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Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

Ruben Vanholme (UGent) , Igor Cesarino (UGent) , Katarzyna Rataj, Yuguo Xiao, Lisa Sundin (UGent) , Geert Goeminne (UGent) , Hoon Kim, Joanna Cross (UGent) , Kris Morreel (UGent) , Pedro Araujo, et al.
(2013) SCIENCE. 341(6150). p.1103-1106
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.
Keywords
PHENYLPROPANOID METABOLISM, MONOLIGNOL BIOSYNTHESIS, SECONDARY CELL-WALL, ACID, GROWTH, PHENOTYPE, 3'-HYDROXYLASE, TRANSCRIPTION FACTORS, THALIANA, DEPOSITION

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Chicago
Vanholme, Ruben, Igor Cesarino, Katarzyna Rataj, Yuguo Xiao, Lisa Sundin, Geert Goeminne, Hoon Kim, et al. 2013. “Caffeoyl Shikimate Esterase (CSE) Is an Enzyme in the Lignin Biosynthetic Pathway in Arabidopsis.” Science 341 (6150): 1103–1106.
APA
Vanholme, R., Cesarino, I., Rataj, K., Xiao, Y., Sundin, L., Goeminne, G., Kim, H., et al. (2013). Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. SCIENCE, 341(6150), 1103–1106.
Vancouver
1.
Vanholme R, Cesarino I, Rataj K, Xiao Y, Sundin L, Goeminne G, et al. Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. SCIENCE. 2013;341(6150):1103–6.
MLA
Vanholme, Ruben, Igor Cesarino, Katarzyna Rataj, et al. “Caffeoyl Shikimate Esterase (CSE) Is an Enzyme in the Lignin Biosynthetic Pathway in Arabidopsis.” SCIENCE 341.6150 (2013): 1103–1106. Print.
@article{4179279,
  abstract     = {Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.},
  author       = {Vanholme, Ruben and Cesarino, Igor and Rataj, Katarzyna and Xiao, Yuguo and Sundin, Lisa and Goeminne, Geert and Kim, Hoon and Cross, Joanna and Morreel, Kris and Araujo, Pedro and Welsh, Lydia and Haustraete, Jurgen and McClellan, Christopher and Vanholme, Bartel and Ralph, John and Simpson, Gordon G and Halpin, Claire and Boerjan, Wout},
  issn         = {0036-8075},
  journal      = {SCIENCE},
  keyword      = {PHENYLPROPANOID METABOLISM,MONOLIGNOL BIOSYNTHESIS,SECONDARY CELL-WALL,ACID,GROWTH,PHENOTYPE,3'-HYDROXYLASE,TRANSCRIPTION FACTORS,THALIANA,DEPOSITION},
  language     = {eng},
  number       = {6150},
  pages        = {1103--1106},
  title        = {Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis},
  url          = {http://dx.doi.org/10.1126/science.1241602},
  volume       = {341},
  year         = {2013},
}

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