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Clathrin adaptor complex AP-2 mediates endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis

Simone Di Rubbo (UGent) , Niloufer Irani (UGent) , Soo Youn Kim, Zheng-Yi Xu, Astrid Gadeyne (UGent) , Wim Dejonghe (UGent) , Isabelle Vanhoutte (UGent) , Geert Persiau (UGent) , Dominique Eeckhout (UGent) , Sibu Simon (UGent) , et al.
(2013) PLANT CELL. 25(8). p.2986-2997
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Abstract
Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptor-mediated endocytosis.
Keywords
COATED VESICLES, PROTEIN LEEIX2, GENE EXPRESSION, SIGNAL TRANSDUCTION, DEPENDENT ENDOCYTOSIS, STRUCTURAL EXPLANATION, TANDEM AFFINITY PURIFICATION, PLANT CELLS, CONSTITUTIVE ENDOCYTOSIS, PLASMA MEMBRANE

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Chicago
Di Rubbo, Simone, Niloufer Irani, Soo Youn Kim, Zheng-Yi Xu, Astrid Gadeyne, Wim Dejonghe, Isabelle Vanhoutte, et al. 2013. “Clathrin Adaptor Complex AP-2 Mediates Endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis.” Plant Cell 25 (8): 2986–2997.
APA
Di Rubbo, S., Irani, N., Kim, S. Y., Xu, Z.-Y., Gadeyne, A., Dejonghe, W., Vanhoutte, I., et al. (2013). Clathrin adaptor complex AP-2 mediates endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis. PLANT CELL, 25(8), 2986–2997.
Vancouver
1.
Di Rubbo S, Irani N, Kim SY, Xu Z-Y, Gadeyne A, Dejonghe W, et al. Clathrin adaptor complex AP-2 mediates endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis. PLANT CELL. 2013;25(8):2986–97.
MLA
Di Rubbo, Simone, Niloufer Irani, Soo Youn Kim, et al. “Clathrin Adaptor Complex AP-2 Mediates Endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis.” PLANT CELL 25.8 (2013): 2986–2997. Print.
@article{4179094,
  abstract     = {Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptor-mediated endocytosis.},
  author       = {Di Rubbo, Simone and Irani, Niloufer and Kim, Soo Youn and Xu, Zheng-Yi and Gadeyne, Astrid and Dejonghe, Wim and Vanhoutte, Isabelle and Persiau, Geert and Eeckhout, Dominique and Simon, Sibu and Song, Kyungyoung and Kleine-Vehn, J{\"u}rgen and Friml, Jiri and De Jaeger, Geert and Van Damme, Dani{\"e}l and Hwang, Inhwan and Russinova, Eugenia},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {COATED VESICLES,PROTEIN LEEIX2,GENE EXPRESSION,SIGNAL TRANSDUCTION,DEPENDENT ENDOCYTOSIS,STRUCTURAL EXPLANATION,TANDEM AFFINITY PURIFICATION,PLANT CELLS,CONSTITUTIVE ENDOCYTOSIS,PLASMA MEMBRANE},
  language     = {eng},
  number       = {8},
  pages        = {2986--2997},
  title        = {Clathrin adaptor complex AP-2 mediates endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.113.114058},
  volume       = {25},
  year         = {2013},
}

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