAs and repeated soil sampling in a grassland over a period of 1 year to assess the role of microbial communities in the cycling of rhizodeposit-C. Pulse-labeling with (CO2)-C-13 was performed in a grassland site near Gent (Belgium). Soil samples were taken 24 h, 1 week, 1 month, 4 months, 9 months and 1 year following labeling and analyzed for C-13 in soil, roots and microbial PLFAs. C enrichment of PLFAs occurred rapidly (within 24 h) but temporally varied across microbial groups. PLFAs indicative for fungi and gram-negative bacteria showed a faster C-13 uptake compared to gram-positive bacteria and actinomycetes. However, the relative C-13 concentrations of the latter communities increased after 1 week, while those of fungi decreased and those of gram-negative bacteria remained constant. PLFA C-13 mean residence times were much shorter for fungi compared to bacteria and actinomycetes. Our results indicate temporally varying rhizodeposit-C uptake by different microbial groups, and faster turnover rates of mycorrhizal versus saprotrophic fungi and fungi versus bacteria. Fungi appeared to play a major role in the initial processing and possible rapid channeling of rhizodeposit-C into the soil microbial community. Actinomycetes and gram-positive bacteria appeared to have a delayed utilization of rhizodeposit-C or to prefer other C sources upon rhizodeposition.
Keywords
Rhizodeposition, C-13 Pulse-labeling, Soil microbial community structure, SIP-PLFA, Microbial carbon turnover, ACTIVE RHIZOSPHERE MICROORGANISMS, ARBUSCULAR MYCORRHIZAL FUNGI, ELEVATED ATMOSPHERIC CO2, LIPID FATTY-ACIDS, UPLAND GRASSLAND, ORGANIC-MATTER, DYNAMICS, BIOMASS, BACTERIAL, FLOW

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Citation

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Chicago
Balasooriya Lekamalage, Wajira Kumuduni, Karolien Denef, Dries Huygens, and Pascal Boeckx. 2014. “Translocation and Turnover of Rhizodeposit Carbon Within Soil Microbial Communities of an Extensive Grassland Ecosystem.” Plant and Soil 376 (1-2): 61–73.
APA
Balasooriya Lekamalage, W. K., Denef, K., Huygens, D., & Boeckx, P. (2014). Translocation and turnover of rhizodeposit carbon within soil microbial communities of an extensive grassland ecosystem. PLANT AND SOIL, 376(1-2), 61–73.
Vancouver
1.
Balasooriya Lekamalage WK, Denef K, Huygens D, Boeckx P. Translocation and turnover of rhizodeposit carbon within soil microbial communities of an extensive grassland ecosystem. PLANT AND SOIL. 2014;376(1-2):61–73.
MLA
Balasooriya Lekamalage, Wajira Kumuduni, Karolien Denef, Dries Huygens, et al. “Translocation and Turnover of Rhizodeposit Carbon Within Soil Microbial Communities of an Extensive Grassland Ecosystem.” PLANT AND SOIL 376.1-2 (2014): 61–73. Print.
@article{4179560,
  abstract     = {A substantial amount of photosynthesized plant-C is allocated belowground in grassland ecosystems where it influences the structure and function of the soil microbial community with potential implications for C cycling and storage. We applied stable isotope probing of microbial PLFAs and repeated soil sampling in a grassland over a period of 1 year to assess the role of microbial communities in the cycling of rhizodeposit-C. 
Pulse-labeling with (CO2)-C-13 was performed in a grassland site near Gent (Belgium). Soil samples were taken 24 h, 1 week, 1 month, 4 months, 9 months and 1 year following labeling and analyzed for C-13 in soil, roots and microbial PLFAs. 
C enrichment of PLFAs occurred rapidly (within 24 h) but temporally varied across microbial groups. PLFAs indicative for fungi and gram-negative bacteria showed a faster C-13 uptake compared to gram-positive bacteria and actinomycetes. However, the relative C-13 concentrations of the latter communities increased after 1 week, while those of fungi decreased and those of gram-negative bacteria remained constant. PLFA C-13 mean residence times were much shorter for fungi compared to bacteria and actinomycetes. 
Our results indicate temporally varying rhizodeposit-C uptake by different microbial groups, and faster turnover rates of mycorrhizal versus saprotrophic fungi and fungi versus bacteria. Fungi appeared to play a major role in the initial processing and possible rapid channeling of rhizodeposit-C into the soil microbial community. Actinomycetes and gram-positive bacteria appeared to have a delayed utilization of rhizodeposit-C or to prefer other C sources upon rhizodeposition.},
  author       = {Balasooriya Lekamalage, Wajira Kumuduni and Denef, Karolien and Huygens, Dries and Boeckx, Pascal},
  issn         = {0032-079X},
  journal      = {PLANT AND SOIL},
  keyword      = {Rhizodeposition,C-13 Pulse-labeling,Soil microbial community structure,SIP-PLFA,Microbial carbon turnover,ACTIVE RHIZOSPHERE MICROORGANISMS,ARBUSCULAR MYCORRHIZAL FUNGI,ELEVATED ATMOSPHERIC CO2,LIPID FATTY-ACIDS,UPLAND GRASSLAND,ORGANIC-MATTER,DYNAMICS,BIOMASS,BACTERIAL,FLOW},
  language     = {eng},
  number       = {1-2},
  pages        = {61--73},
  title        = {Translocation and turnover of rhizodeposit carbon within soil microbial communities of an extensive grassland ecosystem},
  url          = {http://dx.doi.org/10.1007/s11104-012-1343-z},
  volume       = {376},
  year         = {2014},
}

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